Preparation of mesh-like collagen scaffolds for tissue engineering

Abstract

Collagen is an attractive biomaterial to construct scaffolds for tissue engineering and biomedical applications. Mesh-like collagen scaffolds were prepared using a synthetic poly(D,L-lactic-co-glycolic acid) (PLGA) mesh as a template. Collagen...

Collagen-decorated electrospun scaffolds of unsaturated copolyesters for bone tissue regeneration

“…The overall analysis of thermal properties after bioconjugation of the polymer scaffolds with collagen highlighted one of the most important advantages of covalently binding collagen to polymers over other functionalization techniques, which is the changes in the crystalline structure of the polymer. Despite the satisfactory results related to improvement in cell adhesion and proliferation by changes in the physical surface in other functionalization methods (i.e., physical adsorption 31,34 ), bioconjugation is also able to tune both mechanical and thermal properties of the pre-designed scaffolds, promoting a stable attachment of collagen molecules on the polymer surface. EDC/ NHS bioconjugation method, for instance, was employed by Perez-Nava and coworkers 30 to bioconjugate collagen to an electrospun poly(vinyl alcohol) scaffold structure, where the resultant scaffolds presented significant improvements in mechanical behavior (i.e., Young's modulus, elongation at break and ultimate tensile strength), which would not be achieved through a simple physical coating, for example.…”

“…Materials such as polymers derived from natural monomers, [29][30][31][32] natural ceramics such as hydroxyapatite and tricalcium phosphate, 32,33 or composite materials have been widely employed as main components of scaffolds to further collagen-based surface functionalization. These collagen combinations can be developed via polymeric blends, coatings involving physical adsorption in porous structures, 31,34 or collagen covalently binding through bioconjugation. 30 Bioconjugation appears to be an advantageous strategy to bind collagen to scaffolds due to the high stability of the formed covalent bonds and the more efficient collagen attachment when compared to other functionalization methods.…”

Recent Tissue Engineering Approaches to Mimicking the Extracellular Matrix Structure for Skin Regeneration

“…As seen in commercial products, composites of materials with different morphologies are often used to achieve both the physical barrier function against infection and foreign invasion that the epidermal layer of the skin provides and the progressive neoformation and reconstruction of the dermal layer [ 64 , 65 ]. In the epidermal layer, meshes, ESP sheets, and films tend to be the materials of choice [ 66 , 67 ]. Miguel et al used an ESP sheet composed of SF/PCL as the top layer to mimic the dense architecture and water resistance of the epidermal layer, spinning it to a thickness of 0.05 to 1.5 mm, comparable to that of the human epidermal layer.…”

Graphene Oxide–Protein-Based Scaffolds for Tissue Engineering: Recent Advances and Applications

“…Because collagen is the most abundant structural protein in vertebrates and plays important roles in controlling cell functions such as cell adhesion, proliferation, and differentiation, collagen-based biomaterials have been extensively used to prepare porous scaffolds for engineering tissue [44]. Furthermore, collagen type I-based biomaterials and fiber processing techniques have been employed to mimic the natural microstructure of various tissues; thus, porous collagen scaffolds are being used in the tissue engineering of cartilage, meniscus, bone, ligament, nerve, skin, and related structures [45,46]. Despite its high biocompatibility, collagen exhibits poor physical and chemical stability, which includes low mechanical strength, increased sensitivity to enzymatic degradation, and low thermal stability.…”