Preparation of Lutein Nanoparticles by Glycosylation of Saccharides and Casein for Protecting Retinal Pigment Epithelial Cells

Abstract

Age-related macular degeneration (AMD), a leading cause of visual impairment in the aging population, lacks effective treatment options due to a limited understanding of its pathogenesis. Lutein, with its strong antioxidant properties and ability to mitigate AMD by absorbing ultraviolet (UV) rays, faces challenges related to its stability and bioavailability in functional foods. In this study, we aimed to develop delivery systems using protein−saccharide conjugates to enhance lutein delivery and protect adult retinal pigment epithelial (ARPE-19) cells against sodium iodate (NaIO 3 )-induced damage. Various saccharides, including mannose, galactose, lactose, maltose, dextran, and maltodextrin, were conjugated to casein via the Maillard reaction for lutein delivery. The resulting lutein-loaded nanoparticles exhibited small size and spherical characteristics and demonstrated improved thermal stability and antioxidant capacity compared to free lutein. Notably, these nanoparticles were found to be nontoxic, as evidenced by reduced levels of cellular reactive oxygen species production (167.50

Protection effect of lutein-loaded Pickering emulsion prepared via ultrasound-assisted Maillard reaction conjugates on dry age-related macular degeneration

“…The parameters of the Maillard reaction were based on our previous method with minor modification. 26 Casein (4% w/v) and mannose (1% w/v) were solubilized entirely in deionized water, and the pH was adjusted to 9.0 using NaOH (1 mol L −1 ). Subsequently, the samples were then heated at 90 °C in a water bath for 3 h, and cooled to room temperature in an ice bath.…”

Focusing on New Discoveries in Food Technology and Creating a New Future of Nutrition and Health: An Introduction to the 4th International Symposium of Food Science, Nutrition and Health in Dalian, China

“…Dr. Ming Du reported the current advances in the potential role of marine bioactive proteins and peptides in thrombosis prevention of nutrition and cardiovascular disease . Dr. Yukun Song discussed the preparation of lutein nanoparticles by glycosylation of saccharides and casein to protect retinal pigment epithelial cells . Dr. Chunhua Wu introduced the construction of fish oil-based Pickering emulsions stabilized by nanochitin/gliadin/resveratrol complex particles .…”