Preparation of liposomes using a Mini-Lab 8.30 H high-pressure homogenizer

Bachmann, Dieter; Brandl, Martin; Gregoriadis, Gregory

doi:10.1016/0378-5173(93)90422-c

Cited by 33 publications

(10 citation statements)

References 9 publications

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“…[30][31][32] The highpressure homogenization is a fluid mechanical process that involves the subdivision of vesicles into smaller sizes and occurs in a special homogenizing valve. 33 Since industrial-scale production of liposomes has become a reality, the range of liposome-preparation methods has been extended by a number of techniques. Among these are the heating method, spray-drying, freeze-drying, supercritical reverse-phase evaporation, and several modified ethanol-injection techniques that are increasingly attractive, as extensively reviewed in Laouini et al 34 Finally, the microfluidic-based method is an emerging technology for liposome synthesis that allows strict control …”

Section: Methods For the Preparation Of Liposomesmentioning

confidence: 99%

Liposomes as nanomedical devices

Bozzuto¹,

Molinari²

2015

IJN

1,735

1,114

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Since their discovery in the 1960s, liposomes have been studied in depth, and they continue to constitute a field of intense research. Liposomes are valued for their biological and technological advantages, and are considered to be the most successful drug-carrier system known to date. Notable progress has been made, and several biomedical applications of liposomes are either in clinical trials, are about to be put on the market, or have already been approved for public use. In this review, we briefly analyze how the efficacy of liposomes depends on the nature of their components and their size, surface charge, and lipidic organization. Moreover, we discuss the influence of the physicochemical properties of liposomes on their interaction with cells, half-life, ability to enter tissues, and final fate in vivo. Finally, we describe some strategies developed to overcome limitations of the “first-generation” liposomes, and liposome-based drugs on the market and in clinical trials.

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Section: Methods For the Preparation Of Liposomesmentioning

confidence: 99%

Liposomes as nanomedical devices

Bozzuto¹,

Molinari²

2015

IJN

1,735

1,114

View full text Add to dashboard Cite

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“…According to these studies, as the number of passes through the homogenizer increases, a critical condition is reached beyond which further passes result in a broadening of the distribution [14]. Cholesterol‐containing formulations have been shown to increase in size after five to ten passes [13]. The data shown in Figures 4(a) and 4(b) did not show such trends, but it is noted that our experiments were limited to a maximum of five passes.…”

Section: Resultsmentioning

confidence: 99%

Preparation of small unilamellar vesicles (SUV) and biophysical characterization of their complexes with poly‐l‐lysine‐condensed plasmid DNA

Maguire¹,

Zhang²,

Shamlou³

2003

Biotech and App Biochem

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Liposomes have numerous applications in the (bio)pharmaceutical industries as agents in the synthesis of new biomaterials for use in areas including gene delivery. There is currently a need to establish efficient scaleable methods for the manufacture of liposomes, and in the present paper we describe the operation of a new high-velocity jet homogenizer for downsizing of multilamellar large vesicles to produce small unilamellar vesicles (SUV). Measurements of size distribution of SUVs are presented and compared with mathematical simulations based on the solution of a population balance equation combined with computational-fluid-dynamics analysis of flow in the homogenizer. Anionic SUVs are produced by the new method and incubated with poly-L-lysine (PLL)-condensed plasmid DNA (pDNA) to generate complexes under different physico-chemical conditions. The colloidal properties of the resulting complexes, including their size and charge, are measured using a Zetasizer and the encapsulation efficiency is obtained experimentally using a Pico Green assay. The results show that between 85 and 95% of the PLL-pDNA condensed plasmids were encapsulated by the liposomes, the smaller liposomes being more effective in encapsulating the complexes.

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“…); methods based on replacement of organic solvent(s) by aqueous media; methods based on detergent removal; methods based on size transformation and fusion and methods based on pH adjustment (Domb 2007). Among these preparation methods, a mechanical microfluidization technique provides many advantages in terms of scalability to batch production (Bachmann et al 1993;Talsma et al 1989), reproducibility of narrow size distribution (Masson 1989), handling higher lipid concentration over sonication (Talsma et al 1989) and the preparation steps do not involve chemical substances, which is suitable for food application.…”

Section: Introductionmentioning

confidence: 99%

Feasibility study of lecithin nanovesicles as spacers to improve the solubility of milk protein concentrate powder during storage

Bansal

Truong

2016

Dairy Sci. & Technol.

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Progressive loss of solubility of high-protein dairy powders is an unsolved issue in the dairy industry. Recent research has found that the solubility loss of powders is mainly contributed by the loss of dissociability of casein micelles from the parent powder particles. One of the ways of maintaining solubility of powders is to reduce the rate of development of association between casein micelles, by introducing spacers between the micelles. In this work, feasibility of using lecithin nanovesicles (produced by microfluidization) as spacers to improve solubility of protein-rich dairy powders was investigated. Nanostructured high-protein dairy powders were manufactured by adding 1, 5 and 10% w/w (of the total solids) nanovesicle (~82 nm) dispersions to MPC80 suspensions and spray drying at 160°C inlet and 75°C outlet air temperature. The powders obtained were stored at 25 or 4°C and 0.23 water activity and analysed for solubility loss during storage. The biggest effect of addition of lecithin nanovesicles on solubility was seen after 90 days of storage at 25°C. Addition of nanovesicles at a level as low as 1 and 5% w/w (of the total solids) could improve the solubility of MPC80 by 13 and 30%, respectively during storage at 25°C for 90 days. As expected, the solubility of all powders did not change much during 180 days of storage at 4°C and remained above 90%.

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Preparation of liposomes using a Mini-Lab 8.30 H high-pressure homogenizer

Cited by 33 publications

References 9 publications

Liposomes as nanomedical devices

Liposomes as nanomedical devices

Preparation of small unilamellar vesicles (SUV) and biophysical characterization of their complexes with poly‐l‐lysine‐condensed plasmid DNA

Feasibility study of lecithin nanovesicles as spacers to improve the solubility of milk protein concentrate powder during storage

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