Preparation of liposomes: A comparative study between the double solvent displacement and the conventional ethanol injection—From laboratory scale to large scale

Sala, M.; Miladi, Karim; Agusti, Géraldine; Elaı̈ssari, Abdelhamid; Fessi, Hatem

doi:10.1016/j.colsurfa.2017.02.084

Cited by 43 publications

(22 citation statements)

References 23 publications

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“…This method is simple to follow, and the liposomes are easily separated out by evaporating the excess ethanol and water [27]. One challenge with this method is that it is difficult to obtain a homogenous mixture of same-sized liposomes as compared to other methods [28].…”

Section: Solvent Dispersion Methodsmentioning

confidence: 99%

“…The double emulsion technique for synthesizing liposomes is similar to that of ethanol injection in that it utilizes solvents to produce the liposomes. However, in double emulsion, two different solvents are used to create a more homogenous solution of liposomes than ethanol injection can provide [28]. In this method of synthesizing liposomes, phospholipids are added to ethanol as in ethanol injection.…”

Section: Double Emulsionmentioning

confidence: 99%

“…In this method of synthesizing liposomes, phospholipids are added to ethanol as in ethanol injection. Once this solution is agitated, it is then added to a second solvent that contains glycerin [28]. Another method of double emulsion involves using water-in oil-in water methods for encapsulating liposomes [29].…”

Section: Double Emulsionmentioning

confidence: 99%

“…This method helps to encapsulate delicate liposomes that could otherwise be easily degraded [30]. This method is preferred to just ethanol injection because it can produce a more homogenous mixture of vesicles, and it potentially provides a method for preparing liposomes on an industrial scale because of its ability to transform high concentrations of phospholipids into high quantities of liposomes [28]. Double emulsion is also favorable because it encapsulates the liposomes, controlling the release of the drug as well as protecting the liposomes in harsh environments, such as the gastrointestinal tract [29,30].…”

Section: Double Emulsionmentioning

confidence: 99%

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Liposomes; from synthesis to targeting macrophages

Powers¹,

Nosoudi²

2019

biomedicalresearch

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Liposomes are a proposed tool to use for cell and tissue specific drug targeting. Because of their phospholipid membranes and carrying capacity within their spheres, liposomes are of particular interest for clinical applications. Stealth liposomes are especially important for these clinical applications because they are able to avoid the body's immune system. When it comes to synthesizing these liposomes, there are two major categories of synthesis techniques including active and passive loading. This paper focuses on passive loading techniques which involve mechanical dispersion methods, solvent dispersion methods, and detergent removal. Each of these categories of passive loading techniques has several methods for preparation, all of which aim at producing a homogeneous solution of liposomes with a high loading efficiency. Beyond preparation techniques, this paper also explores the use of liposomes in targeting macrophages to reduce inflammation in various diseases. From this, it has been found that liposomes are able to effectively target macrophages as well as deliver drugs to macrophages that cause the cells to reverse from a pro-inflammatory response to an anti-inflammatory response. These findings are critical as they may lead to further targeting of liposomes to specific cell types to treat other diseases in humans. Liposomes StructureLiposomes are small, artificial vesicles that are made up of phospholipids and cholesterol, much like natural plasma membranes [3]. According to Lieberman, these vesicles are self-forming and can form into a single sphere or several

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Section: Solvent Dispersion Methodsmentioning

confidence: 99%

Section: Double Emulsionmentioning

confidence: 99%

Section: Double Emulsionmentioning

confidence: 99%

Section: Double Emulsionmentioning

confidence: 99%

See 2 more Smart Citations

Liposomes; from synthesis to targeting macrophages

Powers¹,

Nosoudi²

2019

biomedicalresearch

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“…Moreover, their subcellular size facilitates absorption of the active pharmaceutical ingredient [26,27]. Several techniques are currently being used for nanoencapsulation and these include emulsion solvent evaporation [28][29][30][31], nanoprecipitation [13,23,32], emulsion solvent diffusion [33][34][35][36][37], ethanol injection [38,39] and ionic gelation [40][41][42][43]. Of these, nanoprecipitation appears to be the most straightforward and reproducible technique, as it enables highly efficient drug encapsulation in conjunction with adequate physical stability.…”

Section: Introductionmentioning

confidence: 99%

Relationship between the process variables and physicochemical features of liquid-core nanocapsules produced via nanoprecipitation

2019

Biointerface Res Appl Chem

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This study investigates the relationship between the physicochemical features of liquid-core nanocapsules created via nanoprecipitation and the adopted process variables. The process variables evaluated are the amount of coating polymer (poly--caprolactone), the amount of stabilizer polymer (poloxamer-188) and the stirring velocity. A statistical response surface methodology is employed to analyze the effect of the process variables on the physicochemical variables of size, polydispersity, zeta potential and efficiency in encapsulating a non-polar drug (carbamazepine). The results show that, although the process variables do not have a statistically significant effect on the response variables, the amount of coating polymer, the amount of stabilizer, and the stirring rate are physicochemically relevant. It is also found that the nanocapsules can contain more than 98% of the model drug, but the efficiency of the drug released may be affected by the process conditions.

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