1963
DOI: 10.1001/archderm.1963.01590240026005
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Preparation of Isolated Sheets of Human Stratum Corneum

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Cited by 730 publications
(325 citation statements)
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“…Subcutaneous fat was carefully trimmed and the skin was immersed in distilled water at 60 ± 1 C for 2 min (Kligman & Christophers, 1963), after which SCE (stratum corneum and epidermis) was removed from the dermis using a dull scalpel blade. Epidermal membranes were dried in a desiccator at $25% relative humidity.…”
Section: Skin Membrane Preparationmentioning
confidence: 99%
“…Subcutaneous fat was carefully trimmed and the skin was immersed in distilled water at 60 ± 1 C for 2 min (Kligman & Christophers, 1963), after which SCE (stratum corneum and epidermis) was removed from the dermis using a dull scalpel blade. Epidermal membranes were dried in a desiccator at $25% relative humidity.…”
Section: Skin Membrane Preparationmentioning
confidence: 99%
“…Para los objetivos de este informe solamente nos interesaba averiguar el perfil del estrato córneo. Por eso utilizamos una técnica con la enzima tripsina para disgregar el estrato córneo del resto de la piel (Kligman & Christophers, 1963). El espectro del estrato córneo muestra que hay una máxima principal (con las coordenadas de lex = 290 nm y lem = 335 nm) que representa el triptófano.…”
Section: Resultados Y Discusiónunclassified
“…The top layer of skin was then scraped with a razor, resulting in an intact epidermal sheet which was then used for transport studies. 11 Skin was placed in a¯ow-through diffusion system (Permegear, Rieglesville, PA) with a surface area of 0.785 cm 2 . The skin was sandwiched between a donor and receiver chamber, with the epidermal side being exposed to the donor solution (erythromycin) and the dermal side in contact with the receiver¯uid.…”
Section: Methodsmentioning
confidence: 99%