Preparation of Isolated Sheets of Human Stratum Corneum

Kligman, Albert M.; Christophers, Enno

doi:10.1001/archderm.1963.01590240026005

Cited by 732 publications

(327 citation statements)

References 6 publications

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“…Subcutaneous fat was carefully trimmed and the skin was immersed in distilled water at 60 ± 1 C for 2 min (Kligman & Christophers, 1963), after which SCE (stratum corneum and epidermis) was removed from the dermis using a dull scalpel blade. Epidermal membranes were dried in a desiccator at $25% relative humidity.…”

Section: Skin Membrane Preparationmentioning

confidence: 99%

Design of solid lipid nanoparticles for caffeine topical administration

et al. 2014

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Caffeine is an interesting compound showing anticancer and protective effects upon topical administration, although its penetration through the skin is compromised by its hydrophilicity. Materials and methods: SLN-CAF were formulated by using a modification of the quasi-emulsion solvent diffusion technique (QESD) and characterized by PCS and DSC analyses. In vitro percutaneous absorption studies were effected using excised human skin membranes (i.e. Stratum Corneum Epidermis or SCE).Results: SLN-CAF were in a nanometric range (182.6 ± 8.4 nm) and showed an interesting payload value (75% ± 1.1). DSC studies suggest the presence of a well-defined system and the successful drug incorporation. Furthermore, SLN-CAF generated a significantly faster permeation than a control formulation over 24 h of monitoring. Discussion and conclusions: SLN-CAF were characterized by valid dimensions and a good encapsulation efficiency, although the active to incorporate showed a hydrophilic character. This result confirms the suitability of the formulation strategy employed in the present work. Furthermore, the in vitro evidence outline the key role of lipid nanoparticles in enhancing caffeine permeation through the skin.

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Section: Skin Membrane Preparationmentioning

confidence: 99%

Design of solid lipid nanoparticles for caffeine topical administration

et al. 2014

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“…Para los objetivos de este informe solamente nos interesaba averiguar el perfil del estrato córneo. Por eso utilizamos una técnica con la enzima tripsina para disgregar el estrato córneo del resto de la piel (Kligman & Christophers, 1963). El espectro del estrato córneo muestra que hay una máxima principal (con las coordenadas de lex = 290 nm y lem = 335 nm) que representa el triptófano.…”

Section: Resultados Y Discusiónunclassified

Fluorescencia de los Tejidos Queratinosos

2012

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RESUMEN: Utilizando una técnica de fluorescencia, registramos mapas de los fluoróforos presentes en varios tipos de tejidos queratinosos. Descubrimos que la fluorescencia de todas las muestras analizadas tienen rasgos en común. Observamos el aminoácido triptófano en todos los tejidos. Asimismo, nos parece que la máxima principal es debido a las kinureninas que son productos metabólicos y degradativos del triptófano. Esta máxima constituye un conglomerado de varias moléculas como L-kinurenina, 3-hidroxikinurenina y N-formilkinurenina. La proporción de kinureninas que tiene el triptófano depende del tipo de tejido y del grado de pigmentación. También, la longitud de onda de excitación y emisión depende de estos parámetros.PALABRAS CLAVE: Tejidos queratinosos; Cabello; Piel; Fluorescencia; Uña; Pezuña; Caparazón; Cuerno. INTRODUCCIÓNLos tejidos queratinosos contienen muchos cromóforos que dan fluorescencia en las regiones de radiación visible y ultravioleta. Generalmente, las queratinas se clasifican como suaves o duras. Este aspecto se determina por la composición física y química del tejido. Ejemplos de queratinas duras son el pelo, la uña, la garra, la lana, la pezuña y la pluma. Por otro lado, tenemos queratinas suaves como el estrato córneo de la piel donde hay células diferenciadas que contienen filamentos de queratina y no de los organelos metabólicos de las células normales. Estas células diferenciadas se llaman corneocitos y están situadas en una matriz de lípidos que constituye todo el estrato córneo.

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“…The top layer of skin was then scraped with a razor, resulting in an intact epidermal sheet which was then used for transport studies. 11 Skin was placed in a¯ow-through diffusion system (Permegear, Rieglesville, PA) with a surface area of 0.785 cm 2 . The skin was sandwiched between a donor and receiver chamber, with the epidermal side being exposed to the donor solution (erythromycin) and the dermal side in contact with the receiver¯uid.…”

Section: Methodsmentioning

confidence: 99%

Transdermal delivery of erythromycin lactobionate—implications for the therapy of gastroparesis

Brand

Lof

Quigley

1997

Aliment Pharmacol Ther

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Background: The treatment of many diseases may be complicated by abnormalities in gastric emptying. Gastric motor dysfunction may lead to unpredictable food and medication delivery to the small intestine, their site of absorption. Prokinetic agents improve gastric motility, but orally administered drugs are unreliably absorbed, thereby limiting their effectiveness. A method of delivering prokinetic agents which bypasses the gastrointestinal tract could lead to more effective treatment. Methods: Skin samples from rat, hairless mouse and man were placed in an in vitro diffusion chamber. The epidermal side of the skin was exposed to erythromycin lactobionate and passage of the drug across the skin sample monitored and quantitated by high‐performance liquid chromatography with UV detection. Results: Erythromycin passed across all skin types tested. Steady‐state flux across hairless mouse skin was greater than for rat, full thickness human skin and human epidermis. In the first 3 h following introduction of erythromycin lactobionate, 1.85 mg/cm2 crossed human epidermis. Given that a dose of 50 mg may exert prokinetic effects in vivo in man, increasing the patch size to ≈28 cm2 should provide therapeutic levels of drug within 3 h. Conclusions: Erythromycin lactobionate, when administered transdermally, can be delivered at levels sufficient to treat gastroparesis. This technique warrants in vivo investigation.

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Preparation of Isolated Sheets of Human Stratum Corneum

Cited by 732 publications

References 6 publications

Design of solid lipid nanoparticles for caffeine topical administration

Design of solid lipid nanoparticles for caffeine topical administration

Fluorescencia de los Tejidos Queratinosos

Transdermal delivery of erythromycin lactobionate—implications for the therapy of gastroparesis

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