Preparation of immobilized proteins covalently coupled through silane coupling agents to inorganic supports

Weetall, Howard H.

doi:10.1007/bf02916421

Cited by 259 publications

(149 citation statements)

References 74 publications

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“…To date, wet and gas-phase silane monolayer formations are methods of choice for modifying glass and oxide surfaces (10)(11)(12). However, these monolayers are not always straightforward to prepare, because of a complex condensation process at the interface and result very often in unpredictable molecular densities of exposed -functionalities.…”

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confidence: 99%

Monolayers of derivatized poly( l -lysine)-grafted poly(ethylene glycol) on metal oxides as a class of biomolecular interfaces

Ruiz-Taylor¹,

Martin²,

Zaugg³

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

157

114

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We report on the design and characterization of a class of biomolecular interfaces based on derivatized poly(L-lysine)-grafted poly-(ethylene glycol) copolymers adsorbed on negatively charged surfaces. As a model system, we synthesized biotin-derivatized poly(L-lysine)-grafted poly(ethylene glycol) copolymers, PLL-g-[(PEGm) (1؊x) (PEG-biotin)x], where x varies from 0 to 1. Monolayers were produced on titanium dioxide substrates and characterized by x-ray photoelectron spectroscopy. The specific biorecognition properties of these biotinylated surfaces were investigated with the use of radiolabeled streptavidin alone and within complex protein mixtures. The PLL-g-PEG-biotin monolayers specifically capture streptavidin, even from a complex protein mixture, while still preventing nonspecific adsorption of other proteins. This streptavidin layer can subsequently capture biotinylated proteins. Finally, with the use of microfluidic networks and protein arraying, we demonstrate the potential of this class of biomolecular interfaces for applications based on protein patterning. C ontrolling immobilization of biomolecules on surfaces, while preventing nonspecific adsorption of unwanted species, has become an important goal for monitoring specific biointeractions and binding of biomolecules or cells. Indeed, in diagnostic assays, biomaterial devices, and surface-related bioanalytical applications, nonspecific protein binding can often be the obstacle to higher sensitivity, reproducibility, or implant integration. Therefore, in past decades, many immobilization strategies have been established. These include physisorption to solid organic or inorganic supports (noncovalent coupling occurs by electrostatic and van der Waals forces), noncovalent chemisorption, and covalent immobilization on organic thin films of different molecular organization.Physisorption of biomolecules directly on the surface of inorganic substrate materials such as glass or organic coatings such as polymeric materials and adhesion layers (polylysine and nitrocellulose) probably constitutes the least technically challenging immobilization procedure. For instance, spotted microarrays of nucleic acids and, more recently, proteins are mostly based on physical adsorption (1). However, these methods suffer from some key limitations, such as their lack of control over the quantity and orientation of adsorbed biomolecules, and, hence, from lower reproducibility, lower interaction efficiencies, and high error rates. Moreover, additional passivation or blocking steps of the remaining sites are often required to limit the extent of nonspecific binding and protein denaturation. These are serious limitations for protein microarray applications.Attempts to control the biomolecular density and orientation of biomolecules at the solid-liquid interface to obtain better reproducibility have been undertaken through various strategies of covalent and site-specific immobilization. These include mostly immobilization via organic thin films such as selfassembled alkanethiol a...

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mentioning

confidence: 99%

Monolayers of derivatized poly( l -lysine)-grafted poly(ethylene glycol) on metal oxides as a class of biomolecular interfaces

Ruiz-Taylor¹,

Martin²,

Zaugg³

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

157

114

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“…Both immunological and surface adsorption studies suggest that proteins interact readily with silane, [11] silicone-modified [10,12] and silicone elastomer surfaces. Protein adsorption may be greater at the aqueous/silicone rubber interface than at other polymeric surfaces, although the degree of adsorption is protein-dependent.…”

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confidence: 99%

Protein-Silicone Interactions

Bartzoka

McDermott

Brook³

1999

Adv. Mater.

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Silicone polymers (polydimethylsiloxanes, (Me 2 SiO) n , PDMSs) have been exploited in a variety of personal care [1] and medically related [2] applications. For instance, liquid silicones are used as oral defoamers in anti-acid formulations, [3] and as carriers for cosmetics and deodorants; [4] silicone elastomers are used in medical-grade tubing, transdermal drug delivery patches, and implanted prostheses (an application that has caused much controversy); [5] and silicone±polyether copolymers are used in hair-care applications.[4] The properties that make silicones particularly appropriate for these applications include hydrophobicity, low glass transition temperature T g (typically less than ±120 C [6]

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“….) is a general way to modify the surface that has been used for a number of years [75,76]. Compounds containing an alcohol or a phenol group (monoethanolamine, p-aminophenol) [77] or polyaldehyde [78] can also be used as coupling agents with inorganic supports having surface hydroxy groups.…”

Section: Covalent Graftingmentioning

confidence: 99%

Enzyme‐Based Bioinorganic Materials

Forano

Prévot

2007

Bio‐inorganic Hybrid Nanomaterials

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Trends in research materials currently focus on innovation in the field of intelligent or smart materials [1]. Smart materials are materials that display functionalities able to detect a change in the near environment, answer to a stimulus, modify a property, store, transfer or convert a signal, in a sense behave like living cells. The design of smart materials focuses on the concept of biomimetic materials [2][3][4] in order to reproduce specific and selective properties not deliverable by standard chemical or physical processes. Hence, there has been much effort concentrated on the development of enzyme-based biohybrid materials. Enzyme-based biohybrid materials are under much investigation for the operation of physical or chemical functions. Enzymes display active and selective properties such as molecular recognition, complexation or carrier functions, and a wide range of catalytic reactions (hydrolysis, condensation, redox reaction, isomerization, reaction transfer). Enzymes are thus very attractive as an alternative to traditional catalysts when chemical routes are difficult to apply. Enzymes are able to catalyze regio-and stereo-selective reactions with rate accelerations up to 10-17 fold [17]. They are interesting materials for realization of sensing devices, recognition systems and nanoreactors with many potential industrial developments in biocatalysis [4] for the food industry or effluent treatment [5,6], in biomedical domains for medical implants or enzyme delivery [7], in bioanalysis and affinity chromatography [8,9], and in biosensor technology [10][11][12].Engineering the development of biohybrid enzyme-based materials requires solution of the limitations of the use of enzymes due to their intrinsic fragility against the various environmental stresses and their narrow range of working conditions (e.g. short pH and temperature range). Embedding enzymes in protective matrices in order to solve these problems has been envisaged for many years [13,14]. Immobilization of enzymes in solid supports is seen as a strategy to preserve the active site, to improve the performance of the enzyme, that is, its activity, reaction rate and long-term stability, and to allow enzyme reuse. Physical and chemical properties of the solid supports such as particle size, surface area, pore diameter, mechanical j443 Bio-inorganic Hybrid Nanomaterials. Edited

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Preparation of immobilized proteins covalently coupled through silane coupling agents to inorganic supports

Cited by 259 publications

References 74 publications

Monolayers of derivatized poly( l -lysine)-grafted poly(ethylene glycol) on metal oxides as a class of biomolecular interfaces

Monolayers of derivatized poly( l -lysine)-grafted poly(ethylene glycol) on metal oxides as a class of biomolecular interfaces

Protein-Silicone Interactions

Enzyme‐Based Bioinorganic Materials

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