Preparation of immobilized lactase. Continued studies on the enzymatic hydrolysis of lactose

Weetall, Howard H.; Havewala, N. B.; Pitcher, Wayne H.; Detar, Clarence C.; Vann, W. P.; Yaverbaum, Sidney

doi:10.1002/bit.260160510

Cited by 45 publications

(4 citation statements)

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“…By taking 3-hourly samples of fermentation product when using 35% concentration of glucoamylase enzyme and 9% of yeast, a model of SSF reaction kinetics was predicted based on Michaelis-Menten equation [11][12]. From Fig.…”

Section: /[S]mentioning

confidence: 99%

Bioethanol from Sorghum Grain (Sorghum bicolor) with SSF Reaction Using Biocatalyst Co-Immobilization Method of Glucoamylase and Yeast

et al. 2015

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The development of bioethanol production is being widely discussed both the development of methods and materials used. One of potential raw material for bioethanol production is white sorghum (Sorghum bicolor). It thrives in tropical countries and has fairly high starch content about 65% -71% typically. Thus, it can be used as raw material of bioethanol production. During this process, a simultaneous saccharification and fermentation reaction (SSF) produces more ethanol than conventional processes. The purpose of this study is to determine proper method for producing bioethanol from this raw material by comparing SSF reaction of NaOH-treated white sorghum seeds to untreated sorghum seeds. Beads biocatalyst was prepared by coimmobilization method of glucoamylase by the yeast (Saccharomyces cereviceae) in Na-Alginate 6%. They were varied with variable concentrations of yeast 5%, 7%, 9% and 25%, 35% of glucoamylase enzyme. The biocatalyst is more stable, producing more bioethanol and reducing contamination. The results indicated that white sorghum seeds with 0.1 M NaOH treatment produced more bioethanol than untreated sorghum seeds using beads biocatalyst with concentration of yeast 9% and 35% of enzyme. Maximum production of ethanol was 11.48% with the value of K m and V m are -0.0014 g/mL and 0.00245 g/mL.hour respectively. By overnight soaking in 0.1 M NaOH solution, the purity of the starch in the white sorghum seeds increased.

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Section: /[S]mentioning

confidence: 99%

Bioethanol from Sorghum Grain (Sorghum bicolor) with SSF Reaction Using Biocatalyst Co-Immobilization Method of Glucoamylase and Yeast

et al. 2015

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“…The same techniques as those used for cell immobilization are available. Immobilization of penicillin acylase [313], l-amino acid acylase [309], lactase [314], and glucose isomerase [312] have been applied to commercial production. The entrapment of soluble enzymes in a membrane-type reactor with simultaneous coenzyme regeneration is an alternative to the use of cell-free enzymes.…”

Section: Conversion With Cell-free Enzymes or Purified Enzymesmentioning

confidence: 99%

Biotechnology

Schlegel

Doelle

Fiechter

et al. 2000

Ullmann's Encyclopedia of Industrial Chemistry

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“…However, this additional parameter did significantly improve the fit of the model to the second set of experimental data at a 95% confidence level. The models were evaluated as nested models using the following criterion:23 ss, -ss,+, ss,+,/cn -(i + 01 sF(l,n -(i + 1);c) (7) where i is the number of parameters in the simpler model, SS, is the residual sum of squares for the model with i parameters and F(l, n -(i + l); c) is an F ratio with l and n -(i + 1) degrees of freedom at a c confidence level.…”

Section: G + (Es) a (Esg)mentioning

confidence: 99%

Lactose hydrolysis by immobilized β‐galactosidase in capillary bed reactor

Peterson

Hill

Amundson

1989

Biotech & Bioengineering

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The hydrolysis of lactose by immobilized beta-galactosidase was studied in a continuous-flow capillary bed reactor operating at 30 degrees C. Solutions containing 50, 100, and 150 g lactose and 0.5 g sodium acetate/L were fed to the reactor. Lactose conversions ranging from 24% to greater than 99% were achieved at reactor space times ranging from 0.06 to 6.3 min. These conversion data were successfully modeled in terms of a plug flow reactor model and a form of Michaelis-Menten kinetics which included competitive inhibition by both the alpha and beta forms of galactose.

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Preparation of immobilized lactase. Continued studies on the enzymatic hydrolysis of lactose

Cited by 45 publications

References 1 publication

Bioethanol from Sorghum Grain (Sorghum bicolor) with SSF Reaction Using Biocatalyst Co-Immobilization Method of Glucoamylase and Yeast

Bioethanol from Sorghum Grain (Sorghum bicolor) with SSF Reaction Using Biocatalyst Co-Immobilization Method of Glucoamylase and Yeast

Biotechnology

Lactose hydrolysis by immobilized β‐galactosidase in capillary bed reactor

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