Preparation ofde NovoGlobular Proteins Based on Proline Dendrimers

Abstract

A synthetic method for the preparation of protein-like globular dendrimers derived from a combination of proline, glycine and imidazolidin ring as branching unit is described. The methodology allows the synthesis of novel peptide dendrimers up to fourth generation. Dendrimers were synthesized by a convergent solid-phase peptide synthesis approach. The conformational properties of branched polyproline peptides and proline dendrimers were studied by CD experiments. CD data suggest conformational plasticity of branched peptides for PPI and PPII, and a stable well-defined secondary structure of proline dendrimers for PPII.

The study of pH-dependent complexation between gelatin and gum arabic by morphology evolution and conformational transition

“…The PPI helix is a right-handed helix in which all peptide bonds are cis-oriented (u ¼ 0 ) with 4 and j angles of À75 and þ160 , respectively. These dihedral angles form helical pitch of 5.6 Å per turn, resulting in a more compact structure with the amide bonds oriented nearly parallel to the PPI helical axis (Sanclimens, Crespo, Giralt, Albericio, & Royo, 2005). Kuemin elaborated the difference between PPII helix and PPI helix with schematic molecular models (Kuemin, Schweizer, Ochsenfeld, & Wennemers, 2009).…”

“…At pH 4.7, GA was in the form of the PPII helix; whereas, a PPI helix was found at pH 3.1, suggesting the alteration at the molecular level. Such switch of helix type generally happens upon the change of solvents: the organic solvent facilitates the conformation of PPI helix; whereas, in aqueous solution, they tend to adopt the PPII helix (Sanclimens et al, 2005). Although the intrinsic mechanism for the transition of GA conformation in GeGA system during complexation is difficult to disclose, the electrostatic interaction would probably be the driving force.…”

Branched Peptides for the Modulation of Protein-Protein Interactions: More Arms are Better than One?

“…In fact while dimeric or tetrameric peptides of moderate length (about 10-15 residues) are readily accessible by the solid phase method, the synthesis of higher order multimers, such as octamers, of the same length, generally requires ligation approaches in solution or on the solid phase [30][31][32].…”

Amino Acid‐Based Dendrimers

“…A number of other peptide dendrimers composed of glutamic/aspartic acid [19][20][21][22][23], proline [8,[24][25][26], or arginine [27][28][29] have been reported. Ranganathan et al synthesized several series of polyglutamic/aspartic acid dendrimers including those with adamantane, an oxalyl group, or a benzenetricarbonyl unit as dendrimer core (Scheme 15.3) [19][20][21].…”