Preparation of Hydrophilic Polyhydroxyalkyl Glutamine Crosslinked Films and its Biodegradability

Abstract

Polybenzyl glutamate (PBLG) or polymethyl glutamate (PMLG) films have been aminolyzed with amino alcohol and crosslinked with aliphatic diamine at 60 degrees C for 48 h simultaneously which led to the formation of crosslinked films of polyhydroxyalkyl glutamine (PHAG). ATR-IR indicates that for the aminolysis of PBLG with 2-amino-1-ethanol or 3-amino-1-propanol, benzyl glutamate almost completely turned to hydroxyalkyl glutamine, however for the aminolysis of PMLG with 5-amino-1-pentanol, methyl glutamate partially turned to hydroxypentanyl glutamine. The water-swelling test shows that water-swelling ratio Q of PHAG films from amino alcohol with longer carbon chain was smaller, the PHAG films crosslinked by 1,2-diamino ethane have the higher water-swelling ratio Q, but the PHAG films crosslinked by 1,8-diamino octane have the lower water swelling ratio Q; and PHAG films with a greater amount of crosslinking agents have high crosslinking density or the low water swelling-ratio Q for same amino alcohol and diamine. It is obvious from in vitro enzymatic hydrolysis test that specimens with smaller swelling ratio Q displayed larger T(1/2), time for half weight digestion of PHAG film, that is, less biodegradability. Therefore, biodegradability of the crosslinked PHAG films can be controlled by changing amino alcohol and diamine.

Efficient Intracellular Gene Delivery Using the Formulation Composed of Poly (L-glutamic Acid) Grafted Polyethylenimine and Histone

“…Polyethylenimine with average molecular weight of 800 Da (PEI 800) and 25 kDa (PEI 25 K) was purchased from Sigma-Aldrich. Poly-γ-benzyl-L-glutamate (PBLG) was synthesized in our laboratory, and the detailed synthesis can be found in Refs (20) and (21). The pEGFP-C1 plasmid was a gift from West China University of Medical Sciences.…”

Cellular uptake and transfection activity of DNA complexes based on poly(ethylene glycol)-poly(l-glutamine) copolymer with PAMAM G2

“…BLG-NCA was prepared according to the literature. 18 Briey, BLG (10.0 g, 0.042 mol) and triphosgene (6.0 g, 0.020 mol) were added into 100 mL of dried tetrahydrofuran. Aer stirring at 65 C for 30 min, the suspension turned transparent.…”

Salt-Induced Stability and Serum-Resistance of Polyglutamate Polyelectrolyte Brushes/Nuclear Factor-κB p65 siRNA Polyplex Enhance the Apoptosis and Efficacy of Doxorubicin

“…Glutamic acid and N , N -dimethylformamide (DMF) were obtained from Sinopharm Chemical Reagent Co.. Spermine was purchased from Aladdin Chemistry Co. Ltd. Poly-γ-benzyl- l -glutamate (PBLG) was synthesized by using the molar ratio of monomer to initiator of 100:1 in our laboratory and the detailed synthesis can be found in refs and . The HeLa cell line was obtained from the American Type Culture Collection (ATCC).…”