Preparation of hydrogel embedded polymer-growth factor conjugated nanoparticles as a diabetic wound dressing

Abstract

A polymer conjugated rhEGF was developed that was more stable against proteases and reserved the biological activity of the drug. This dressing appears to be a competent candidate for chronic wound healing.

Advanced hydrogels for treatment of diabetes

“…In addition, the re-epithelization and granulation of infected wounds in diabetic mice were accelerated after treatment with the hydrogel.Hajimiri et al, designed a human recombinant EGF (rhEGF)-conjugated sodium carboxymethyl chitosan hydrogel to extend the lifespan of rhEGF. This formulation accelerated the closure of wounds in a murine diabetic model within 2 weeks after treatment, yielding results comparable with those under treatment with the commercially available wound dressing Comfeel®, which lacks of rhEGF but is capable of absorbing exudate from wounds without adhere(Hajimiri et al, 2016).Similarly, a heparin-mimetic amphiphilic peptide (PA) hydrogel effectively accelerated the healing of wounds in vivo. The oligopeptides PALauryl-VVAGEGDK(pbs)S-Am (GAG-PA) and Lauryl-VVAGK-Am (K-PA), interact with each other covalently to form a fibrillar hydrogel having a net negative charge capable to bind VEGF.…”

“…Hajimiri et al, designed a human recombinant EGF (rhEGF)‐conjugated sodium carboxymethyl chitosan hydrogel to extend the lifespan of rhEGF. This formulation accelerated the closure of wounds in a murine diabetic model within 2 weeks after treatment, yielding results comparable with those under treatment with the commercially available wound dressing Comfeel®, which lacks of rhEGF but is capable of absorbing exudate from wounds without adhere (Hajimiri et al, ).…”

Innovations in gene and growth factor delivery systems for diabetic wound healing

“…The nanoparticles employed in the studies were formed by either chemically conjugating the GFs with polymers [e.g. rhEGF-sodium carboxymethyl chitosan (Hajimiri et al, 2016) or by recombinantly fusing them [e.g. KGF (Koria et al, 2011) or stromalderived factor-1 (SDF-1) (Yeboah et al, 2016)] with elastin-like peptides (ELP).…”

“…KGF (Koria et al, 2011) or stromalderived factor-1 (SDF-1) (Yeboah et al, 2016)] with elastin-like peptides (ELP). Clearly, the emphasis of these studies was with the development of these therapeutic nanoparticles, that offered improved resistance to degradation by protease [savinase (Hajimiri et al, 2016) and elastase (Yeboah et al, 2016)] thereby enhancing the therapeutic effect in vivo.…”

Nanoparticles and nanosuspensions: physical and biological properties

“…Original research using curcumin loaded nanoparticles for in vivo assessment of treatment of colon cancer is reported 2 . Other diverse applications of nanoparticles are presented here including improving treatment of diabetic wound therapy 3 , and antibacterial activity 4 . In terms of biological characterization, solid lipid nanoparticles are evaluated for cellular and mitochondrial uptake 5 .…”