Preparation of human saphenous vein for coronary artery bypass grafting impairs its capacity to produce prostacyclin

Abstract

Prostacyclin production was measured from freshly isolated human saphenous vein and from vein subjected to routine surgical preparation for coronary bypass grafting. Surgical preparation had no effect on spontaneous prostacyclin production but significantly reduced stimulated rates from 16.9(1.1) to 7.1(0.5) pg.min-1 per mg wet weight (n = 27). Stimulated prostacyclin production was not reduced by storage of vein for 2 h at 23 degrees C in blood or saline nor by distension, but it was reduced to 5.0(0.6) pg.min-1 per mg (n = 10) by de-endothelialisation. Reduced prostacyclin production, which might in itself contribute to vein graft occlusion, provides a quantitative biochemical estimate of endothelial integrity.

Influence of endothelium and surgical preparation on responses of human saphenous vein and internal thoracic artery to angiotensin II.

“…The lesser response of the endothelialized FSV could be due to release of a relaxing substance from '01~~~~~~~~~t he endothelium and A II is known to release PG12 from vascular endothelium of some isolated tissues [17]. It has also been reported that mechanically stimulated PG12 release from SV is impaired by surgical preparation [17,18]. For these reasons the release of this vasodilator from the rings of SV was examined.…”

Manual pressure distension of the human saphenous vein changes its biomechanical properties—implication for coronary artery bypass grafting

“…Previous studies have demonstrated that uncontrolled manual distension of the saphenous vein structurally and functionally damage the endothelium (Angelini et al, 1987a, b;Chello et al, 2003;Johnson et al, 2001;Mills and Everson, 1995;Svendsen et al, 1986;Thatte and Khuri, 2001), muscle (Angelini et al, 1985(Angelini et al, , 1987aCornelissen et al, 2004;George et al, 1997;O'Brien et al, 1998), and interstitium (Kennedy et al, 1989a, b). In the present study, the wall thickness and wall area were largest in the saphenous vein with uncontrolled manual distension.…”

Reciprocal inhibition of nitric oxide and prostacyclin synthesis in human saphenous vein

“…An important practical corollary of this interplay between endothelium and smooth muscle is the belief that the endothelial function of the human saphenous vein, the conduit used most commonly for coronary artery bypass surgery (CABS), is a determining factor in the long term patency of the blood vessel after grafting. This is attributed largely to the ability of NO and PGI2 to modulate smooth muscle tone and to prevent thrombosis and smooth muscle proliferation (Angelini et al, 1987;1989;Painter, 1990). In the saphenous vein, release of these endothelium-derived agonists can be stimulated by, and therefore can modify the actions of, a variety of endogenous vasoactive substances including leukotriene C4 (LTC4), leukotriene D4 (LTD4) and histamine (Yang et al, 1989;Allen et al, 1992).…”