In this paper we demonstrate physical method (Gamma irradiation technique) for synthesizing Ag-NPs when there is no reduction agent or heat treatment as useful in antimicrobial applications and medical devices. As a strong support, stabilizer, and silver precursor, Chitosan (CS) and AgNO3 were utilized. Ag/Cs characteristics were investigated as a function of gamma dose and -irradiation periods in recent published paper. For conducting the research in Ag-CS-Isopropanol (IPA) by gamma Irradiation method, here we used UV-vis spectroscopy for examining energy gap and Plasmon resonance of surface (SPR) of the produced silver nanoparticles (Ag-NPs). Manufactured Ag-NP UV-vis spectroscopy revealed as the gamma dose increased, the highest wavelength intensity of the plasmon peaks rose. and with energy gap in rang (2.1-2.4) eV. The effect of Ag/CS-APA solutions has examined due on human blood properties and antibacterial activity to water samples selected from local riverbank in Iraq. Overall, the optical characteristic of Ag-CS-APA nanocomposite solution make them promising material for optical thin films and as antibacterial agent in health sector.