Preparation of giant vesicles by external AC electric fields. Kinetics and applications

Angelova, Maria; Soléau, S.; Méléard, Philippe; Faucon, F.; Bothorel, P.

doi:10.1007/bfb0116295

Cited by 737 publications

(614 citation statements)

References 12 publications

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“…3 Differences in membrane folding between the sample with pure POPC and the mixture of POPC and cardiolipin could be observed throughout the process. Pure POPC membranes were mostly arranged into spherelike segments (Figure 2A).…”

Section: Resultsmentioning

confidence: 99%

Shape and Size of Giant Unilamellar Phospholipid Vesicles Containing Cardiolipin

Tomšiè

Babnik

Lombardo

et al. 2005

J. Chem. Inf. Model.

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The effect of cardiolipin content on the shape and size of giant palmitoyloleylphosphatidylcholine/cardiolipin vesicles was studied. Unilamellar vesicles were prepared in sugar solution by the method of electroformation, from mixtures containing up to 50% weight ratio of cardiolipin. At room temperature the vesicles containing cardiolipin exhibited abrupt changes in the curvature of the vesicle contour indicating regions of phase separation. The deviations from the spherical shape were larger if vesicles were made from mixtures with a higher content of cardiolipin. Numerous vesicles with soft fluctuating walls were observed. The estimated size of the vesicles containing cardiolipin was found to be smaller than the size of pure palmitoyloleylphosphatidylcholine vesicles.

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Section: Resultsmentioning

confidence: 99%

Shape and Size of Giant Unilamellar Phospholipid Vesicles Containing Cardiolipin

Tomšiè

Babnik

Lombardo

et al. 2005

J. Chem. Inf. Model.

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“…Twenty microliters of lipids in 1:10 chloroform/methanol (2 mg/ml DOPC, 0.067 mg/ml DOPE-Rh, and 0.043 mg/ml DOPE-Bio) were dropped onto one of two indium tin oxidecoated glass slides (4 cm × 6 cm). The lipids were distributed on the glass by the "rock and roll" method (22) and dried for 1 hr under continuous nitrogen flow. A 1-ml volume chamber was constructed from the two glass plates, the dried lipids on the bottom glass, and a polydimethylsiloxane (PDMS) spacer.…”

Section: Methodsmentioning

confidence: 99%

“…A 1-ml volume chamber was constructed from the two glass plates, the dried lipids on the bottom glass, and a polydimethylsiloxane (PDMS) spacer. The chamber was filled with a solution of 200 mM sucrose and an AC voltage applied to the glass plates, forming GUVs by the electroformation method (22).…”

Section: Methodsmentioning

confidence: 99%

Bidirectional membrane tube dynamics driven by nonprocessive motors

Shaklee

Idema

Koster

et al. 2008

Proc. Natl. Acad. Sci. U.S.A.

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In cells, membrane tubes are extracted by molecular motors. Although individual motors cannot provide enough force to pull a tube, clusters of such motors can. Here, we investigate, using a minimal in vitro model system, how the tube pulling process depends on fundamental properties of the motor species involved. Previously, it has been shown that processive motors can pull tubes by dynamic association at the tube tip. We demonstrate that, remarkably, nonprocessive motors can also cooperatively extract tubes. Moreover, the tubes pulled by nonprocessive motors exhibit rich dynamics as compared to those pulled by their processive counterparts. We report distinct phases of persistent growth, retraction, and an intermediate regime characterized by highly dynamic switching between the two. We interpret the different phases in the context of a single-species model. The model assumes only a simple motor clustering mechanism along the length of the entire tube and the presence of a length-dependent tube tension. The resulting dynamic distribution of motor clusters acts as both a velocity and distance regulator for the tube. We show the switching phase to be an attractor of the dynamics of this model, suggesting that the switching observed experimentally is a robust characteristic of nonprocessive motors. A similar system could regulate in vivo biological membrane networks.collective behavior | bidirectionality | Ncd | cooperativity

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“…To grow the GUVs, a special temperature-controlled chamber was used, which was described previously. 44 The experiments were carried out in the same chamber after vesicle formation, by use of an inverted microscope (Axiovert 35; Zeiss, Thornwood, NY). The following steps were used to prepare the GUVs: (1) The lipid solution (2 µL) was spread on each platinum (Pt) wire under a stream of N2.…”

Section: Methodsmentioning

confidence: 99%

Visualizing Association of N-Ras in Lipid Microdomains: Influence of Domain Structure and Interfacial Adsorption

et al. 2005

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Abstract:In this study, two-photon fluorescence microscopy on giant unilamellar vesicles and tappingmode atomic force microscopy (AFM) are applied to follow the insertion of a fluorescently (4,4-difluoro-4-bora-3a,4a-diaza-s-indacene, BODIPY) labeled and completely lipidated (hexadecylated and farnesylated) N-Ras protein into heterogeneous lipid bilayer systems. The bilayers consist of the canonical raft mixture 1-palmitoyl-2-oleoylphosphatidylcholine (POPC), sphingomyelin, and cholesterol, whichsdepending on the concentration of the constituentssseparates into liquid-disordered (ld), liquid-ordered (lo), and solid-ordered (so) phases. The results provide direct evidence that partitioning of N-Ras occurs preferentially into liquiddisordered lipid domains, which is also reflected in a faster kinetics of incorporation into the fluid lipid bilayers. The phase sequence of preferential binding of N-Ras to mixed-domain lipid vesicles is ld > lo . so. Intriguingly, we detect, using the better spatial resolution of AFM, also a large proportion of the lipidated protein located at the ld/lo phase boundary, thus leading to a favorable decrease in line tension that is associated with the rim of the demixed phases. Such an interfacial adsorption effect may serve as an alternative vehicle for association processes of signaling proteins in membranes.

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Preparation of giant vesicles by external AC electric fields. Kinetics and applications

Cited by 737 publications

References 12 publications

Shape and Size of Giant Unilamellar Phospholipid Vesicles Containing Cardiolipin

Shape and Size of Giant Unilamellar Phospholipid Vesicles Containing Cardiolipin

Bidirectional membrane tube dynamics driven by nonprocessive motors

Visualizing Association of N-Ras in Lipid Microdomains: Influence of Domain Structure and Interfacial Adsorption

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