Preparation of gellan sulfate as an artificial ligand for removal of extra domain A containing fibronectin

Miyamoto, Keiichi; Asakawa, Youichi; Arai, Youko; Shimizu, Takeo; Tokita, Masayuki; Komai, Takashi

doi:10.1016/s0141-8130(01)00135-0

Cited by 20 publications

(6 citation statements)

References 3 publications

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“…The use of gellan gum in cartilage tissue engineering was pioneered by Oliveira et al and this gum has been successfully modified into an injectable cell‐laden hydrogel by oxidative cleavage as reported previously . Gellan sulfate (GS) was originally prepared by our group . Because GS has a heparin‐like activity, it has the capacity to bind heparin‐binding proteins such as TNC.…”

Section: Discussionmentioning

confidence: 99%

Effect of tenascin‐C on the repair of full‐thickness osteochondral defects of articular cartilage in rabbits

Ikemura

Hasegawa

Iino

et al. 2015

Journal Orthopaedic Research

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The purpose of this study was to examine the effect of tenascin-C (TNC) on the repair of full-thickness osteochondral defects of articular cartilage in vivo. We used a gellan-gellan-sulfate sponge (Gellan-GS) to maintain a TNC-rich environment in the cartilage defects. We implanted Gellan-GS soaked in PBS only (Group 1), Gellan-GS soaked in 10 mg/ml of TNC (Group 2), and Gellan-GS soaked in 100 mg/ml of TNC (Group 3) into a full-thickness osteochondral defect of the patellar groove of rabbits. The defect area was examined grossly and histologically 4-12 weeks after surgery. Sections of synovium were also immunohistochemically investigated. Histologically as well as macroscopically, the defects in Group 2 showed better repair than the other groups at 8 and 12 weeks after surgery. Inflammation of the synovium tended to diminish over time in all groups, and the degree of synovitis was the same for all three groups at each time point. In conclusion, Gellan-GS soaked in TNC can be used as a novel scaffold for the repair of articular cartilage defects. This study also indicates that TNC promotes the repair of full-thickness osteochondral defects in vivo. ß

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Section: Discussionmentioning

confidence: 99%

Effect of tenascin‐C on the repair of full‐thickness osteochondral defects of articular cartilage in rabbits

Ikemura

Hasegawa

Iino

et al. 2015

Journal Orthopaedic Research

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“…Gellan sulfate has been prepared for use in various medical applications, for example, as an artificial ligand to remove the extra domain A containing fibronectin (EDA(+)FN) from the plasma of rheumatoid arthritis patients42, and as a novel anticoagulant43.…”

Section: Discussionmentioning

confidence: 99%

“…However, unlike heparin, gellan sulfate is a potential artificial ligand for EDA(+)FN from the plasma of rheumatoid arthritis patients because it does not bind to proteins such as plasma fibronectin and antithrombin III42. Yet, gellan sulfates with 17.5% sulfation or greater were found to be potent anticoagulants, like heparin in APTT assays by Miyamoto et al 43.…”

Section: Discussionmentioning

confidence: 99%

“…Gellan gum has been modified to obtain sulfated derivatives (gellan sulfate)42 that have anticoagulant activities similar to those of heparin, with the exception of one derivative (GS1), which had the lowest sulfonation ratio (5.0%), and the least anticoagulant activity43. The in vivo effects of gellan sulfate, however, appear not to have been fully explored yet.…”

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confidence: 99%

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Gellan sulfate inhibits Plasmodium falciparum growth and invasion of red blood cells in vitro

Recuenco

Kobayashi

Ishiwa

et al. 2014

Sci Rep

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Here, we assessed the sulfated derivative of the microbial polysaccharide gellan gum and derivatives of λ and κ-carrageenans for their ability to inhibit Plasmodium falciparum 3D7 and Dd2 growth and invasion of red blood cells in vitro. Growth inhibition was assessed by means of flow cytometry after a 96-h exposure to the inhibitors and invasion inhibition was assessed by counting ring parasites after a 20-h exposure to them. Gellan sulfate strongly inhibited invasion and modestly inhibited growth for both P. falciparum 3D7 and Dd2; both inhibitory effects exceeded those achieved with native gellan gum. The hydrolyzed λ-carrageenan and oversulfated κ-carrageenan were less inhibitory than their native forms. In vitro cytotoxicity and anticoagulation assays performed to determine the suitability of the modified polysaccharides for in vivo studies showed that our synthesized gellan sulfate had low cytotoxicity and anticoagulant activity.

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“…In medicine field, the use of gellan has been investigated for tissue engineering (e.g., for cartilage reconstruction and guided bone regeneration) and wound healing (e.g., in wound dressings to inhibit postsurgical adhesion and scar formation). Gellan sulfate derivatives are promising materials for rheumatoid arthritis treatment, as they have a tendency for selective binding of fibronectin molecules ( Miyamoto et al, 2001 ), and for the development of cell-hybrid materials for artificial veins design due to the anticoagulant activity of such derivatives ( Miyamoto et al, 2002 ).…”

Section: Industrial and Biomedical Applicationsmentioning

confidence: 99%

Exopolysaccharides enriched in rare sugars: bacterial sources, production, and applications

et al. 2015

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Microbial extracellular polysaccharides (EPS), produced by a wide range of bacteria, are high molecular weight biopolymers, presenting an extreme diversity in terms of chemical structure and composition. They may be used in many applications, depending on their chemical and physical properties. A rather unexplored aspect is the presence of rare sugars in the composition of some EPS. Rare sugars, such as rhamnose or fucose, may provide EPS with additional biological properties compared to those composed of more common sugar monomers. This review gives a brief overview of these specific EPS and their producing bacteria. Cultivation conditions are summarized, demonstrating their impact on the EPS composition, together with downstream processing. Finally, their use in different areas, including cosmetics, food products, pharmaceuticals, and biomedical applications, are discussed.

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Preparation of gellan sulfate as an artificial ligand for removal of extra domain A containing fibronectin

Cited by 20 publications

References 3 publications

Effect of tenascin‐C on the repair of full‐thickness osteochondral defects of articular cartilage in rabbits

Effect of tenascin‐C on the repair of full‐thickness osteochondral defects of articular cartilage in rabbits

Gellan sulfate inhibits Plasmodium falciparum growth and invasion of red blood cells in vitro

Exopolysaccharides enriched in rare sugars: bacterial sources, production, and applications

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