Preparation of gelatin scaffold and fibroblast cell culture

Abstract

A gelatin gel sheet was UV-irradiated to raise the gel melting temperature T m . Three second of T m increased proportionally to irradiation time t UV . The sheet of which T m was larger than the culture temperature 37°C was used as a cell culture scaffold. Human fibroblast cells WI-38 adhered to the sheet and dug deeply into it regardless of t UV , while cancer cells WI-38-VA-13 formed spheroids and did not adhere to the sheet individually at high UV dose. The cell selectivity of the scaffold could be used for biological and medical studies.

Frank–Kasperσphase in polybutadiene-poly(ε-caprolactone) diblock copolymer/polybutadiene blends

“…One can form blend samples simply by mixing a block copolymer with a homopolymer. According to current research, the σ phase in blends exists widely in phase diagrams [39]. Thus, the σ phase can be observed easily by means of a simple blend method.…”