Abstract:Gelatin nanoparticles have recently been receiving considerable attention because they offer a good option as release systems due to their low cost, biocompatibility, biodegradability and its application in several types of formulations. This study aim was to evaluate the potential application of gelatin nanoparticles entrapping a photosensitizer in Photodynamic Therapy. Gelatin nanoparticles were studied by steady-state techniques and the biological activity evaluated by in vitro MTT assay. The particles were… Show more
“…In this respect, encapsulation of both component in a nanostructure would allow better energy transfer between the two partners and more incisive cytotoxic effect. Additionally, encapsulation in nanostructures allows gaining access to the well‐known features of passive and active targeting, thus leading in perspective to more efficient and selective delivery in target tissues …”
Section: Methodsmentioning
confidence: 99%
“…Additionally, encapsulation in nanostructures allows gaining access to the well-known features of passive and active targeting, thus leading in perspective to more efficient and selective delivery in target tissues. [17][18][19][20][21] In this study, we co-encapsulated a photosensitizer, chlorophyllin-copper complex, and CdSe/ZnS core-shell QDs as energy donors into biodegradable polymeric NPs composed of poly (lactide-co-glycolide) (PLGA). We investigated the morphological and photophysical properties of NPs, with special attention to the QD fluorescence properties changes upon encapsulation, then we studied the ability of this formulation to generate ROS in an aqueous environment and in living cells.…”
Section: Poly(lactide-co-glycolide) Nanoparticles Co-loaded With Chlomentioning
Photodynamic therapy (PDT) is an approach to treating cancer and involves light‐induced activation of a photosensitizer that triggers the formation of reactive oxygen species (ROS) in targeted cells and subsequent cell death. Examples of photosensitizers are porphyrins, including the natural compound chlorophyll. These molecules can be delivered alone or co‐formulated with an agent, such as quantum dots (QDs), that is able to excite them through a fluorescence resonance energy transfer (FRET)‐based mechanism. We encapsulated a chlorophyllin copper complex and CdSe/ZnS core‐shell QDs into biodegradable nanoparticles (NPs) composed of poly(lactide‐co‐glycolide) (PLGA), that allow modification with specific targeting ligands. When excited at 365 nm, FRET occurs between co‐encapsulated QDs and chlorophyllin to result in the formation of ROS. This chlorophyllin‐QD coformulation allows generation of ROS both in an aqueous environment and in cells, thus confirming the potential of this formulation in PDT.
“…In this respect, encapsulation of both component in a nanostructure would allow better energy transfer between the two partners and more incisive cytotoxic effect. Additionally, encapsulation in nanostructures allows gaining access to the well‐known features of passive and active targeting, thus leading in perspective to more efficient and selective delivery in target tissues …”
Section: Methodsmentioning
confidence: 99%
“…Additionally, encapsulation in nanostructures allows gaining access to the well-known features of passive and active targeting, thus leading in perspective to more efficient and selective delivery in target tissues. [17][18][19][20][21] In this study, we co-encapsulated a photosensitizer, chlorophyllin-copper complex, and CdSe/ZnS core-shell QDs as energy donors into biodegradable polymeric NPs composed of poly (lactide-co-glycolide) (PLGA). We investigated the morphological and photophysical properties of NPs, with special attention to the QD fluorescence properties changes upon encapsulation, then we studied the ability of this formulation to generate ROS in an aqueous environment and in living cells.…”
Section: Poly(lactide-co-glycolide) Nanoparticles Co-loaded With Chlomentioning
Photodynamic therapy (PDT) is an approach to treating cancer and involves light‐induced activation of a photosensitizer that triggers the formation of reactive oxygen species (ROS) in targeted cells and subsequent cell death. Examples of photosensitizers are porphyrins, including the natural compound chlorophyll. These molecules can be delivered alone or co‐formulated with an agent, such as quantum dots (QDs), that is able to excite them through a fluorescence resonance energy transfer (FRET)‐based mechanism. We encapsulated a chlorophyllin copper complex and CdSe/ZnS core‐shell QDs into biodegradable nanoparticles (NPs) composed of poly(lactide‐co‐glycolide) (PLGA), that allow modification with specific targeting ligands. When excited at 365 nm, FRET occurs between co‐encapsulated QDs and chlorophyllin to result in the formation of ROS. This chlorophyllin‐QD coformulation allows generation of ROS both in an aqueous environment and in cells, thus confirming the potential of this formulation in PDT.
“…6 Synthesis of gelatin nanoparticles has been done in different ways, most of which in the final stages of synthesis, it is necessary to use a combination of linker (cross linking agent) to stabilize the nanoparticles Fig. 7 Synthesis of gelatin nanoparticles by self-assembly method: in this method, hexanoic anhydride molecules are first attached to it through the lysine roots in collagen, resulting in the formation of an amphipathic structure that spontaneously Nanoparticles accumulate [ 96 ] …”
In this article, we will describe the properties of albumin and its biological functions, types of sources that can be used to produce albumin nanoparticles, methods of producing albumin nanoparticles, its therapeutic applications and the importance of albumin nanoparticles in the production of pharmaceutical formulations. In view of the increasing use of Abraxane and its approval for use in the treatment of several types of cancer and during the final stages of clinical trials for other cancers, to evaluate it and compare its effectiveness with conventional non formulations of chemotherapy Paclitaxel is paid. In this article, we will examine the role and importance of animal proteins in Nano medicine and the various benefits of these biomolecules for the preparation of drug delivery carriers and the characteristics of plant protein Nano carriers and protein Nano cages and their potentials in diagnosis and treatment. Finally, the advantages and disadvantages of protein nanoparticles are mentioned, as well as the methods of production of albumin nanoparticles, its therapeutic applications and the importance of albumin nanoparticles in the production of pharmaceutical formulations.
“…Entretanto, a maioria dos fotossensibilizadores de segunda geração, são altamente hidrofóbicos, apresentando baixa solubilidade em água, ou seja, em solventes fisiologicamente compatíveis. Uma vertente para solucionar esse problema de solubilidade em soluções aquosas causado por sua elevada hidrofobicidade, é a incorporação desses compostos bioativos em diferentes sistemas de liberação controlada, chamados de drug delivery systems (DDS) (CARVALHO et al, 2018).…”
RESUMO Nas últimas décadas, avanços no campo das ciências e tecnologias aplicadas têm emergido, com pesquisas na área inovadora e multidisciplinar conhecida como nanobiotecnologia, referindo-se às tecnologias em que a matéria é manipulada às escalas atômica e molecular para criar novos materiais com características funcionais diferentes dos materiais comuns. O desenvolvimento destas tecnologias para aplicação em sistemas biológicos almeja viabilizar a utilização prática desse conhecimento nas áreas da saúde. A Terapia Fotodinâmica (TFD) se configura como uma terapia promissora para o tratamento de diversas doenças, baseado em fotoprocesso onde um fotossensibilizador é ativado por luz visível, em um comprimento de onda adequado, na presença de oxigênio molecular. Essa combinação é responsável por gerar espécies reativas de oxigênio (EROs) e oxigênio singlete, levando a morte do tecido alvo devido a uma cascata de eventos biológicos. Porém, muitos dos fotossensibilizadores apresentam baixa solubilidade em meio fisiológico, deste modo, faz-se necessária a utilização de sistema de liberação controlada. Neste âmbito, destaca-se o processo de síntese das micropartículas precipitadas de carbonato de cálcio (CaCO3) de maneira controlada, buscando favorecer a nucleação e o crescimento de um determinado polimorfo de CaCO3. O objetivo do trabalho foi controlar os parâmetros na síntese das micropartículas, como supersaturação das soluções, agitação, adição de agentes estabilizantes, buscando as condições ideais para a obtenção das micropartículas esféricas na fase de vaterita (µ-CaCO3). A avaliação dos protocolos foi assistida por microscopia eletrônica de varredura a fim de determinar o processo que atingiu a estabilização da fase de vaterita.Palavras-chave: CaCO3. Vaterita. CMC. PSS. Estabilização de fase. ABSTRACTIn the last decades, advances in the field of applied sciences and technologies have emerged, with research in the innovative and multidisciplinary area known as nanobiotechnology, referring to technologies in which matter is manipulated at the atomic and molecular scales to create new materials with different functional characteristics. common materials. The development of these technologies for application in biological systems aims to enable the practical use of this knowledge in the areas of health. Photodynamic Therapy (PDT) is a promising therapy for the treatment of various diseases, based on a photoprocess where a photosensitizer is activated by visible light, at an appropriate wavelength, in the presence of molecular oxygen. This combination is responsible for generating reactive oxygen species (ROS) and singlet oxygen, leading to the death of the target tissue due to a cascade of biological events. However, many of the photosensitizers have low solubility in a physiological environment, so it is necessary to use a controlled release system. In this context, the process of synthesis of precipitated microparticles of calcium carbonate (CaCO3) stands out in a controlled manner, seeking to favor the nucleation and the growth of a specific CaCO3 polymorph. The objective of the work was to control the parameters in the synthesis of microparticles, such as supersaturation of solutions, agitation, addition of stabilizing agents, seeking the ideal conditions for obtaining spherical microparticles in the vaterite phase (µ-CaCO3). The evaluation of the protocols was assisted by scanning electron microscopy in order to determine the process that reached the stabilization of the vaterite phase.Keywords: CaCO3. Vaterite. CMC. PSS. Phase stabilization
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