Preparation of gelatin/genipin nanofibrous membrane for tympanic member repair

Li, Longfei; Zhang, Weizheng; Huang, Mengjia; Li, Jie; Chen, Jia; Zhou, Mi; He, Jian‐Guo

doi:10.1080/09205063.2018.1528519

Cited by 25 publications

(19 citation statements)

References 43 publications

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“…20 An aluminum foil collector plate was placed 10 cm from the tip of the needle. 20 Once the web of fibers was created, it was vacuum dried, and a 5 mM glutaraldehyde solution was used to crosslink the fibers, maintaining morphology and preventing dissolution. 20…”

Section: Resultsmentioning

confidence: 99%

“…19 Two in vitro studies were also identified, showing promise for gelatin/genipin nanofibrous scaffolds and poly(lactic-co-glycolic) acid and poly(ethylene oxide terephthalate)/poly(butylene terephthalate) nanofibrous scaffolds. 20,21 Both of these grafts were seeded with stem cells and showed appropriate mechanical characteristics for TM repair (Table 1). 20,21…”

Section: Resultsmentioning

confidence: 99%

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Applications of Electrospinning for Tissue Engineering in Otolaryngology

Heilingoetter

Smith

Malhotra

et al. 2020

Ann Otol Rhinol Laryngol

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Objective: In tissue engineering, biomaterials create a 3D scaffold for cell-to-cell adhesion, proliferation and tissue formation. Because of their similarity to extracellular matrix and architectural adaptability, nanofibers are of particular interest in tissue engineering. Electrospinning is a well-documented technique for nanofiber production for tissue engineering scaffolds. Here we present literature on the applications of electrospinning in the field of otolaryngology. Review Methods: A PubMed database search was performed to isolate articles published about applications of electrospun nanofibers for tissue engineering in otolaryngology. Study design, size, material tested, site of application within the head and neck, and outcomes were obtained for each study. Results: Almost all data on electrospinning in otolaryngology was published in the last 6 years (84%), highlighting its novelty. A total of 25 pre-clinical studies were identified: 9 in vitro studies, 5 in vivo animal studies, and 11 combination studies. Sites of application included: tracheal reconstruction (n = 16), tympanic membrane repair (n = 3), cranial nerve regeneration (n = 3), mastoid osteogenesis (n = 1) and ear/nose chondrogenesis (n = 2). Implications for Practice: Tissue engineering is a burgeoning field, with recent innovative applications in the field of otolaryngology. Electrospun nanofibers specifically have relevant applications in the field of otolaryngology, due in part to their similarity to native extracellular matrix, with emerging areas of interest being tympanic membrane repair, cranial nerve regeneration and tracheal reconstruction.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Applications of Electrospinning for Tissue Engineering in Otolaryngology

Heilingoetter

Smith

Malhotra

et al. 2020

Ann Otol Rhinol Laryngol

View full text Add to dashboard Cite

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“…A few studies in the past have attempted to replicate similar geometrical arrangements while reporting biofabricated eardrums [23,24,32]. However, the majority of them have not considered it as an important aspect for mimicking the mechanical, acoustical and biological properties of the native tissue [25,26,[28][29][30][31]33]. Interestingly, even the ones that did focus on recreating the appropriate anatomy, lacked consistency in their chosen geometries.…”

Section: Discussionmentioning

confidence: 99%

“…With the recent progress in tissue engineering, novel biomaterial-based TM scaffolds have emerged as a promising alternative to the conventional tissue grafts [23][24][25][26][27][28][29][30][31][32][33]. Biomimetic constructs aiming to replicate the precise architecture and mechano-acoustical properties of the human TM have been investigated [23,24]; however, the construction of a functional eardrum replacement is yet to be attained.…”

Section: Introductionmentioning

confidence: 99%

“…Among the current tissue engineering strategies, biofabrication has emerged as a powerful toolbox for creating three-dimensional (3D) tissue and organ constructs [38,39]. Several manufacturing techniques, such as, fused deposition modeling (FDM) [23,24], 3D bioprinting [25,26], selective laser sintering [27], and electrospinning (ES) [28][29][30][31][32][33] have been explored for the human eardrum regeneration. These technologies allow the creation of complex biomimetic scaffolds for steering cell activity, however, not many have considered the distinctive anatomy of the native TM.…”

Section: Introductionmentioning

confidence: 99%

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Mimicking the Human Tympanic Membrane: the Significance of Geometry

Anand

Stoppe

Lucena

et al. 2020

Preprint

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The human tympanic membrane (TM) captures sound waves reaching the outer ear from the environment and transforms them into mechanical motion. The successful transmission of these acoustic vibrations in varying frequency ranges is attributed to the structural architecture of the TM. However, a limited knowledge is available on the contribution of its discrete anatomical features, which is important to fabricate functional biomimetic TM replacements. This work synergizes theoretical and experimental approaches toward understanding the significance of geometry in tissue engineered TM scaffolds. Three test designs along with a plain control were chosen to decouple some of the dominant structural attributes, such as, the radial and circumferential alignment of the collagen fibrils. In silico models suggested a geometrical dependency of their mechanical and acoustical responses, where the presence of radially aligned fibers was observed to have a more prominent effect compared to their circumferential counterparts. Following which, a hybrid fabrication strategy combining electrospinning and additive manufacturing was optimized to manufacture hierarchical scaffolds within the dimensions of the native TM. The experimental characterizations conducted using macro-indentation and laser Doppler vibrometry were in line with the computational models. Finally, biological studies performed with human dermal fibroblasts and human mesenchymal stromal cells, revealed a favorable influence of scaffold hierarchy on cellular alignment and subsequent collagen deposition.Abstract FigureGraphical abstract.Schematic diagram illustrating the overall flowchart of the work. 3D: three-dimensional; ES: electrospinning; FDM: fused deposition modeling; TM: tympanic membrane.

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Mimicking the Human Tympanic Membrane: The Significance of Scaffold Geometry

Anand

Stoppe

Lucena

et al. 2021

Adv Healthcare Materials

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The human tympanic membrane (TM) captures sound waves from the environment and transforms them into mechanical motion. The successful transmission of these acoustic vibrations is attributed to the unique architecture of the TM. However, a limited knowledge is available on the contribution of its discrete anatomical features, which is important for fabricating functional TM replacements. This work synergizes theoretical and experimental approaches toward understanding the significance of geometry in tissue-engineered TM scaffolds. Three test designs along with a plain control are chosen to decouple some of the dominant structural elements, such as the radial and circumferential alignment of the collagen fibrils. In silico models suggest a geometrical dependency of their mechanical and acoustical responses, where the presence of radially aligned fibers is observed to have a more prominent effect compared to their circumferential counterparts. Following which, a hybrid fabrication strategy combining electrospinning and additive manufacturing has been optimized to manufacture biomimetic scaffolds within the dimensions of the native TM. The experimental characterizations conducted using macroindentation and laser Doppler vibrometry corroborate the computational findings. Finally, biological studies with human dermal fibroblasts and human mesenchymal stromal cells reveal a favorable influence of scaffold hierarchy on cellular alignment and subsequent collagen deposition.

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Preparation of gelatin/genipin nanofibrous membrane for tympanic member repair

Cited by 25 publications

References 43 publications

Applications of Electrospinning for Tissue Engineering in Otolaryngology

Applications of Electrospinning for Tissue Engineering in Otolaryngology

Mimicking the Human Tympanic Membrane: the Significance of Geometry

Mimicking the Human Tympanic Membrane: The Significance of Scaffold Geometry

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