Preparation of ethynyloestradiol‐4‐<sup>14</sup>C

Cooley, G.; Harris, I. A.

doi:10.1002/jlcr.2590050103

Cited by 3 publications

(1 citation statement)

References 8 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[4-14C]Ethynyloestradiol was prepared as described by Cooley & Harris (1969); it had a specific activity of 40 jud/rag and was more than 95 % pure as shown by paper chromatography.…”

Section: Methodsmentioning

confidence: 99%

Localization of Ethynyloestradiol in the Reproductive Tract of Women

Reed¹,

Fotherby²,

Steele³

1973

Journal of Endocrinology

View full text Add to dashboard Cite

4-14C]Ethynyloestradiol was administered to women at varying times before hysterectomy. At operation, samples of blood, adipose tissue and tissue from the reproductive tract were obtained. All tissues examined took up radioactivity, the concentration at 1 h varying from 0\m=.\22 to 0\m=.\4% of the dose per 100 g of tissue except for stroma and endometrium which showed high levels (0\m=.\68% and 0\m=.\60%,respectively). By 24 h the amount of radioactivity in the tissues had decreased markedly. Mean values for the amount of radioactivity present in total tissues at 1 h were: uterus, 0\m=.\9%; adipose tissue, 28\m=.\2%;blood, 8\m=.\8%.By 24 h these values had declined to 0\m=.\2%,6\m=.\8% and 1\m=.\6%,respectively. In adipose tissue almost all the radioactivity was present in a freely extractable state whereas in myometrium and cervix about 20 % of the radioactivity was in a conjugated form. In plasma, more than 80% of the metabolites were conjugated, mainly as sulphates. The major metabolite detected in the uterus was ethynyloestradiol itself and most of the radioactivity in the myometrium was associated with the nuclear fraction. In one pregnant subject studied, radioactivity crossed the placenta and small amounts of the dose were found in the foetus.

show abstract

“…[4-14C]Ethynyloestradiol was prepared as described by Cooley & Harris (1969); it had a specific activity of 40 jud/rag and was more than 95 % pure as shown by paper chromatography.…”

Section: Methodsmentioning

confidence: 99%

Localization of Ethynyloestradiol in the Reproductive Tract of Women

Reed¹,

Fotherby²,

Steele³

1973

Journal of Endocrinology

View full text Add to dashboard Cite

show abstract

Synthesis of 17α ‐ethynylestradiol‐6, 7‐³H and 17α‐ethynylestradiol‐6,7‐³H, 3‐cyclopentyl‐1‐¹⁴C ether

Merrill

Vernice

1970

J. Labelled Cpd. Radiopharm.

View full text Add to dashboard Cite

show abstract

Covalent binding of ethinylestradiol and estrone to rat liver DNA in vivo

Jaggi¹,

Lutz²,

Schlatter³

1978

Chemico-Biological Interactions

View full text Add to dashboard Cite

SUMMARYThecovalent bindingof [6, H]ethinylestradiol (EE)and [6,7-3 H]estrone (E) to liver DNA of 200 g female ratswas measured 8 h after the administration of 80 J..Lg (9.2 mCi) estrogen by gavage. The binding is 1.5 for EE and 1.1 for E, expressedas binding to DNA/dose, in units of ~mol hormonefmol DNA phosphate/mmole honnone/kg body wt. It is in the same order of magnitude as for benzene and about 10 000 tim es below the binding of typical liver carcinogens, such as aflatoxin B 1 or N,N-dimethylnitrosamine.

show abstract

Preparation of ethynyloestradiol‐4‐¹⁴C

Abstract: SUMMARYOe~trone-4-C in dimethyl sulphoxide solutioii has been treated with lithium acetylide (ethylene diamine complex) to give ethynyloestradi01-4-C. INTRODUCTION.

Cited by 3 publications

References 8 publications

Localization of Ethynyloestradiol in the Reproductive Tract of Women

Localization of Ethynyloestradiol in the Reproductive Tract of Women

Synthesis of 17α ‐ethynylestradiol‐6, 7‐³H and 17α‐ethynylestradiol‐6,7‐³H, 3‐cyclopentyl‐1‐¹⁴C ether

Covalent binding of ethinylestradiol and estrone to rat liver DNA in vivo

Contact Info

Product

Resources

About

Preparation of ethynyloestradiol‐4‐14C

Abstract: SUMMARYOe~trone-4-~~C in dimethyl sulphoxide solutioii has been treated with lithium acetylide (ethylene diamine complex) to give ethynyloestradi01-4-~~C. INTRODUCTION.

Cited by 3 publications

References 8 publications

Localization of Ethynyloestradiol in the Reproductive Tract of Women

Localization of Ethynyloestradiol in the Reproductive Tract of Women

Synthesis of 17α ‐ethynylestradiol‐6, 7‐3H and 17α‐ethynylestradiol‐6,7‐3H, 3‐cyclopentyl‐1‐14C ether

Covalent binding of ethinylestradiol and estrone to rat liver DNA in vivo

Contact Info

Product

Resources

About

Preparation of ethynyloestradiol‐4‐¹⁴C

Abstract: SUMMARYOe~trone-4-C in dimethyl sulphoxide solutioii has been treated with lithium acetylide (ethylene diamine complex) to give ethynyloestradi01-4-C. INTRODUCTION.

Synthesis of 17α ‐ethynylestradiol‐6, 7‐³H and 17α‐ethynylestradiol‐6,7‐³H, 3‐cyclopentyl‐1‐¹⁴C ether