Preparation of epidermal growth factor (EGF) conjugated iron oxide nanoparticles and their internalization into colon cancer cells

Creixell, Mar; Herrera, Adriana; Ayala, Vanessa; Latorre-Esteves, Magda; Pérez-Torres, Marianela; Torres‐Lugo, Madeline; Rinaldi, Carlos

doi:10.1016/j.jmmm.2010.02.019

Cited by 38 publications

(18 citation statements)

References 33 publications

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“…Surface areas are commonly decorated with coatings that are intended for two purposes: to enhance the biocompatibility of the nanoparticle and to gain bioactivity, ie, to become an active player in the biological niche of interest. This bioactivity has been exploited for cell targeting, 2,3 cargo payload (drug and gene delivery), [4][5][6] contrast agents, 7 or a combination of these, giving multifunctionality to a nanostructure. [8][9][10] Magnetic nanoparticles (MNPs) can be remotely manipulated by magnetic fields.…”

Section: Introductionmentioning

confidence: 99%

Non-chemotoxic induction of cancer cell death using magnetic nanowires

Contreras¹,

Sougrat²,

Zaher³

et al. 2015

IJN

101

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In this paper, we show that magnetic nanowires with weak magnetic fields and low frequencies can induce cell death via a mechanism that does not involve heat production. We incubated colon cancer cells with two concentrations (2.4 and 12 μg/mL) of nickel nanowires that were 35 nm in diameter and exposed the cells and nanowires to an alternating magnetic field (0.5 mT and 1 Hz or 1 kHz) for 10 or 30 minutes. This low-power field exerted a force on the magnetic nanowires, causing a mechanical disturbance to the cells. Transmission electron microscopy images showed that the nanostructures were internalized into the cells within 1 hour of incubation. Cell viability studies showed that the magnetic field and the nanowires separately had minor deleterious effects on the cells; however, when combined, the magnetic field and nanowires caused the cell viability values to drop by up to 39%, depending on the strength of the magnetic field and the concentration of the nanowires. Cell membrane leakage experiments indicated membrane leakage of 20%, suggesting that cell death mechanisms induced by the nanowires and magnetic field involve some cell membrane rupture. Results suggest that magnetic nanowires can kill cancer cells. The proposed process requires simple and low-cost equipment with exposure to only very weak magnetic fields for short time periods.

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Section: Introductionmentioning

confidence: 99%

Non-chemotoxic induction of cancer cell death using magnetic nanowires

Contreras¹,

Sougrat²,

Zaher³

et al. 2015

IJN

101

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“…However, the potential physiological activation and stimulation of cancer growth has hindered the use of EGF as targeting peptide in drug delivery systems, which is dependent on the release of the anticancer drug [7]. When conjugated to metallic nanoparticles, EGF promotes a rapid internalization into cancer cells [8], and cancer destruction can be achieved by injecting the light-absorbing nanoparticles locally and applying the laser-mediated hyperthermia directly into the tumour. Therefore, it is our aim to use EGF-conjugated HAOA-coated gold nanoparticles with plasmon absorption band located in the near-infrared (NIR) range (i.e., 650–900 nm), for photothermal therapy and local hyperthermia, without damage to the surrounding tissues [9].…”

Section: Introductionmentioning

confidence: 99%

EGF Functionalized Polymer-Coated Gold Nanoparticles Promote EGF Photostability and EGFR Internalization for Photothermal Therapy

et al. 2016

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The application of functionalized nanocarriers on photothermal therapy for cancer ablation has wide interest. The success of this application depends on the therapeutic efficiency and biocompatibility of the system, but also on the stability and biorecognition of the conjugated protein. This study aims at investigating the hypothesis that EGF functionalized polymer-coated gold nanoparticles promote EGF photostability and EGFR internalization, making these conjugated particles suitable for photothermal therapy. The conjugated gold nanoparticles (100–200 nm) showed a plasmon absorption band located within the near-infrared range (650–900 nm), optimal for photothermal therapy applications. The effects of temperature, of polymer-coated gold nanoparticles and of UVB light (295nm) on the fluorescence properties of EGF have been investigated with steady-state and time-resolved fluorescence spectroscopy. The fluorescence properties of EGF, including the formation of Trp and Tyr photoproducts, is modulated by temperature and by the intensity of the excitation light. The presence of polymeric-coated gold nanoparticles reduced or even avoided the formation of Trp and Tyr photoproducts when EGF is exposed to UVB light, protecting this way the structure and function of EGF. Cytotoxicity studies of conjugated nanoparticles carried out in normal-like human keratinocytes showed small, concentration dependent decreases in cell viability (0–25%). Moreover, conjugated nanoparticles could activate and induce the internalization of overexpressed Epidermal Growth Factor Receptor in human lung carcinoma cells. In conclusion, the gold nanoparticles conjugated with Epidermal Growth Factor and coated with biopolymers developed in this work, show a potential application for near infrared photothermal therapy, which may efficiently destroy solid tumours, reducing the damage of the healthy tissue.

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“…Epidermal growth factor (EGF)-containing polyamidoamine dendrimers can localize within cells that express the EGFR in a receptor-dependent manner, whereas uptake into cells lacking the receptor was low (Yuan et al 2010). EGF-conjugated iron oxide MNPs were reported to accumulate very fast in both small vesicles and large circular endocytic structures through clathrin-dependent and clathrinindependent receptors mediated endocytosis pathways (Creixell et al 2010). Integrins are overexpressed in tumor cells compared to normal endothelial cells.…”

Section: Targeted Drug Deliverymentioning

confidence: 99%

Nanoscale Materials in Targeted Drug Delivery

Kumari

Singla

Guliani

et al. 2016

Nanoscale Materials in Targeted Drug Delivery, Theragnosis and Tissue Regeneration

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Nanoscale materials (NSMs) are gaining attention due to their small size and unique physiochemical properties. Different approaches are used for the synthesis of NSMs. NSMs cannot be visualized by ordinary instruments. Instruments with high resolution are required for their characterisation. NSMs offer enhanced activity and specificity to encapsulate drugs. NSM are used as vehicles for site specific delivery of drugs inside the body. In this chapter, approaches for the synthesis of NSMs, and characterisation techniques for NSMs like SEM, TEM, AFM, DLS, XRD, and FTIR have been explained briefly. In addition, this chapter also covers zero dimensional one dimensional, two dimensional and three dimensional NSMs briefly. Detailed information about approaches of targeted delivery like passive and active have been covered in this chapter. NSMs like polymeric nanoparticles, metallic nanoparticles, liposomes, quantum dots, polymeric micelles, carbon nanotubes, dendrimers, and magnetic nanoparticles used in targeted drug delivery have also been explained in this chapter.

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Preparation of epidermal growth factor (EGF) conjugated iron oxide nanoparticles and their internalization into colon cancer cells

Cited by 38 publications

References 33 publications

Non-chemotoxic induction of cancer cell death using magnetic nanowires

Non-chemotoxic induction of cancer cell death using magnetic nanowires

EGF Functionalized Polymer-Coated Gold Nanoparticles Promote EGF Photostability and EGFR Internalization for Photothermal Therapy

Nanoscale Materials in Targeted Drug Delivery

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