Preparation of endostatin-loaded chitosan nanoparticles and evaluation of the antitumor effect of such nanoparticles on the Lewis lung cancer model

Ding, Ruilin; Xie, Fang; Hu, Yue; Fu, Shaozhi; Wu, Jingbo; Fan, Juan; He, Wenfeng; He, Yu xiao; Yang, Linglin; Lin, Sheng; Wen, Qinglian

doi:10.1080/10717544.2016.1247927

Cited by 33 publications

(16 citation statements)

References 37 publications

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“…The recombinant human endostatin, Endostar, was provided by Shandong Simcere Medgenn Bio-pharmaceutical Co. Ltd. (Yantai, Shandong, China) and stored at 4 °C until required. The doses and the schedules of administration of reference drugs were based on previous studies [ 13 , 14 ].…”

Section: Methodsmentioning

confidence: 99%

Enhanced antitumor and anti-angiogenic effects of metronomic Vinorelbine combined with Endostar on Lewis lung carcinoma

Qin¹,

Zhang

Zhu

et al. 2018

BMC Cancer

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BackgroundConventional chemotherapy is commonly used to treat non-small cell lung cancer (NSCLC) however it increases therapeutic resistance. In contrast, metronomic chemotherapy (MET) is based on frequent drug administration at lower doses, resulting in inhibition of neovascularization and induction of tumor dormancy. This study aims to evaluate the inhibitory effects, adverse events, and potential mechanisms of MET Vinorelbine (NVB) combined with an angiogenesis inhibitor (Endostar).MethodsCirculating endothelial progenitor cells (CEPs), apoptosis rate, expression of CD31, vascular endothelial growth factor (VEGF), hypoxia inducible factor-1 (HIF-1α) were determined using flow cytometry, western blot analysis, immunofluorescence staining and Enzyme-linked immunosorbent assay (ELISA) analysis. And some animals were also observed using micro fluorine-18-deoxyglucose PET/computed tomography (18F-FDG PET/CT) to identify changes by comparing SUVmax values. In addition, white blood cell (WBC) counts and H&E-stained sections of liver, lungs, kidney, and heart were performed in order to monitor toxicity assessments.ResultsWe found that treatment with MET NVB + Endo was most effective in inhibiting tumor growth, decreasing expression of CD31, VEGF, HIF-1α, and CEPs, and reducing side effects, inducing apoptosis, such as expression of Bcl-2, Bax and caspase-3. Administration with a maximum tolerated dose of NVB combined with Endostar (MTD NVB + Endo) demonstrated similar anti-tumor effects, including changes in glucose metabolism with micro fluorine-18-deoxyglucose PET/computed tomography (18F-FDG PET/CT) imaging, however angiogenesis was not inhibited. Compared with either agent alone, the combination of drugs resulted in better anti-tumor effects.ConclusionThese results indicated that MET NVB combined with Endo significantly enhanced anti-tumor and anti-angiogenic responses without overt toxicity in a xenograft model of human lung cancer.

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Section: Methodsmentioning

confidence: 99%

Enhanced antitumor and anti-angiogenic effects of metronomic Vinorelbine combined with Endostar on Lewis lung carcinoma

Qin¹,

Zhang

Zhu

et al. 2018

BMC Cancer

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“…Chitosan is a non-toxic material widely used as nanoparticle-forming polymer. 11 Therefore, this study designed to prepare nano chitosan-Annona squamosa leaf extract to enhance their bioactivities as the anticancer agent on HeLa cells to evaluate the caspase expression as one of apoptosis marker of cells.…”

Section: Introductionmentioning

confidence: 99%

Apoptosis of HeLa Cells via Caspase-3 Expression Induced by Chitosan-Based Nanoparticles of Annona squamosa Leaf Extract: In vitro Study

Fadholly¹,

Ansori²,

Proboningrat³

et al. 2020

IJPER

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Background: Annona squamosa is reported has a significant cytotoxic activity in some cancer cells. Objectives: Thus, this study aim to investigate Annona squamosa leaf extract induced by chitosan nanoparticles (nano-ASLE) to enhance their biological activity as anticancer agent on HeLa cells. Methods: Nano-ASLE (50, 100, 200, 400 µg/mL in DMSO) given on HeLa cells to determined IC 50 value by MTT assay. Then, it was devided into three groups as follow IC 50 , 2IC 50 , 4IC 50 continued with analysis of caspase-3 expression. Results: The present study demonstrated that nano-ASLE can surpress HeLa cells proliferation with the IC 50 value of 344.48 µg/mL and rapid enhancement of caspase-3 activity has the mean score of 65.3 cell expression and the lowest score shows 45.3 cell expression. Conclusion: Nano-ASLE lead to HeLa cell death via the mitochondrial pathway on caspase-3 expression. In addition, the further studies are needed to obtain the loading efficiency, release of drug concentration and in vivo study of nano-ASLE to suppress HeLa cells.

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“…In the present study, the combination of pH – and temperature – responsive properties were generated by means of graft copolymerization of chitosan (CS) with poly( N -isopropylacrylamide) (PNIPAM) so as to carry out these dual-responsive functions concurrently. As is well known (Chan et al., 2007 ), CS (2-amino-2-deoxy-(1 → 4)-β- d -glucosamine), which is obtained from chitin by deacetylation, is considered to be one of the most widely distributed cationic biopolymers (Ding et al., 2017 ). It has good biocompatibility and biodegradability and has been used extensively in the preparation of safe biomaterials.…”

Section: Introductionmentioning

confidence: 99%

l-Peptide functionalized dual-responsive nanoparticles for controlled paclitaxel release and enhanced apoptosis in breast cancer cells

Niu

Bremner

et al. 2018

Drug Delivery

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Nanoparticles and macromolecular carriers have been widely used to increase the efficacy of chemotherapeutics, largely through passive accumulation provided by their enhanced permeability and retention effect. However, the therapeutic efficacy of nanoscale anticancer drug delivery systems is severely truncated by their low tumor-targetability and inefficient drug release at the target site. Here, the design and development of novel l-peptide functionalized dual-responsive nanoparticles (l-CS-g-PNIPAM-PTX) for active targeting and effective treatment of GRP78-overexpressing human breast cancer in vitro and in vivo are reported. l-CS-g-PNIPAM-PTX NPs have a relative high drug loading (13.5%) and excellent encapsulation efficiency (74.3%) and an average diameter of 275 nm. The release of PTX is slow at pH 7.4 and 25 °C but greatly accelerated at pH 5.0 and 37 °C. MTT assays and confocal experiments showed that the l-CS-g-PNIPAM-PTX NPs possessed high targetability and antitumor activity toward GRP78 overexpressing MDA-MB-231 human breast cancer cells. As expected, l-CS-g-PNIPAM-PTX NPs could effectively treat mice bearing MDA-MB-231 human breast tumor xenografts with little side effects, resulting in complete inhibition of tumor growth and a high survival rate over an experimental period of 60 days. These results indicate that l-peptide-functionalized acid – and thermally activated – PTX prodrug NPs have a great potential for targeted chemotherapy in breast cancer.

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Preparation of endostatin-loaded chitosan nanoparticles and evaluation of the antitumor effect of such nanoparticles on the Lewis lung cancer model

Cited by 33 publications

References 37 publications

Enhanced antitumor and anti-angiogenic effects of metronomic Vinorelbine combined with Endostar on Lewis lung carcinoma

Enhanced antitumor and anti-angiogenic effects of metronomic Vinorelbine combined with Endostar on Lewis lung carcinoma

Apoptosis of HeLa Cells via Caspase-3 Expression Induced by Chitosan-Based Nanoparticles of Annona squamosa Leaf Extract: In vitro Study

l-Peptide functionalized dual-responsive nanoparticles for controlled paclitaxel release and enhanced apoptosis in breast cancer cells

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