Preparation of enantiomerically enriched (S)-ethyl 3-hydroxy 4,4,4-trifluorobutanoate using whole cells of Candida parapsilosis ATCC 7330

Direct observation of redox reactions in Candida parapsilosis ATCC 7330 by Confocal microscopic studies

“…Even though cells harvested at 14 h produced the same ketone at less conversion (38%), these cells were used to visualise the site of biotransformation. For several other substrates reported earlier from our lab, the 14 h harvested cells produced the desired optically pure products with maximum ee (up to >99%) 1 2 3 6 7 26 29 30 33 34 35 .…”

“…The TLC indicated no trace of product 2a formation even after 48 h. The next experiment was carried out ( Case Ib; Fig. 1 ) where the alcohol 2a was the substrate for biocatalytic oxidation (Tris-HCl buffer, pH 8.5, 10 mM) with acetone as cosubstrate and ethanol as cosolvent 26 . The progress of the reaction as monitored by TLC did not show aldehyde formation and the substrate remained unreacted even after 48 h.…”

“…This substrate 1b could not be used for further studies and in any case the product alcohol is non-fluorescent. The racemic alcohol 2b ( Case IIb ) was used as a substrate (substrate concentration: 2.8 mM) for deracemisation using the earlier optimised conditions 26 and the reaction was carried out for 1 h. The reaction was expected to proceed via stereoinversion mechanism like other aryl racemic alcohols carried out using this biocatalyst 4 5 6 . The progress of the reaction was monitored using TLC and the intermediate formation of the fluorescent ketone 1b was observed along with the alcohol.…”

Enantio- & chemo-selective preparation of enantiomerically enriched aliphatic nitro alcohols using Candida parapsilosis ATCC 7330

“…The concentration of allyl alcohol used was as per our earlier report. 51 Allyl alcohol (8.6 mM) or allyl bromide (4.13 mM) was added to the cell suspension (separately) and incubated for 1 h. The product 2a 0 obtained aer allyl alcohol treatment showed a marginal increase in ee of up to 50%, giving the (R)-alcohol with a marked decrease in conversion (74%) unlike the untreated cells which produced (R)-alcohol with 42% ee and 95% conversion (Scheme 2). The marginal increase in ee of (R)-2a 0 could be attributed by the selective inhibition of (S)-specic enzymes present in the biocatalyst.…”

Chemoenzymatic Synthesis of an Enantiomerically Enriched Bicyclic Carbocycle Using <i>Candida parapsilosis</i> ATCC 7330 Mediated Enantioselective Hydrolysis

“…The versatility of the biocatalyst Candida parapsilosis ATCC 7330 was established in our lab. This yeast is used for the preparation of a variety of enantiopure compounds by deracemization [36]- [39], asymmetric reduction [40] [41] and oxidative kinetic resolution [42].…”