Preparation of egg yolk antibodies against BoNT/B and their passive protection in mouse models

You, Zherong; Yang, Hao; Xin, Wenwen; Kang, Lin; Gao, Shan; Wang, Junhong; Zhang, Tao; Wang, Jinglin

doi:10.4161/hv.29433

Cited by 6 publications

(5 citation statements)

References 20 publications

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“…However, other studies have also found that certain proteins, such as natto kinase and earthworm kinase, could be absorbed into the blood through the intestinal epithelium of mammals as intact molecules. These proteins were absorbed into the circulation from mouse gut as intact molecules [7,19,20]. Our study confirmed these findings, although the detailed processes remain unclear.…”

Section: Discussionsupporting

confidence: 85%

“…The United States Department of Agriculture reported that the mastitis symptoms of dairy cows were obviously reduced after rearing with IgY anti‐cow mastitis virus for 3 weeks at a dose of 200 mg·kg −1 ·day −1 . The intragastric administration of anti‐heavy chain of botulinum neurotoxin (BHC) IgY can prevent a fatal attack of tetanus toxin, and the intraperitoneal injection of anti‐BHC IgY could play a protective role in mice . Our previous studies have also shown that the oral administration of anti‐LPS IgY could reduce the mortality rate and levels of inflammatory factors in the blood of mice attacked by endotoxin .…”

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confidence: 99%

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Intact anti‐LPS IgY is found in the blood after intragastric administration in mice

et al. 2019

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Severe burn injury and cirrhosis often cause the translocation of bacterial endotoxins into blood, leading to systemic damage and even death. Our previous studies have shown that anti‐lipopolysaccharide egg yolk antibody (anti‐LPS IgY) can neutralize bacterial endotoxins in vitro and in vivo effectively, thereby reducing endotoxin damage. Whether anti‐LPS IgY can be absorbed into the blood through the intestinal barrier and neutralize endotoxins in circulation remains unclear. In this study, we used in vivo small animal imaging techniques, protein purification, molecular biology, and mass spectrometry to show that intragastrically administered anti‐LPS IgY is detected in the blood of mice as an intact molecule and has the capacity to bind to LPS. Immunohistochemical analysis confirmed that anti‐LPS IgY is associated with the intestinal mucosa of mice. However, the route of absorption of this large protein molecule was not determined. This study suggests that anti‐LPS IgY can be absorbed into the circulation, with the same molecular mass as purified anti‐LPS IgY as a macromolecular protein, suggesting a new strategy for the prevention of damage caused by endotoxins.

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Section: Discussionsupporting

confidence: 85%

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confidence: 99%

Intact anti‐LPS IgY is found in the blood after intragastric administration in mice

et al. 2019

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“…In addition, they could be combined with traditional immunization procedures due its compatibility with current antivenoms manufacturing. In chickens, the use of innovative immunogens such as synthetic peptides (Egea et al, 2018;Guevarra et al, 2012;Moreno et al, 2016), recombinant toxins (Hirai et al, 2010;Mudili et al, 2015;Parma et al, 2012;Z. You et al, 2014) or DNA strings (Kazimierczuk et al, 2005;Witkowski et al, 2009) have demonstrated the production of specific antibodies with high titers, so they also should be explored to improve the production of IgY-based antivenoms.…”

Section: Accepted Manuscriptmentioning

confidence: 99%

IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy

Leiva

Cangelosi

Mariconda

et al. 2019

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“…Serotypically, this deadly toxin includes seven distinct types from BoNT/A to BoNT/G and possesses the metallopeptidase activity specific for the soluble N -ethylmaleimide-sensitive factor activating protein receptor (SNARE) complex. It could block the neurotransmitter release of the intoxicated peripheral neurons and cause death in consequence of blockade of respiratory function in vertebrates [1–4]. Among the seven serotypes, BoNT/A and B are commonly associated with human botulism, but the former causes more severe consequences than the latter [5].…”

Section: Introductionmentioning

confidence: 99%

An Ultrasensitive Gold Nanoparticle-based Lateral Flow Test for the Detection of Active Botulinum Neurotoxin Type A

et al. 2017

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Botulism is a severe and potentially lethal paralytic disease caused by several botulinum neurotoxin-producing Clostridia spp. In China, the majority of the cases caused by botulism were from less-developed rural areas. Here, we designed specific substrate peptides and reconfigured gold nanoparticle-based lateral flow test strip (LFTS) to develop an endopeptidase-based lateral flow assay for the diagnosis of botulism. We performed this lateral flow assay on botulinum neurotoxin-spiked human serum samples. The as-prepared LFTS had excellent performance in the detection of botulinum neurotoxin using only 1 μL of simulated serum, and its sensitivity and specificity were comparable to that of mouse lethality assay. Moreover, the assay takes only half a day and does not require highly trained laboratory staff, specialized facility, or equipment. Finally, our LFTS can be potentially extended to other serotypes of BoNTs by designing specific substrate peptides against the different types of BoNTs. Overall, we demonstrate a strategy by which LFTS and endopeptidase activity assays can be integrated to achieve facile and economic diagnosis of botulism in resource-limited settings.

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Preparation of egg yolk antibodies against BoNT/B and their passive protection in mouse models

Cited by 6 publications

References 20 publications

Intact anti‐LPS IgY is found in the blood after intragastric administration in mice

Intact anti‐LPS IgY is found in the blood after intragastric administration in mice

IgY-based antivenom against Bothrops alternatus: Production and neutralization efficacy

An Ultrasensitive Gold Nanoparticle-based Lateral Flow Test for the Detection of Active Botulinum Neurotoxin Type A

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