Preparation of disaggregated mouse brain cultures, and observations on early neurite and axon formation therein

Greenham, Leighton W.; Hill, Terry; Moss, C. A.; Peacock, D.B.

doi:10.1016/0014-4827(74)90408-x

Cited by 10 publications

(1 citation statement)

References 25 publications

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“…The formation of fibre bundles in insect nervous explant cultures has been reported previously (Levi-Montalcini &Chen, 1971;Provine et al, 1976) although in these situations the neurites were always associated with glial processes. However, certain mammalian explant cultures do produce neurite bridges and their formation is encouraged by nerve growth factor (Greenham et al, 1974).…”

Section: Discussionmentioning

confidence: 99%

Fine structure ofPeriplaneta americana neurons in long-term culture

1982

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A new culture technique is described for dissociated ganglia of embryos of Periplaneta americana. Neurons obtained by the mechanical dissociation of ganglia from 23-26 day old embryos were grown for seven days in a combination of Schneider's Drosophila Medium and Eagle's Basal Medium and then transferred to a combination of Leibovitz's L-15 Medium and Yunker's Modified Grace's Medium supplemented with ecdysone. Neurons cultured in this way survived for periods in excess of one month. The cells had a typically neuronal ultrastructure and produced axonal processes that associated to form fibre bundles. Within these processes neurosecretory granules, dense-core vesicles and clear vesicles were found. In some instances these vesicles aggregated at points of contact between fibres giving the appearance of presynaptic endings. These cultures would appear to be suitable for pharmacological studies of the insect nervous system.

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Section: Discussionmentioning

confidence: 99%

Fine structure ofPeriplaneta americana neurons in long-term culture

1982

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SV40 transformation of mouse brain cells: Critical role of gene A in maintenance of the transformed phenotype

Anderson

Martin

1976

Journal Cellular Physiology

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Brain cells derived from the NIH Swiss mouse strain have been established in tissue culture. Astrocytic neuroglial cells, identified by morphology and staining properties, predominate. The brain cell culture was successfully transformed with SV40 wild type virus and with a representative early (A239) and late (C219) mutant. When subjected to growth analysis the A239 transformant displayed selective loss of six characteristics of the transformed phenotype at the restrictive temperature (40.5 degrees C): doubling time, saturation density, ability to grow in low serum, efficiency of growth on plastic and on normal brain cell layers, and cloning in soft agar. Temperature shift experiment demonstrated the reversibility of the differences in saturation density. T-antigen was expressed at both temperatures. Alteration in uptake of 2-deoxyglucose was not a characteristic of the transformed phenotype. The cell lines may be utility in brain culture work and in studies on the mechanism of SV40 transformation.

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Nerve Growth Factor

Arnason¹,

Young²

1977

Brain Fetal and Infant

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Preparation of disaggregated mouse brain cultures, and observations on early neurite and axon formation therein

Cited by 10 publications

References 25 publications

Fine structure ofPeriplaneta americana neurons in long-term culture

Fine structure ofPeriplaneta americana neurons in long-term culture

SV40 transformation of mouse brain cells: Critical role of gene A in maintenance of the transformed phenotype

Nerve Growth Factor

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