Preparation of deuterated abscisic acid metabolites for use in mass spectrometry and feeding studies

Zaharia, L. Irina; Gałka, Marek; Ambrose, Stephen J.; Abrams, Suzanne R.

doi:10.1002/jlcr.939

Cited by 55 publications

(41 citation statements)

References 33 publications

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“…This is particularly important for preparative purposes, since biotransformation of either (+) or (-)-ABA in Zea mays L. cv. Black Mexican Sweet maize suspension cultures, an important source for obtaining some of the ABA metabolites (Zaharia et al, 2005b) did not yield quantity amounts of ABA-GE (Balsevich et al, 1994).…”

Section: Aba Metabolism In L Fendleri Suspension Cell Culturementioning

confidence: 99%

Abscisic acid metabolism and lipid accumulation of a cell suspension culture of Lesquerella fendleri

Kharenko

Zaharia

Giblin

et al. 2010

Plant Cell Tiss Organ Cult

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Lesquerella fendleri (commonly known as "Fendler's bladderpod" or "yellowtop") is a member of the Brassicaceae and is an important seed oil-producing plant. The lipid profile of L. fendleri seed indicates potential for producing a high quality replacement for castor oil. In this work, characterization of the lipid content of a suspension cell culture, derived from seedlings of L. fendleri, is provided. Under the described suspension cell culture conditions, 16:0, 18:1 9, 18:2 9, 12 and 18:3 9, 12, 15 fatty acids were found to accumulate in the cells, while 16:0, 26:0 and 28:0 fatty acids were predominant in the culture medium. Subsequently, the effect of application of abscisic acid (ABA), which modulates lipid accumulation, was assessed.

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Section: Aba Metabolism In L Fendleri Suspension Cell Culturementioning

confidence: 99%

Abscisic acid metabolism and lipid accumulation of a cell suspension culture of Lesquerella fendleri

Kharenko

Zaharia

Giblin

et al. 2010

Plant Cell Tiss Organ Cult

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“…The procedure for the quantification of ABA phytohormones and metabolites using deuterium-labeled internal standards (synthesized as described by Abrams et al [2003] and Zaharia et al [2005a]) has been presented in detail elsewhere (Ross et al, 2004;Owen et al, 2009). The mass spectrometer was set to collect data in multiple reaction monitoring mode controlled by MassLynx version 4.1 (Waters).…”

Section: Quantification Of Aba and Its Catabolitesmentioning

confidence: 99%

Gene Expression and Metabolite Profiling of Developing Highbush Blueberry Fruit Indicates Transcriptional Regulation of Flavonoid Metabolism and Activation of Abscisic Acid Metabolism

Zifkin

Jin²,

Ozga³

et al. 2011

Plant Physiology

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Highbush blueberry (Vaccinium corymbosum) fruits contain substantial quantities of flavonoids, which are implicated in a wide range of health benefits. Although the flavonoid constituents of ripe blueberries are known, the molecular genetics underlying their biosynthesis, localization, and changes that occur during development have not been investigated. Two expressed sequence tag libraries from ripening blueberry fruit were constructed as a resource for gene identification and quantitative realtime reverse transcription-polymerase chain reaction primer design. Gene expression profiling by quantitative real-time reverse transcription-polymerase chain reaction showed that flavonoid biosynthetic transcript abundance followed a tightly regulated biphasic pattern, and transcript profiles were consistent with the abundance of the three major classes of flavonoids. Proanthocyanidins (PAs) and corresponding biosynthetic transcripts encoding anthocyanidin reductase and leucoanthocyanidin reductase were most concentrated in young fruit and localized predominantly to the inner fruit tissue containing the seeds and placentae. Mean PA polymer length was seven to 8.5 subunits, linked predominantly via B-type linkages, and was relatively constant throughout development. Flavonol accumulation and localization patterns were similar to those of the PAs, and the B-ring hydroxylation pattern of both was correlated with flavonoid-3#-hydroxylase transcript abundance. By contrast, anthocyanins accumulated late in maturation, which coincided with a peak in flavonoid-3-O-glycosyltransferase and flavonoid-3#5#-hydroxylase transcripts. Transcripts of VcMYBPA1, which likely encodes an R2R3-MYB transcriptional regulator of PA synthesis, were prominent in both phases of development. Furthermore, the initiation of ripening was accompanied by a substantial rise in abscisic acid, a growth regulator that may be an important component of the ripening process and contribute to the regulation of blueberry flavonoid biosynthesis.Highbush blueberry (Vaccinium corymbosum; Ericaceae) is one of the most economically important fruit crops in North America. Blueberry fruit have been the focus of much recent attention due to numerous reports of their positive effects on human health. These benefits are generally attributed to high levels of polyphenolics, in particular the flavonoids (Rasmussen et al., 2005). Highbush blueberries have one of the highest in vitro antioxidant capacities of any fruit or vegetable (Prior and Gu, 2005;Wu et al., 2006). The major health benefits linked to the consumption of blueberries include a reduced risk for cardiovascular (Basu et al., 2010) and neurodegenerative (Neto, 2007) diseases. Furthermore, experiments on rodents suggest that blueberry extracts may also prevent cancer, slow tumor growth, and reverse cognitive and behavioral deficits related to stroke and aging (Lau et al., 2005;Gordillo et al., 2009).The three common types of flavonoids that accumulate in blueberry fruit are the flavonols, anthocya-

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“…The resulting 8'-hydroxyABA can be rearranged to PA. Further enzymatic reduction of PA leads to DPA (Fig 9, 4). Other metabolic steps, as reviewed in (13,14), include hydroxylation of the 7' and at National Research Council Canada on December 1, 2016 http://www.jbc.org/ Downloaded from 9'-methyl groups of the ABA ring as well as conjugation as glucose esters (ABAGE). Trans-ABA is a product of isomerization of natural ABA under UV light.…”

Section: Discussionmentioning

confidence: 99%

Phaseic Acid, an Endogenous and Reversible Inhibitor of Glutamate Receptors in Mouse Brain

Hou

Jiang

Zaharia

et al. 2016

Journal of Biological Chemistry

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Preparation of deuterated abscisic acid metabolites for use in mass spectrometry and feeding studies

Abstract: SummaryFour deuterium-labelled ABA catabolites of high isotopic purity were prepared for hormone profiling studies using HPLC-ESI-MS-MS. The catabolites, namely [7

Cited by 55 publications

References 33 publications

Abscisic acid metabolism and lipid accumulation of a cell suspension culture of Lesquerella fendleri

Abscisic acid metabolism and lipid accumulation of a cell suspension culture of Lesquerella fendleri

Gene Expression and Metabolite Profiling of Developing Highbush Blueberry Fruit Indicates Transcriptional Regulation of Flavonoid Metabolism and Activation of Abscisic Acid Metabolism

Phaseic Acid, an Endogenous and Reversible Inhibitor of Glutamate Receptors in Mouse Brain

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