Preparation of D-glucosamine oligosaccharides by the enzymatic hydrolysis of chitosan.

Abstract

In an earlier study,1} D-glucosamine oligosaccharides were prepared, using ion-exchange chromatography, from a partial hydrolyzate ofchitosan with HC1. The oligosaccharides from (GlcN)2* to (GlcN)5 were obtained by this

Purification and characterization of a novel chitinase from Burkholderia cepacia strain KH2 isolated from the bed log of Lentinus edodes, Shiitake mushroom.

“…Products of the hydrolysis were detected using highperformance liquid chromatography (HPLC) on TSKgel NH2-60 (4.6ϫ25 cm) following the method described by Izume and Ohtakara (1987) with (GlcNAc) 2-6 as the standard.…”

Purification and some properties of a novel chitosanase from Bacillus subtilis KH1.

“…Each chitooligosaccharide was dissolved in 10 mM Tris-HCl buffer, pH 7.0, to make a 10% (w/v) solution, and 0.2 U enzyme/g substrate was added; the mixture was incubated at 45°C for 1 h. The mixture was heated in a boiling water bath for 20 min to stop the reaction. Products of hydrolysis were detected by high-performance liquid chromatography (HPLC) on TSKgel NH2-60 (4.6ϫ25 cm) by the method of Izume and Ohtakara (1987), using GlcN-oligomers mixture containing GlcN-(GlcN) 6 from Seikagaku Kogyo Co., Ltd. as the standard.…”

Purification and Characterization of a Novel Type of Chitinase fromVibrio alginolyticusTK-22

“…4 ) Conventional hydrolytic procedures in an acidic medium are still inadequate due to the insufficient yields of chitooligosaccharides (5-3% yield of each DP 5-7 oligomer, in the case of hydrolysis by hydrogen fluoride),5) because a partial hydrolytic N-deacylation reaction is inevitable. In the case' of production of chitosan oligo saccharides, enzymatic hydrolysis of chitosan by bacterial chitosanase was recently proposed, 6,7) which produced di-to penta-saccharides in a high yield (above 60%). However no chitinase useful for the production of various chitooligosaccharides has yet been reported.…”