Preparation of cyanobacterial peptide toxin as a biopesticide against cotton pests

Sathiyamoorthy, P.; Shanmugasundaram, S.

doi:10.1007/s002530050852

Cited by 26 publications

(12 citation statements)

References 3 publications

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“…Some antifungal molecules derived from cyanobacteria have been patented for agricultural use, but research on this topic has not been continued (14,21). The insecticidal and nematocidal activity of cyanobacteria is still largely unexplored and only a few studies of this subject have been published (15,28,32). This is true also for their phytotoxic and herbicidal properties (8,13,18); one herbicidal compound, cyanobacterin, from Scytonema hofmanni, has been patented (11) but not commercially developed.…”

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confidence: 99%

Evaluation ofNostocStrain ATCC 53789 as a Potential Source of Natural Pesticides

Biondi

Piccardi

Margheri

et al. 2004

Appl Environ Microbiol

102

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The cyanobacterium Nostoc strain ATCC 53789, a known cryptophycin producer, was tested for its potential as a source of natural pesticides. The antibacterial, antifungal, insecticidal, nematocidal, and cytotoxic activities of methanolic extracts of the cyanobacterium were evaluated. Among the target organisms, nine fungi (Armillaria sp., Fusarium oxysporum f. sp. melonis, Penicillium expansum, Phytophthora cambivora, P. cinnamomi, Rhizoctonia solani, Rosellinia, sp., Sclerotinia sclerotiorum, and Verticillium albo-atrum) were growth inhibited and one insect (Helicoverpa armigera) was killed by the extract, as well as the two model organisms for nematocidal (Caenorhabditis elegans) and cytotoxic (Artemia salina) activity. No antibacterial activity was detected. The antifungal activity against S. sclerotiorum was further studied with both extracts and biomass of the cyanobacterium in a system involving tomato as a host plant. Finally, the herbicidal activity of Nostoc strain ATCC 53789 was evaluated against a grass mixture. To fully exploit the potential of this cyanobacterium in agriculture as a source of pesticides, suitable application methods to overcome its toxicity toward plants and nontarget organisms must be developed.

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confidence: 99%

Evaluation ofNostocStrain ATCC 53789 as a Potential Source of Natural Pesticides

Biondi

Piccardi

Margheri

et al. 2004

Appl Environ Microbiol

102

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“…Another insecticidal compound that showed toxicity against Helicoverpa armigera (Lepidoptera) was a glycine-rich peptide of Scytonema sp. (Sathiyamoorthy and Shanmugasundaram, 1996). Unsaturated fatty acids of Oscillatoria agardhii were found to be the cause of mortality in larvae of the mosquito Aedes albopictus (Harada et al, 2000), and more recently a known cryptophycin-producing strain of Nostoc sp.…”

Section: Discussionmentioning

confidence: 99%

Insecticidal compounds of the biofilm‐forming cyanobacterium Fischerella sp. (ATCC 43239)

Becher

Jüttner

2005

Environmental Toxicology

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Cyanobacterial biofilms are grazed by many different benthic invertebrates. In particular, larvae of insects are often present in great numbers and exert strong grazing pressure on cyanobacteria. Along with morphological adaptations, allelochemicals may have been developed as defense mechanisms against insect larvae. To investigate the chemical defense of biofilm-forming cyanobacteria, larvae of Chironomus sp., a widely distributed genus in this habitat, were used. Ten artificial biofilms of axenic and nonaxenic cyanobacteria were screened for insecticidal activity against Chironomus sp. Fischerella 43239 was the cyanobacterium that exhibited the highest acute toxicity. A bioassay-guided isolation procedure was used to study the compounds responsible for toxicity in more detail. A toxic fraction was obtained when the 60% methanolic extract of Fischerella 43239 was separated on a C18 HPLC column. Electrospray mass spectrometry indicated the presence of several compounds in this fraction. The successful separation into individual compounds was achieved by HPLC on a cyanopropyl column. The heavily clustered quasimolecular ions observed on an electrospray mass spectrometer and the absorption spectra of the separated compounds were indicative of indole derivatives. The existence in the benthic cyanobacterium Fischerella 43239 of strong insecticidal metabolites that serve as chemical protection agents against insect larvae is supported by the data.

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“…Microorganisms have been recognised as a major source of bioactive metabolites with antibacterial (Volk and Furkert 2006), antifungal (Bottone and Peluso 2003;Lavermicocca et al 2000), antiviral (Deng and Cliver 1995), anticancer (Williams 2009), insecticidal (Sathiyamoorthy and Shanmugasundaram 1996), algicidal (Blom et al 2006) and immunosuppressive properties (Burja et al 2001). Most of these compounds have been obtained from terrestrial actinomycetes and filamentous fungi (Zjawiony 2004;Fenical and Jensen 2006;Newman and Cragg 2007) but more recently from aquatic bacterial groups with a more diverse physiology like cyanobacteria (see reviews, Burja et al 2001;Dahms et al 2006;Abed et al 2009).…”

Section: Introductionmentioning

confidence: 99%

Cyanobacterial mats from hot springs produce antimicrobial compounds and quorum-sensing inhibitors under natural conditions

et al. 2010

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Polar (water) and non-polar (ethyl acetate) extracts from the cyanobacterial layer (top 1-3 mm) of four hot spring microbial mats in the Sultanate of Oman were tested for their antibacterial, antidiatom and quorumsensing inhibitory activities under natural conditions. The chemical composition of the active extracts was analysed using gas chromatography-mass spectrometry (GC-MS). Cyanobacteria within these mats were identified by direct microscopy while the total bacterial community composition was compared using automated ribosomal intergenic spacer analysis (ARISA). Only the extracts from Bowshar and Nakhl mats showed antibacterial properties against Bacillus sp., Micrococcus luteus, Shigella sonnei, Salmonella enterica and Klebsiella pneumoniae. All tested extracts inhibited the growth of the benthic diatom Amphora coffeaeformis. Extracts from Bowshar, Rustaq and Nakhl inhibited quorum-sensing of the reporter strains Chromobacterium violaceum CV017 and Agrobacterium tumefaciens NTL4.The highest bioactivity was recorded for ethyl acetate extracts from Nakhl mats, which had the lowest number of operational taxonomic units (OTUs). Using GC-MS, 74 chemical compounds were obtained, however with different distribution among the four mat extracts (similarity<43%). Various cyanobacteria, belonging mainly to Chroococcus, Phormidium, Leptolyngbya, Spirulina and Lyngbya were detected in the different mats, and each mat had its unique bacterial community, as confirmed by ARISA profiles. We conclude that antimicrobial and quorum-sensing inhibitory compounds can be produced by hot spring mat microorganisms under natural conditions and the differences in these compounds could be attributed to the differences in the mats' bacterial composition as well as the physical-chemical conditions of the springs.

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Preparation of cyanobacterial peptide toxin as a biopesticide against cotton pests

Cited by 26 publications

References 3 publications

Evaluation ofNostocStrain ATCC 53789 as a Potential Source of Natural Pesticides

Evaluation ofNostocStrain ATCC 53789 as a Potential Source of Natural Pesticides

Insecticidal compounds of the biofilm‐forming cyanobacterium Fischerella sp. (ATCC 43239)

Cyanobacterial mats from hot springs produce antimicrobial compounds and quorum-sensing inhibitors under natural conditions

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