Preparation of curcumin-loaded PCL-PEG-PCL triblock copolymeric nanoparticles by a microchannel technology

Guo, Fangyuan; Guo, Dingjia; Zhang, Wei; Yan, Qinying; Yang, Yan; Hong, Weiyong; Yang, Gensheng

doi:10.1016/j.ejps.2017.01.001

Cited by 41 publications

(41 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As shown in Fig. 10a, c, and d, an evidently weaker fluorescence intensity was observed for Cur-DMSO compared to Cur-NPs and Cur-P-NPs, indicating enhanced cellular uptake efficiencies because of the NPs [23]. Moreover, a stronger fluorescence intensity of Cur-P-NPs (Fig.…”

Section: Cellular Uptakementioning

confidence: 82%

“…The mixture was further stirred at 25 °C for 96 h until the H-NMR and FT-IR spectra of MePEG-Peptide-Tri-CL were recorded on a Bruker AVANCE III spectrometer and Nicolet 6700 spectrometer, respectively. The number average molecular weight (Mn), weight-average molecular weight (Mw), and polydispersity index (PDI) of the polymers in each reaction equation were analyzed by gel permeation chromatography (GPC) using an LC-20AT instrument with an RID-10A detector [23].…”

Section: Synthesis Of Mepeg-peptide-tri-clmentioning

confidence: 99%

See 1 more Smart Citation

Matrix metalloprotein-triggered, cell penetrating peptide-modified star-shaped nanoparticles for tumor targeting and cancer therapy

Guo

Zhou

et al. 2020

J Nanobiotechnol

Self Cite

View full text Add to dashboard Cite

Background: Specific targeting ability and good cell penetration are two critical requirements of tumor-targeted delivery systems. In the present work, we developed a novel matrix metalloprotein-triggered, cell-penetrating, peptide-modified, star-shaped nanoparticle (NP) based on a functionalized copolymer (MePEG-Peptide-Tri-CL), with the peptide composed of GPLGIAG (matrix metalloprotein-triggered peptide for targeted delivery) and r9 (cell-penetrating peptide for penetration improvement) to enhance its biological specificity and therapeutic effect. Results: Based on the in vitro release study, a sustained release profile was achieved for curcumin (Cur) release from the Cur-P-NPs at pH 7.4. Furthermore, the release rate of Cur was accelerated in the enzymatic reaction. MTT assay results indicated that the biocompatibility of polymer NPs (P-NPs) was inversely related to the NP concentration, while the efficiency toward tumor cell inhibition was positively related to the Cur-P-NP concentration. In addition, Cur-P-NPs showed higher fluorescence intensity than Cur-NPs in tumor cells, indicating improved penetration of tumor cells. An in vivo biodistribution study further demonstrated that Cur-P-NPs exhibited stronger targeting to A549 xenografts than to normal tissue. Furthermore, the strongest tumor growth inhibition (76.95%) was observed in Cur-P-NP-treated A549 tumor xenograft nude mice, with slight pulmonary toxicity. Conclusion: All results demonstrated that Cur-P-NP is a promising drug delivery system that possesses specific enzyme responsiveness for use in anti-tumor therapy.

show abstract

Section: Cellular Uptakementioning

confidence: 82%

Section: Synthesis Of Mepeg-peptide-tri-clmentioning

confidence: 99%

Matrix metalloprotein-triggered, cell penetrating peptide-modified star-shaped nanoparticles for tumor targeting and cancer therapy

Guo

Zhou

et al. 2020

J Nanobiotechnol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Polyethylene glycol (PEG) is a biocompatible and hydrolytically degradable polymer, therefore, PEG derivatives have been widely used in biomaterials and drug delivery. For example, PEC is one of ester-containing PEG used in tissue engineering, [35] nanotechnology [36,37] and protein/ drug controlled delivery [38,39] since the degradation rates can be controlled by the molecular structure of esters and the degree of branching of the PEGs. [40] Traditional acrylate-derivatized PEG (PEGDA) is a polyether-based hydrogel with endgroup acrylate esters, for that reason, its degradation is due to dydrolysis of the end group acrylate esters and oxidation of the ether backbone.…”

Section: Discussionmentioning

confidence: 99%

Synthesis, characterization, and cytotoxicity of PCL–PEG–PCL diacrylate and agarose interpenetrating network hydrogels for cartilage tissue engineering

Lin

Chang

et al. 2020

J of Applied Polymer Sci

View full text Add to dashboard Cite

Hydrogels are suitable biomaterials for cartilage tissue engineering due to the excellent ability to retain water to provide suitable environment for the tissue, however, the insufficient mechanical properties often prevent their wider applications. The objective of this study was to fabricate biocompatible hydrogels with good mechanical performance, high‐water content, and porous microstructure for cartilage regeneration. Photocrosslinked hydrogels are one of the most widely used systems in tissue engineering due to the superior mechanical properties. In this study, block copolymer, poly(ε ‐caprolactone)‐poly(ethylene)‐poly(ε‐caprolactone) diacrylate (PCL–PEG–PCL; PEC), was prepared by ring‐opening polymerization, and PEC hydrogels were made through free radical crosslinking mechanism. Agarose network is chosen as another component of the hydrogels, because of the high‐swelling behavior and cartilage‐like microstructure, which is helpful for chondrocytes growth. Interpenetrating networks (IPN) were fabricated by diffusing PEC into agarose network followed by photo‐crosslinking process. It was noted that incorporating PEC into the agarose network increased the elastic modulus and the compressive failure properties of individual component networks. In addition, high‐swelling ratio and uniform porosity microstructures were found in the IPN hydrogels. IPN and PEC showed low cytotoxicity and good biocompatibility in elution test method. The results suggest promising characteristics of IPN hydrogels as a potential biomaterial for cartilage tissue engineering.

show abstract

“…DCM solution was collected and added drop-wise to cold methanol (1:15, v/v). PCL-NH 2 was purified by precipitation, and obtained after filtration and vacuum drying at 40 °C for 24 h. The number average molecular weight (Mn), the weight-average molecular weight (Mw), and polydispersity index (PDI) of PCL-NH 2 were measured by Gel Permeation Chromatography (GPC) analysis [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

“…Thereafter, mPEG-NHS (49.9 mg, 0.0178 mmol) was mixed into the reaction solution, and transesterification was allowed to occur at room temperature for 24 h. The mPEG-Peptide solution was purified by dialysis (Mw: 3500) for 72 h, and lyophilized to the powder form. The Mn, Mw, and PDI were measured by GPC [ 33 ].…”

Section: Methodsmentioning

confidence: 99%

PEGylated self-assembled enzyme-responsive nanoparticles for effective targeted therapy against lung tumors

Guo

et al. 2018

J Nanobiotechnol

Self Cite

View full text Add to dashboard Cite

BackgroundMatrix-metalloproteinases, which are overexpressed in many types of cancer, can be applied to improve the bioavailability of chemotherapeutic drugs and guide therapeutic targeting. Thus, we aimed to develop enzyme-responsive nanoparticles based on a functionalized copolymer (mPEG-Peptide-PCL), which was sensitive to matrix metalloproteinase, as smart drug vesicles for enhanced biological specificity and reduced side effects.ResultsThe rate of in vitro curcumin (Cur) release from Cur-P-NPs was not markedly accelerated in weakly acidic tumor microenvironment, indicating a stable intracellular concentration and a consistent therapeutic effect. Meanwhile, P-NPs and Cur-P-NPs displayed prominent biocompatibility, biostability, and inhibition efficiency in tumor cells. In addition, Cur-P-NPs showed higher fluorescence intensity than Cur-NPs in tumor cells, implying enhanced cell permeability and targeting ability. Moreover, the internalization and intracellular transport of Cur-P-NPs were mainly via macropinocytosis. Studies of pharmacodynamics and cellular uptake in vitro and biodistribution in vivo demonstrated that Cur-P-NPs had stronger target efficiency and therapeutic effect than Cur-DMSO and Cur-NPs in tumor tissue.ConclusionResults indicate that Cur-P-NPs can be employed for active targeted drug delivery in cancer treatment and other biomedical applications.Electronic supplementary materialThe online version of this article (10.1186/s12951-018-0384-8) contains supplementary material, which is available to authorized users.

show abstract

Preparation of curcumin-loaded PCL-PEG-PCL triblock copolymeric nanoparticles by a microchannel technology

Cited by 41 publications

References 25 publications

Matrix metalloprotein-triggered, cell penetrating peptide-modified star-shaped nanoparticles for tumor targeting and cancer therapy

Matrix metalloprotein-triggered, cell penetrating peptide-modified star-shaped nanoparticles for tumor targeting and cancer therapy

Synthesis, characterization, and cytotoxicity of PCL–PEG–PCL diacrylate and agarose interpenetrating network hydrogels for cartilage tissue engineering

PEGylated self-assembled enzyme-responsive nanoparticles for effective targeted therapy against lung tumors

Contact Info

Product

Resources

About