Preparation of CS/PVA Nanofibrous Membrane with Tunable Mechanical Properties for Tympanic Member Repair

Huang, Mengjia; Li, Jie; Chen, Jia; Zhou, Mi; He, Jian‐Guo

doi:10.1007/s13233-018-6127-8

Cited by 10 publications

(6 citation statements)

References 32 publications

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“…The eggshell membrane was not flat, existed a certain curvature. Thus, the stress of the membrane was not uniform during the tensile testing and a step fracturing behavior was observed) and a piece of paper (7.5 ± 0.4 MPa, 7.8 ± 2.3%; which was also used as the control group in earlier reports), 39 the mechanical properties of the PCL/gelatin membrane were higher, and the adjustable mechanical properties will be better suitable to the repairing of TM perforation.…”

Section: Resultsmentioning

confidence: 95%

“…The tensile properties of PCL/gelatin membranes were evaluated using a WDW‐1 Universal Testing Machine (Songdun Machine Equipment Co., Ltd., China) at the wet state. Briefly, testing specimens were cut into rectangular membrane with a length of 50 mm and width of 10 mm and were tested at a strain rate of 0.5 mm/min at ambient conditions according to previous research 31,38,39 . The testing standard and processes refer to the International Standard (ISO 527‐3, Test conditions for films and sheets).…”

Section: Methodsmentioning

confidence: 99%

“…Briefly, testing specimens were cut into rectangular membrane with a length of 50 mm and width of 10 mm and were tested at a strain rate of 0.5 mm/min at ambient conditions according to previous research. 31,38,39 The testing standard and processes refer to the International Standard (ISO 527-3, Test conditions for films and sheets). The PCL/gelatin membranes with different proportions were measured.…”

Section: Mechanical Propertiesmentioning

confidence: 99%

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3D printing‐assistedcombinatorial approach for designing mechanically‐tunable and vascular supportive nanofibrous membranes to repair perforated eardrum

Liu

Huo

Zhang

et al. 2020

J of Applied Polymer Sci

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Perforation of eardrum or tympanic membrane (TM) is a common clinical condition, which occurs due to infection or injury of the eardrum, and could results in varying degrees of conductive hearing loss among all ages. In this study, the authors report the combinatorial approach of designing mechanically-tunable and vascular supportive nanofibrous membranes by 3D printing-assisted electrospinning (e-spin) using polycaprolactone (PCL) and gelatin with different mass ratios suitable to repair a perforated eardrum. The physicochemical, mechanical, and biological properties of the membranes were characterized. The results show that the membrane has nanofibrous morphology with fibers are of varying size (400-600 nm in diameter) depending on processing conditions. The wettability and mechanical properties of the membrane can be tuned by regulating the gelatin content. Moreover, a biomimetic repair strategy inspired by chicken eggshell membrane, often used in wound dressings, was also presented for study and results show that the suture retention strength of the fabricated membrane can meet clinical translational requirements to promote TM healing. The vascular cell responsiveness of PCL/gelatin nanofibrous membrane was evaluated using human umbilical vein endothelial cells (HUVECs) and the results showed satisfactory biocompatibility, vascular cell responsiveness, and cell proliferation. The findings of this study demonstrate that the combinatorically engineered PCL/gelatin nanofibrous membrane has great potential for repairing perforated eardrum.

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Section: Resultsmentioning

confidence: 95%

Section: Methodsmentioning

confidence: 99%

Section: Mechanical Propertiesmentioning

confidence: 99%

See 1 more Smart Citation

3D printing‐assistedcombinatorial approach for designing mechanically‐tunable and vascular supportive nanofibrous membranes to repair perforated eardrum

Liu

Huo

Zhang

et al. 2020

J of Applied Polymer Sci

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“…Both studies demonstrated that the cross-linking agent enhanced the mechanical and water resistance kinetic of nanofibers. The resulting scaffolds showed an improved survival rate of different cells and are biocompatible for tissue engineering of TMP [166,167]. The PEOT/PBT nanofibers hold superior structural and mechanical properties.…”

Section: Fibrous Scaffoldsmentioning

confidence: 96%

Necessities, opportunities, and challenges for tympanic membrane perforation scaffolding-based bioengineering

Hussain

Pei

2021

Biomed. Mater.

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Tympanic membrane (TM) perforation is a global clinical dilemma. It occurs as a consequence of object penetration, blast trauma, barotrauma, and middle ear diseases. TM perforation may lead to otitis media, retraction pockets, cholesteatoma, and conductive deafness. Molecular therapies may not be suitable to treat perforation because there is no underlying tissue matrix to support epithelium bridging. Chronic perforations are usually reconstructed with autologous grafts via surgical myringoplasty. Surgical treatment is uncomfortable for the patients. The grafting materials are not perfect because they produce an opaque membrane, fail in up to 20% of cases, and are suboptimal to restore acoustic function. Millions of patients from developing parts of the world have not got access to surgical grafting due to operational complexities, lack of surgical resources, and high cost. These shortcomings emphasize bioengineering to improve placement options, healing rate, hearing outcomes, and minimize surgical procedures. This review highlights cellular, structural, pathophysiological, and perforation specific determinants that affect healing, acoustic and surgical outcomes; and integrates necessities relevant to bioengineered scaffolds. This study further summarizes scaffolding components, progress in scaffolding strategies and design, and engenders limitations and challenges for optimal bioengineering of chronic perforation.

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“…Tympanic membrane perforations have also been treated with electrospun scaffolds. Like corneal applications, TM electrospun scaffolds typically combine natural and synthetic polymers to achieve the necessary mechanics for surgical handling, cell attachment, and proliferation. , Immich et al utilized a poly(lactic acid) (PLLA)/PLGA copolymer electrospinning technique for myringoplasties in rats . These membranes were seeded with fibroblast and keratinocyte cocultures, producing 100% recovery of TM perforations in all experimental groups.…”

Section: Fabrication Strategiesmentioning

confidence: 99%

Fabrication Strategies for Engineered Thin Membranous Tissues

McLoughlin

McKenna

Fisher

2023

ACS Appl. Bio Mater.

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Thin membranous tissues (TMTs) are anatomical structures consisting of multiple stratified cell layers, each less than 100 μm in thickness. While these tissues are small in scale, they play critical roles in normal tissue function and healing. Examples of TMTs include the tympanic membrane, cornea, periosteum, and epidermis. Damage to these structures can be caused by trauma or congenital disabilities, resulting in hearing loss, blindness, dysfunctional bone development, and impaired wound repair, respectively. While autologous and allogeneic tissue sources for these membranes exist, they are significantly limited by availability and patient complications. Tissue engineering has therefore become a popular strategy for TMT replacement. However, due to their complex microscale architecture, TMTs are often difficult to replicate in a biomimetic manner. The critical challenge in TMT fabrication is balancing fine resolution with the ability to mimic complex target tissue anatomy. This Review reports existing TMT fabrication strategies, their resolution and material capabilities, cell and tissue response, and the advantages and disadvantages of each technique.

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Preparation of CS/PVA Nanofibrous Membrane with Tunable Mechanical Properties for Tympanic Member Repair

Cited by 10 publications

References 32 publications

3D printing‐assistedcombinatorial approach for designing mechanically‐tunable and vascular supportive nanofibrous membranes to repair perforated eardrum

3D printing‐assistedcombinatorial approach for designing mechanically‐tunable and vascular supportive nanofibrous membranes to repair perforated eardrum

Necessities, opportunities, and challenges for tympanic membrane perforation scaffolding-based bioengineering

Fabrication Strategies for Engineered Thin Membranous Tissues

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