Preparation of crystalline Pseudomonas cytochrome c-551 and its general properties

Abstract

A simple method has already been devised for extensive purification of four respiratory components from P8eudomona8 aerugino8a in a true water-soluble state without the aid of detergents (Horio, 1958a). These components were called P8eudomona8 (P)-cytochrome-554, P-cytochrome-551, P-blue protein and P-cytochrome oxidase (Horio, 1958b; Horio, Higashi, Matsubara et al. 1958). The same microorganism also contains another cytochrome, P-cytochrome-560, which is similar to the so-called cytochrome b, and which has not yet been solubilized without the aid of sodium cholate (Horio, 1958a). From studies on purified respiratory components, cellular fragments and whole cells, Horio (1958b) and Yamanaka (1959) have concluded that in the electron-transferring system of P. aerugino8a P-cytochrome-551 and

Amino acid sequence of cytochrome c-552 from a thermophilic hydrogen-oxidizing bacterium, Hydrogenobacter thermophilus

“…The facts that the methionine residue at position 59 is surrounded by a cluster of proline residues and that lysine is the carboxyl-terminal residue are also typical features of Pseudomonas cytochromes c-551. Cytochrome c-552 has, however, a preponderance of basic amino acid residues (15 residues) to acidic ones (7 residues), as reflected by its basic isoelectric point of above 10 (data not shown), while cytochrome c-551 is an acidic protein (pI, 4.70 [9] …”

Nuclear‐Magnetic‐Resonance Studies of Pseudomonas aeruginosa Cytochrome c‐551

“…Ferrocytochrome c-551 concentrations were determined by inspection of the 551-nm absorption using a Pye Unicam SP8000 ultraviolet spectrophotometer. The 551 -nm absorption coefficient was taken to be 28.3 x lo3 M-' cm-' [17]. The NMR spectra were obtained using a Bruker 270-MHz spectrometer operating in the Fourier transform mode, with an Oxford Instruments Co. magnet.…”

Thiobacillus novellus cytochrome oxidase can separate some eucaryotic cytochromes c

“…Highly purified cytochrome c (550, Thiobacillus novellus) [4], cytochrome c (551, Pseudomonas aeruginosa) [5], cytochrome c (555, Chlorobium thiosulfatophilum) [6], cytochrome c (552, Nitrosomonas europaea) [21] cow cytochrome c [7], tuna cytochrome c [8] and man cytochrome c [9] were obtained by the methods previously established. Cytochrome c (Saccharomyces oviformis) (iso-1 -) [lo] and cytochrome c2 (Rhodospirillum rubrum) [ 111 were kindly supplied by Sankyo Co. Ltd (Tokyo, Japan) and Dr T. Horio (Institute for Protein Research, Osaka University, Osaka, Japan), respectively.…”