Preparation of corn (<i>Zea mays</i>) peptides and their protective effect against alcohol‐induced acute hepatic injury in NH mice

Hong-mei, LI; Hu, Xin; Xu, Lina; Zhang, Xuezhong

doi:10.1042/ba20060183

Cited by 16 publications

(10 citation statements)

References 19 publications

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“…The F4 from CH-P (CH-P4), F1 from CH-F (CH-F1), and F3 from CH-B (CH-B3) with promising antioxidant activities were identified through RP-HPLC and MALDI-TOF/TOF MS for peptide compositions ( Tables S1–S3, Supplementary Document ). Numerous peptide sequences were observed for each peptide fraction due to the complex protein composition in CGM, and there were high levels of Glu, Pro, Ala, Leu, Phe, and Tyr in all peptides which agreed with the studies of Li et al [ 41 ] and Hu et al [ 42 ]. Antioxidant properties of protein hydrolysates were related to their composition and structure.…”

Section: Resultssupporting

confidence: 90%

Production and Characterization of Antioxidative Hydrolysates and Peptides from Corn Gluten Meal Using Papain, Ficin, and Bromelain

Chen

2020

Molecules

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There has been a growing interest in developing natural antioxidants with high efficiency and low cost. Bioactive protein hydrolysates could be a potential source of natural and safer antioxidants. The objectives of this study were to hydrolyze corn gluten meal using three plant-derived proteases, namely papain, ficin, and bromelain, to produce antioxidative hydrolysates and peptides and to characterize the antioxidant performances using both chemical assays and a ground meat model. The optimum hydrolysis time for papain was 3 h, and for ficin and bromelain was 4 h. The hydrolysates were further separated by sequential ultrafiltration to 5 hydrolysate fractions named F1 to F5 from low molecular weight (MW) (<1 kDa) to high MW range (>10 kDa), which were further characterized for TPC, free radical scavenging capacity against DPPH and ABTS, and metal chelating activity. The fraction F4 produced by papain (CH-P4), F1 produced by ficin (CH-F1), and F3 produced by bromelain (CH-B3) showed the strongest antioxidant activity and yield, respectively. These three fractions were incorporated into ground pork to determine their inhibition effects on lipid oxidation during a 16-day storage period. The inhibition effect was enhanced with the addition of higher amount of hydrolysate (e.g., 1000 vs. 500 mg/kg). The CH-P4 reduced lipid oxidation in ground meat by as much as 30.45%, and CH-B3 reduced oxidation by 27.2% at the same level, but the inhibition was only 13.83% with 1000 mg/kg of CH-F1. The study demonstrated that CGM protein hydrolysates and peptides could be used as naturally derived antioxidant in retarding lipid oxidation and improving product storage stability.

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Section: Resultssupporting

confidence: 90%

Production and Characterization of Antioxidative Hydrolysates and Peptides from Corn Gluten Meal Using Papain, Ficin, and Bromelain

Chen

2020

Molecules

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“…Alcohol-soluble zein can be separated from corn by four methods including dry milling, wet milling, alkaline processing, and the dry grind processing with ethanol (Shukla & Cheryan, 2001). The most abundant amino acids in CGM are Glu, Leu, Pro, Ala, Phe, and Asp (Li, Guo, Hu, Xu, & Zhang, 2007). However, its properties of low water Review of corn proteins-derived bioactive peptides .…”

Section: Introductionmentioning

confidence: 99%

A Comprehensive Review of Corn Protein‐derived Bioactive Peptides: Production, Characterization, Bioactivities, and Transport Pathways

Zhu

Hou

2018

Comp Rev Food Sci Food Safe

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Bioactive peptides are specific peptide fragments that positively exert various functional and biological activities and ultimately influence health. Corn protein are potential precursor proteins for bioactive peptides. This review encompasses the studies reported to date on the production, isolation, purification, and characterization technologies of bioactive corn peptides (CPs), with particular attention being devoted to these peptides’ different health effects, including antioxidant, antihypertensive, hepatoprotective, alcohol‐metabolism‐facilitating, anti‐inflammatory, anticancer, antimicrobial, and dipeptidyl peptidase IV (DPP‐IV) inhibitory activities. The review also describes studies examining the potential mechanisms believed to be involved in these bioactivities, and the possible absorption and transport pathways of CPs are summarized.

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“…Previous studies have demonstrated that CPs possess various bioactive functions, such as anti-hypertension [ 23 ], antioxidant [ 6 , 8 – 14 ], improvements in lipid profile [ 9 ], facilitation of alcohol metabolism [ 16 , 24 , 25 ], prevention of hepatocellular carcinoma [ 26 ], and protection against alcohol- [ 9 , 10 ] and other hepatotoxic substances-induced liver injury [ 11 , 12 ], but limited data are available for the effect of CPs in humans. The present randomized trial showed for the first time that CPs supplementation (4 g/d) for 9 weeks had beneficial effects on alcoholic liver injury in men with chronic alcohol consumption and fatty liver, whereas the same dose of WP and placebo did not have these effects.…”

Section: Discussionmentioning

confidence: 99%

“…An increase in the NADH/NAD + ratio favors the deposits of TG in the liver and results in steatosis [ 27 , 28 ]. Previously, Li et al observed a reduction in the liver TG levels after CPs supplementation in a mouse model of acute alcoholic liver injury [ 10 ]. Zhang et al observed a reduction in serum TC, LDL-C and HDL-C levels after CPs supplementation in a rat model of early alcoholic liver injury [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

“…However, CGM is mainly used as a feedstuff or discarded because of its low water solubility and severe amino acid imbalance even though it contains approximately 60% (w/w) protein [ 6 , 7 ]. In recent years, corn peptides (CPs), a novel food prepared from CGM by enzymatic hydrolysis, have attracted considerable interest due to various bioactive properties, including antioxidant activity [ 6 , 8 – 14 ], improvements in lipid profiles [ 9 ] and the ability to accelerate alcohol metabolism [ 15 , 16 ] and protect against alcohol-induced liver injury [ 9 , 10 ]. The research and development of CPs might effectively increase the value of CGM in the marketplace.…”

Section: Introductionmentioning

confidence: 99%

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Protective effect of corn peptides against alcoholic liver injury in men with chronic alcohol consumption: a randomized double-blind placebo-controlled study

Pan

Zhang

et al. 2014

Lipids Health Dis

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BackgroundCorn peptides (CPs) are a novel food prepared from corn gluten meal, which is a main by-product of the corn starch industry. Recently, significant beneficial effects of CPs on early alcoholic liver injury in rats and on acute alcoholic injury in mice were observed. To our knowledge, the present study is the first report showing that CPs supplementation has beneficial effects on lipid profile, oxidative stress and alcoholic liver injury in men with chronic alcohol consumption.MethodsA 9-week, randomized, double-blind, placebo-controlled study was conducted between September 2011 and August 2012 to assess the hepatoprotective effect of CPs. A total of 161 men were randomized to receive CPs (n = 53), whey protein (n = 54), or corn starch placebo (n = 54) at the same dose of 2 g twice daily. 146 participants completed the study. Serum lipid profile, serum markers of liver injury, oxidative stress and inflammation, and fatty liver based on the results of abdominal ultrasonography were assessed at the beginning and end of the intervention.ResultsCPs supplementation (4 g/d) for 9 weeks significantly lowered serum levels or activities of total cholesterol, triglyceride, alanine aminotransferase, aspartate aminotransferase, malondialdehyde and tumor necrosis factor-α, and significantly increased serum activities of superoxide dismutase and glutathione peroxidase, but the same dose of whey protein and corn starch (placebo) did not demonstrate these effects.ConclusionsOur results indicate that CPs may have protective effects on alcohol-induced liver damage via modulation of lipid metabolism and oxidative stress. CPs may potentially be used as a functional food for the management of alcoholic liver disease in subjects with chronic alcohol consumption.Electronic supplementary materialThe online version of this article (doi:10.1186/1476-511X-13-192) contains supplementary material, which is available to authorized users.

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Preparation of corn (Zea mays) peptides and their protective effect against alcohol‐induced acute hepatic injury in NH mice

Cited by 16 publications

References 19 publications

Production and Characterization of Antioxidative Hydrolysates and Peptides from Corn Gluten Meal Using Papain, Ficin, and Bromelain

Production and Characterization of Antioxidative Hydrolysates and Peptides from Corn Gluten Meal Using Papain, Ficin, and Bromelain

A Comprehensive Review of Corn Protein‐derived Bioactive Peptides: Production, Characterization, Bioactivities, and Transport Pathways

Protective effect of corn peptides against alcoholic liver injury in men with chronic alcohol consumption: a randomized double-blind placebo-controlled study

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