Preparation of chylomicron remnant-like particles for studying the effect of dietary lipids on vasoconstriction in porcine coronary arteries

Goulter, Andrew B.; Avella, Michael; Elliott, Jonathan; Botham, Kathleen M.

doi:10.1016/s0021-9150(99)80492-4

Cited by 2 publications

(7 citation statements)

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“…Analysis of the lipid composition of the particles before and after the incubation with plasma showed that the triacylglycerol\total cholesterol ratio was not changed by the procedure. In addition, we have found that CRLPs prepared in this way contain apoE and no other apolipoproteins, and are of a size consistent with that reported for chylomicron remnants in vivo [34].…”

Section: Preparation and Oxidation Of Crlpssupporting

confidence: 84%

“…57 nm [34], which is comparable with that found for chylomicron remnants from in vivo sources [7]. Thus, in terms of their size, density and lipid composition, the CRLPs resemble native chylomicron remnants, and in addition they contain human apoE.…”

Section: Discussionsupporting

confidence: 72%

“…containing apoB48) on cholesteryl ester synthesis in a mouse liver cell line [56], and the results were similar to those found here with trilinolein CRLPs and THP-1 cells, in that oxidation of remnants abolished the decrease observed with native particles. Furthermore, we have also demonstrated that oxidized rat chylomicron remnants inhibit vasorelaxation by interfering with NO production [10,57], and that equivalent effects are brought about in pig coronary arteries by oxidized CRLPs containing pig apoE [34,58]. Oxidized CRLPs, therefore, appear in many respects to behave in a similar way to chylomicron remnants containing apoB48.…”

Section: Discussionmentioning

confidence: 83%

“…Thus, in terms of their size, density and lipid composition, the CRLPs resemble native chylomicron remnants, and in addition they contain human apoE. Furthermore, similar particles containing apoE from the relevant species have been found to have effects which mimic those of physiological remnants in rat hepatocytes and in pig endothelial cells [27,34,37,38]. We conclude, therefore, that the CRLPs are suitable for the study of the influence of chylomicron remnants on lipid metabolism in THP-1 macrophages in the absence of other lipoproteins such as chylomicrons and VLDL, which may be present in remnants obtained from in vivo sources.…”

Section: Discussionmentioning

confidence: 97%

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Lipid synthesis in macrophages derived from the human cell line THP-1: modulation of the effects of native and oxidized chylomicron-remnant-like particles by oestrogen

Napolitano¹,

BATT²,

Avella³

et al. 2001

Clinical Science

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The effects of native and oxidized chylomicron remnants on the synthesis of cholesteryl ester and triacylglycerol in macrophages, and the way that this is influenced by exposure of the cells to oestrogen, was investigated using the human monocyte cell line THP-1 and chylomicron-remnant-like particles containing human apolipoprotein E (CRLPs). Synthesis of the lipids was measured by the incorporation of [(3)H]oleate into cholesteryl ester and triacylglycerol. CRLPs (5-40 microgram of cholesterol/ml) containing either trilinolein or triolein as the triacylglycerol component caused a dose-dependent decrease in cholesteryl ester formation, while triacylglycerol production was unchanged. After oxidation of the CRLPs, the level of thiobarbituric acid-reactive substances was increased by 6.3-fold and 2.2-fold in particles containing trilinolein and triolein respectively. Furthermore, CRLPs containing oxidized trilinolein lost their ability to down-regulate cholesterol esterification, while CRLPs containing oxidized triolein did not. Both types of oxidized CRLPs decreased triacylglycerol synthesis. Treatment of the macrophages with 17beta-oestradiol caused increases of approx. 94% and 34% in the synthesis of cholesteryl ester and triacylglycerol respectively in the absence of CRLPs. The differences between control and oestrogen-treated cells were abolished, however, when CRLPs (40 microgram of cholesterol/ml) were added to the incubations. In addition, in contrast with their lack of effect in control cells, CRLPs containing oxidized trilinolein decreased cholesterol esterification in oestrogen-treated cells by approx. 48%. These findings with CRLPs suggest that chylomicron remnants have significant effects on cholesteryl ester and triacylglycerol synthesis in macrophages, which may be modulated both by the oxidation state of the particles and by oestrogen.

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Section: Preparation and Oxidation Of Crlpssupporting

confidence: 84%

Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 97%

See 2 more Smart Citations

Lipid synthesis in macrophages derived from the human cell line THP-1: modulation of the effects of native and oxidized chylomicron-remnant-like particles by oestrogen

Napolitano¹,

BATT²,

Avella³

et al. 2001

Clinical Science

View full text Add to dashboard Cite

show abstract

“…In addition, we have found that CRLPs prepared in this way contain apoE and no other apolipoproteins, and are of a size consistent with that reported for chylomicron remnants in vivo [34]. The plasma was dialysed extensively against 0.9 % NaCl, then centrifuged in a fixed-angle rotor at 85 000 g for 16 h at 4 mC.…”

Section: Preparation and Oxidation Of Crlpssupporting

confidence: 67%

Lipid synthesis in macrophages derived from the human cell line THP-1: modulation of the effects of native and oxidized chylomicron-remnant-like particles by oestrogen

et al. 2001

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Preparation of chylomicron remnant-like particles for studying the effect of dietary lipids on vasoconstriction in porcine coronary arteries

Cited by 2 publications

References 0 publications

Lipid synthesis in macrophages derived from the human cell line THP-1: modulation of the effects of native and oxidized chylomicron-remnant-like particles by oestrogen

Lipid synthesis in macrophages derived from the human cell line THP-1: modulation of the effects of native and oxidized chylomicron-remnant-like particles by oestrogen

Lipid synthesis in macrophages derived from the human cell line THP-1: modulation of the effects of native and oxidized chylomicron-remnant-like particles by oestrogen

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