Preparation of chlorin e6–monoclonal antibody conjugate and its effect for photodynamic therapy

Ogura, Shun‐ichiro; Fujita, Yutaka; Kamachi, Toshiaki; Okura, Ichiro

doi:10.1002/jpp.351

Cited by 13 publications

(11 citation statements)

References 10 publications

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“…Zinc tetracarboxy-phthalocyanine (ZnTCPc) conjugated with antitumor antibody [33]. MM46 cells were used as tumor cells, and anti-MM46 MA (IgG) was used as the antitumor antibody.…”

Section: Implovement Of Photosensitizers By the Conjugation With Biommentioning

confidence: 99%

Improvement of Tumor Localization of Photosensitizers for Photodynamic Therapy and Its Application for Tumor Diagnosis

Ogura¹,

Hagiya²,

Tabata³

et al. 2012

CTMC

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Photodynamic therapy (PDT) and photodynamic diagnosis of cancer are widely used in clinical fields. These are performed using photosensitizers. Many metalloporphyrin-related compounds have been developed as photosensitizers for use in PDT, and these tumor localization ability have been improved in recent research. Moreover, the precursor of porphyrin 5-aminolevulinic acid is used in fluorescence diagnosis using its tumor localization ability. In this review, these applications of photosensitizers in cancer therapy and diagnosis are summarized.

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“…Zinc tetracarboxy-phthalocyanine (ZnTCPc) conjugated with antitumor antibody [33]. MM46 cells were used as tumor cells, and anti-MM46 MA (IgG) was used as the antitumor antibody.…”

Section: Implovement Of Photosensitizers By the Conjugation With Biommentioning

confidence: 99%

Improvement of Tumor Localization of Photosensitizers for Photodynamic Therapy and Its Application for Tumor Diagnosis

Ogura¹,

Hagiya²,

Tabata³

et al. 2012

CTMC

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“…Ce6 was conjugated with the anti-tumor monoclonal antibody (IgG) to increase its binding affinity for tumors [27,28]. Ce6 was activated by N-hydroxysulfosuccinimide and conjugated with IgG via peptide bonds (IgG-Ce6), and their binding affinities to tumor cells were investigated.…”

Section: Chlorin and Its Derivativesmentioning

confidence: 99%

Development of phthalocyanines for photodynamic therapy

Ogura

Tabata

Fukushima

et al. 2006

J. Porphyrins Phthalocyanines

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“…Confocal fluorescence imaging of cells (Figure 3b) uncovered that the Ce6 fluorescence inside cells incubated with PEDOT:PSS‐PEG‐Ce6 was much stronger than those treated with free Ce6, even though the fluorescence of Ce6 in the former formation was partially quenched due to its loading on PEDOT:PSS‐PEG (Supporting Information, Figure S4b). To quantitatively determine the cellular uptake of Ce6, we extracted the Ce6 molecules from cell lysate samples following the Ogura procedure 49. Ce6 molecules loaded on PEDOT:PSS‐PEG were completely detached after the strong base treatment as indicated by the ≈100% fluorescent recovery, likely owing to the deprotonation of carboxyl group of Ce6 molecules under the basic condition.…”

Section: Resultsmentioning

confidence: 99%

Near‐Infrared Absorbing Polymeric Nanoparticles as a Versatile Drug Carrier for Cancer Combination Therapy

Gong

Cheng

Xiang

et al. 2013

Adv Funct Materials

162

118

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Poly(3,4‐ethylenedioxythiophene):poly(4‐styrenesulfonate) (PEDOT:PSS) nanoparticles, after being coated with polyethylene glycol (PEG), are used as a drug carrier to load various types of aromatic therapeutic molecules, including chemotherapy drugs doxorubicin (DOX) and SN38, as well as a photodynamic agent chlorin e6 (Ce6), through π–π stacking and hydrophobic interaction. Interesting functionalities of PEDOT:PSS‐PEG as an unique versatile drug delivery platform are discovered. Firstly, for water‐insoluble drugs such as SN38, the loading on PEDOT:PSS‐PEG dramatically enhances its water solubility, while maintaining its cytotoxicity to cancer cells. Secondly, the delivery of Ce6 by PEDOT:PSS‐PEG is able to remarkably accelerate the cellular uptake of Ce6 molecules, and thus offers improved photodynamic therapeutic efficacy. Using DOX‐loaded PEDOT:PSS‐PEG as the model system, it is demonstrated that the photothermal effect of PEDOT:PSS‐PEG can be utilized to promote the delivery of this chemotherapeutic agent, achieving a combined photothermal‐ and chemotherapy with an obvious synergistic cancer killing effect. Moreover, it is also shown that multiple types of therapeutic agents could be simultaneously loaded on PEDOT:PSS‐PEG nanoparticles and delivered into cancer cells. This work highlights the great potential of NIR‐absorbing polymeric nanoparticles as multifunctional drug carriers for potential cancer combination therapy with high efficacy.

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Preparation of chlorin e6–monoclonal antibody conjugate and its effect for photodynamic therapy

Cited by 13 publications

References 10 publications

Improvement of Tumor Localization of Photosensitizers for Photodynamic Therapy and Its Application for Tumor Diagnosis

Improvement of Tumor Localization of Photosensitizers for Photodynamic Therapy and Its Application for Tumor Diagnosis

Development of phthalocyanines for photodynamic therapy

Near‐Infrared Absorbing Polymeric Nanoparticles as a Versatile Drug Carrier for Cancer Combination Therapy

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