Preparation of chitosan scaffolds with a hierarchical porous structure

Ko, Young-Gwang; Naoki, Katsuhiko; Tateishi, Tetsuya; Chen, Guoping

doi:10.1002/jbm.b.31586

Cited by 46 publications

(24 citation statements)

References 31 publications

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“…Moreover, the interaction of skin cells with chitosan-based materials in vitro and in vivo are not completely understood, which is the major obstacle for its introduction into bioengineering of human skin. For instance, some authors observed an inhibitory effect of chitosan on the growth of skin fi broblasts [18], while others reported its stimulating effect on proliferation of these cells [10,15,16,27]. Thus, the prospect of using chitosan for the development of artifi cial skin equivalents is an open question and requires additional comparative studies.…”

mentioning

confidence: 94%

Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering

et al. 2015

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We compared the structure and mechanical properties of scaffolds based on pure collagen, pure chitosan, and a mixture of these polymers. The role of the composition and structure of scaffolds in the maintenance of cell functions (proliferation, differentiation, and migration) was demonstrated in two experimental models: homogeneous tissue analogues (scaffold populated by fibroblasts) and complex skin equivalents (fibroblasts and keratinocytes). In contrast to collagen scaffolds, pure chitosan inhibited the growth of fibroblasts that did not form contacts with chitosan fibers, but formed specific cellular conglomerates, spheroids, and lose their ability to synthesize natural extracellular matrix. However, the use of chitosan as an additive stimulated proliferative activity of fibroblasts on collagen, which can be associated with improvement of mechanical properties of the collagen scaffolds. The effectiveness of chitosan as an additional cross-linking agent also manifested in its ability to improve significantly the resistance of collagen scaffolds to fibroblast contraction in comparison with glutaraldehyde treatment. Polymer scaffolds (without cells) accelerated complete healing of skin wounds in vivo irrespective of their composition healing, pure chitosan sponge being most effective. We concluded that the use of chitosan as the scaffold for skin equivalents populated with skin cells is impractical, whereas it can be an effective modifier of polymer scaffolds.

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mentioning

confidence: 94%

Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering

et al. 2015

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“…As such, optimal templates can be selected to address the dimensions and biocompatibility. Materials including poly(lactic-coglycolic acid) (PLGA) [474], polycaprolactone (PCL) [475], polyethylene glycol (PEG)-poly(2-hydroxyethyl methacrylate) (pHEMA) [476], chitosan [477], hydroxyapatite [478], and bioglasses [479] with low content of SiO 2 are designed and prepared through templating.…”

Section: Scaffolds and Membranesmentioning

confidence: 99%

“…In Soft templating through emulsion systems utilizing poly(Llactic acid)-grafted hydroxyapatite (g-HAp) nanoparticle templates stabilized in PLGA [485] and foamed surfactant solutions [486] have also produced scaffolds with porosity on the order of micrometers in diameter. Facile template removal is illustrated using ice crystals done by Ko et al [477] or PMMA microspheres [476] produced micrometer-sized, porous chitosan or PEG-pHEMA scaffolds, respectively.…”

Section: Scaffolds and Membranesmentioning

confidence: 99%

Template-based syntheses for shape controlled nanostructures

Pérez-Page

et al. 2016

Advances in Colloid and Interface Science

117

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A variety of nanostructured materials are produced through template-based synthesis methods, including zerodimensional, one-dimensional, and two-dimensional structures. These span different forms such as nanoparticles, nanowires, nanotubes, nanoflakes, and nanosheets. Many physical characteristics of these materials such as the shape and size can be finely controlled through template selection and as a result, their properties as well. Reviewed here are several examples of these nanomaterials, with emphasis specifically on the templates and synthesis routes used to produce the final nanostructures. In the first section, the templates have been discussed while in the second section, their corresponding synthesis methods have been briefly reviewed, and lastly in the third section, applications of the materials themselves are highlighted. Some examples of the templates frequently encountered are organic structure directing agents, surfactants, polymers, carbon frameworks, colloidal sol-gels, inorganic frameworks, and nanoporous membranes. Synthesis methods that adopt these templates include emulsion-based routes and template-filling approaches, such as self-assembly, electrodeposition, electroless deposition, vapor deposition, and other methods including layer-by-layer and lithography. Templatebased synthesized nanomaterials are frequently encountered in select fields such as solar energy, thermoelectric materials, catalysis, biomedical applications, and magnetowetting of surfaces.

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“…A lower freezing temperature resulted in smaller pores. [18] Mechanical Strength of Collagen-GAG and Collagen Sponges…”

Section: Collagen-gag and Collagen Sponges Prepared With Ice Particulmentioning

confidence: 99%

“…This method has been used to fabricate funnel-like chitosan sponges that had open surface pores and interconnected bulk pores to facilitate homogenous spatial cell distribution. [18] In this study, the ice particulate template method was used for the preparation of six types of funnel-like collagen and collagen-GAG sponges with controlled pore structures. Collagen is a natural polymer that is widely used as a biomaterial.…”

Section: Introductionmentioning

confidence: 99%

Preparation of Collagen‐Glycosaminoglycan Sponges with Open Surface Porous Structures Using Ice Particulate Template Method

Grice

Naoki

et al. 2010

Macromolecular Bioscience

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Funnel-like sponges of collagen incorporated with glycosaminoglycan (GAG) were prepared by freeze-drying using ice particulates as templates. The funnel-like collagen-GAG sponges showed similar porous structures to those of funnel-like collagen sponges. The funnel-like collagen-GAG and collagen sponges have one top surface layer and one bulk porous layer. The large, top surface pores were determined by ice particulates that were used as templates, and the inner bulk pores were determined by freezing temperature. The funnel-like pore structures facilitated homogenous cell distribution, improved cell viability, and resulted in homogenous tissue formation. Incorporation of GAG increased the mechanical property and cell viability of collagen sponges.

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Preparation of chitosan scaffolds with a hierarchical porous structure

Cited by 46 publications

References 31 publications

Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering

Chitosan as a Modifying Component of Artificial Scaffold for Human Skin Tissue Engineering

Template-based syntheses for shape controlled nanostructures

Preparation of Collagen‐Glycosaminoglycan Sponges with Open Surface Porous Structures Using Ice Particulate Template Method

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