Preparation of chitosan nanoparticles loaded with Balanites aegyptiaca extract for treatment of streptozotocin-induced diabetes in rats

Alahmer, Shimaa; El-Noss, Mostafa; Farid, Alyaa

doi:10.1016/j.ijbiomac.2024.130061

Cited by 1 publication

(2 citation statements)

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“…Diabetes induction was performed by the intravenous injection of STZ (65 mg/kg b.w.) after 6 h of fasting (water was provided ad libitum through the fasting hours) 19 . Rats were provided with sucrose dissolved in drinking water (10%) on the first day after STZ injection.…”

Section: Methodsmentioning

confidence: 99%

“…Pancreases were collected, from all experimental animals, fixed with buffered formalin (10%), dehydrated in an ascending grades of alcohol, cleared by xylene and embedded in paraffin blocks. By a microtome, pancreatic blocks were cut into 4 μm sections and stained with hematoxylin and eosin (H&E) stain 19 . For insulin immunohistochemical examination, pancreatic sections were deparrafinized, rehydrated and washed.…”

Section: Methodsmentioning

confidence: 99%

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Desert date seed extract-loaded chitosan nanoparticles ameliorate hyperglycemia and insulin deficiency through the reduction in oxidative stress and inflammation

Farid,

Ahmed,

Alaa

et al. 2024

Sci Rep

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Plants represents a huge source of bioactive materials that have been used since the old times in the treatment of many diseases. Balanites aegyptiaca, known as desert date, has been used in treatment of fever, diabetes and bacterial infection. Desert dates contains a hard seed that resembles 50–60% of the fruit. The seed extract contains many fatty acids, amino acids and other bioactive materials that gives the extract its antioxidant and anti-inflammatory properties. The study aimed to use Balanites seed extract-loaded chitosan nanoparticles (SeEx-C NPs) for the treatment of streptozotocin (STZ)-induced diabetes in male Sprague Dawley rats. Animals were divided into two main divisions (healthy and diabetic rats). Each division contained seven groups (5 rats/group): control untreated group I, SeEx treated group II and group III (10 and 20 mg/kg b.w., respectively), C NPs treated group IV and group V (10 and 20 mg/kg b.w., respectively) and SeEx-C NPs treated group VI and group VII (10 and 20 mg/kg b.w., respectively). The therapeutical effects of SeEx-C NPs were evaluated through biochemical and immunological assessments in rats’ pancreases. The results showed that SeEx-C NPs (10 and 20 mg/kg b.w.) reduced the oxidative stress and inflammation in rats’ pancreases allowing the islets neogenesis. The loading of SeEx on C NPs allowed the delivery of fatty acids (oleic, lauric and myristic acid), amino acids (lysine, leucine, phenylalanine and valine) and minerals to pancreatic beta-cells in a sustainable manner. SeEx-C NPs administration successfully increased insulin secretion, allowed pancreatic islets neogenesis and reduced oxidative stress and inflammation.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%