Preparation of chitosan nanoparticles by TPP ionic gelation combined with spray drying, and the antibacterial activity of chitosan nanoparticles and a chitosan nanoparticle–amoxicillin complex

Nguyen, Tan Vui; Nguyen, Thi Thu Ha; Wang, San-Lang; Vo, Thi Phuong Khanh; Nguyen, Anh Dzung

doi:10.1007/s11164-016-2428-8

Cited by 100 publications

(52 citation statements)

References 36 publications

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“…These results proved that F3 exhibited higher antimicrobial activity due to the combined effect of chitosan with the ciprofloxacin. This proves that ciprofloxacin with chitosan has better antimicrobial activity than the antibiotic alone, this agreed with Sobhani et al, who stated that CIP-CS nanoparticles decreased Minimum inhibitory concentration of gram positive and gram negative bacteria with 50% (Kishen et al, 2008;Nguyen et al, 2017;Sobhani et al, 2017). The higher the antibacterial activity of nanoparticles F3 may be due to interaction with the bacterial cell membrane, due to the positive charge of chitosan that adheres to the negative charge of the bacterial cell membrane.…”

Section: Antimicrobial Activity Using Disc Agar Diffusion Methodssupporting

confidence: 84%

“…Nanoparticles coated with chitosan showed that eradication of biofilm is applicable and this was in agreement with Ong et al, Kishen et al, and Shrestha et al (Kishen et al, 2008;Shrestha et al, 2010;Ong et al, 2017) they stated that using chitosan nanoparticles reduce biofilm and disrupt its structure. This reveals that nanoparticles are able to penetrate biofilm and eradicate bacteria (Avadi et al, 2004;Nguyen et al, 2017).…”

Section: Biofilm Inhibition Assaymentioning

confidence: 94%

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Preparation of PLGA-chitosan based nanocarriers for enhancing antibacterial effect of ciprofloxacin in root canal infection

2019

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The aim of this study is to prepare and evaluate the antibacterial and antibiofilm activity of ciprofloxacin (CIP) loaded PLGA nanoparticles (F2) and CIP-PLGA nanoparticles coated with chitosan (F3) versus ciprofloxacin solution (Fl) as a control on Enterococcus faecalis. F2 was prepared using double emulsion evaporation technique then coated with chitosan (F3). The prepared F2 and F3 were evaluated for size, surface charge, encapsulation efficiency, morphology and in vitro release. F1, F2, F3, and Chitosan (CS) were assessed in vitro using agar diffusion technique and biofilm inhibition assay. Finally, biofilm inhibition on teeth using Colony Forming Unit (CFU) was implemented with different concentrations of the three formulae. The results revealed that F2 is 202.9 nm with a negative charge À0.0254 mv, while F3 is 339.6 nm with a positive charge þ28.5 mv. The encapsulation efficiency of F2, and F3 was 64% and 78% respectively. The amount released was 92.62% and 78.3% for F2 and F3, respectively, after 72 h, while F1 showed 100% released in the first hour. CS, F1, F2, and F3, showed antibacterial effect with inhibition zone of 12 mm, 22 mm, 20 mm, and 32 mm respectively. Biofilm inhibition of F1, F2, and F3 were 60%, 74%, and 91.8%, respectively. F3 colony count was less than F2, and F1 in all concentrations. It can be concluded that F3 had proven to exhibit potential antibacterial and antibiofilm activity in a controlled release pattern consequently, they can be used as an intracanal medication.

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Section: Antimicrobial Activity Using Disc Agar Diffusion Methodssupporting

confidence: 84%

Section: Biofilm Inhibition Assaymentioning

confidence: 94%

Preparation of PLGA-chitosan based nanocarriers for enhancing antibacterial effect of ciprofloxacin in root canal infection

2019

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“…CNPs are usually made up by ionic gelation method, which describes the crosslinking reaction of CS with TPP [ 23 , 24 ]. Positively charged CS interacts with the negatively-charged crosslinker called sodium tripolyphosphate (TPP) [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

Synthesis and Optimization of Chitosan Nanoparticles Loaded with l-Ascorbic Acid and Thymoquinone

et al. 2018

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The combination of compounds with different classes (hydrophobic and hydrophilic characters) in single chitosan carrier is a challenge due to the hydrophilicity of chitosan. Utilization of l-ascorbic acid (LAA) and thymoquinone (TQ) compounds as effective antioxidants is marred by poor bioavailability and uptake. Nanoparticles (NPs) solved the problem by functioning as a carrier for them because they have high surface areas for more efficient delivery and uptake by cells. This research, therefore, synthesized chitosan NPs (CNPs) containing LAA and TQ, CNP-LAA-TQ via ionic gelation routes as the preparation is non-toxic. They were characterized using electron microscopy, zetasizer, UV–VIS spectrophotometry, and infrared spectroscopy. The optimum CNP-LAA-TQ size produced was 141.5 ± 7.8 nm, with a polydispersity index (PDI) of 0.207 ± 0.013. The encapsulation efficiency of CNP-LAA-TQ was 22.8 ± 3.2% for LAA and 35.6 ± 3.6% for TQ. Combined hydrophilic LAA and hydrophobic TQ proved that a myriad of highly efficacious compounds with poor systemic uptake could be encapsulated together in NP systems to increase their pharmaceutical efficiency, indirectly contributing to the advancement of medical and pharmaceutical sectors.

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“…Owning to the reactive amino side groups, chitosan could be made available via chemical modifications or ionic interactions [14]. Chitosan-bearing protonated amino groups could interact with a wide variety of natural or synthetic anionic species, such as negatively charged proteins, DNA [15–19], and some synthetic basic polymers such as sodium tripolyphosphate (TPP) [20–21] to form ionic complexes. This ionic gelation method to prepare Cs/TPP nanoparticles (CNs) with the advantages of simple operation, low equipment requirements, low cost, good repeatability, environmentally friendly, and easy large-scale preparation, has been extensively studied for obtaining nanocarrier systems with a good capacity of drug encapsulation and an adjustable drug release rate [22–23].…”

Section: Introductionmentioning

confidence: 99%

Chitosan-based nanoparticles for improved anticancer efficacy and bioavailability of mifepristone

Zhang¹,

Wu²,

Li³

et al. 2016

Beilstein J. Nanotechnol.

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In addition to its well-known abortifacient effect, mifepristone (MIF) has been used as an anticancer drug for various cancers in many studies with an in-depth understanding of the mechanism of action. However, application of MIF is limited by its poor water solubility and low oral bioavailability. In this work, we developed a drug delivery system based on chitosan nanoparticles (CNs) to improve its bioavailability and anticancer activity. The MIF-loaded chitosan nanoparticles (MCNs) were prepared by convenient ionic gelation techniques between chitosan (Cs) and tripolyphosphate (TPP). The preparation conditions, including Cs concentration, TPP concentration, Cs/MIF mass ratio, and pH value of the TPP solution, were optimized to gain better encapsulation efficiency (EE) and drug loading capacity (DL). MCNs prepared with the optimum conditions resulted in spherical particles with an average size of 200 nm. FTIR and XRD spectra verified that MIF was successfully encapsulated in CNs. The EE and DL of MCNs determined by HPLC were 86.6% and 43.3%, respectively. The in vitro release kinetics demonstrated that MIF was released from CNs in a sustained-release manner. Compared with free MIF, MCNs demonstrated increased anticancer activity in several cancer cell lines. Pharmacokinetic studies in male rats that were orally administered MCNs showed a 3.2-fold increase in the area under the curve from 0 to 24 h compared with free MIF. These results demonstrated that MCNs could be developed as a potential delivery system for MIF to improve its anticancer activity and bioavailability.

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Preparation of chitosan nanoparticles by TPP ionic gelation combined with spray drying, and the antibacterial activity of chitosan nanoparticles and a chitosan nanoparticle–amoxicillin complex

Cited by 100 publications

References 36 publications

Preparation of PLGA-chitosan based nanocarriers for enhancing antibacterial effect of ciprofloxacin in root canal infection

Preparation of PLGA-chitosan based nanocarriers for enhancing antibacterial effect of ciprofloxacin in root canal infection

Synthesis and Optimization of Chitosan Nanoparticles Loaded with l-Ascorbic Acid and Thymoquinone

Chitosan-based nanoparticles for improved anticancer efficacy and bioavailability of mifepristone

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