Preparation of Chitosan nanoparticles and its effect on detached rice leaves infected with Pyricularia grisea

Manikandan, A.; Sathiyabama, Muthukrishnan

doi:10.1016/j.ijbiomac.2015.11.083

Cited by 93 publications

(43 citation statements)

References 18 publications

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“…Early blight and Fusarium wilt of tomato were significantly reduced due to Cu-chitosan nanoparticles 7 . Blast disease of rice and finger millet were effectively suppressed by Chitosan nanoparticles 8 , 24 .…”

Section: Discussionmentioning

confidence: 99%

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Chitosan nanoparticles having higher degree of acetylation induce resistance against pearl millet downy mildew through nitric oxide generation

Siddaiah

Prasanth

Satyanarayana

et al. 2018

Sci Rep

116

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Downy mildew of pearl millet caused by the biotrophic oomycete Sclerospora graminicola is the most devastating disease which impairs pearl millet production causing huge yield and monetary losses. Chitosan nanoparticles (CNP) were synthesized from low molecular weight chitosan having higher degree of acetylation was evaluated for their efficacy against downy mildew disease of pearl millet caused by Sclerospora graminicola. Laboratory studies showed that CNP seed treatment significantly enhanced pearl millet seed germination percentage and seedling vigor compared to the control. Seed treatment with CNP induced systemic and durable resistance and showed significant downy mildew protection under greenhouse conditions in comparison to the untreated control. Seed treatment with CNP showed changes in gene expression profiles wherein expression of genes of phenylalanine ammonia lyase, peroxidase, polyphenoloxidase, catalase and superoxide dismutase were highly upregulated. CNP treatment resulted in earlier and higher expression of the pathogenesis related proteins PR1 and PR5. Downy mildew protective effect offered by CNP was found to be modulated by nitric oxide and treatment with CNP along with NO inhibitors cPTIO completely abolished the gene expression of defense enzymes and PR proteins. Further, comparative analysis of CNP with Chitosan revealed that the very small dosage of CNP performed at par with recommended dose of Chitosan for downy mildew management.

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Section: Discussionmentioning

confidence: 99%

“…Cu-chitosan nanoparticles effectively controlled tomato early blight and Fusarium wilt 7 . Chitosan nanoparticles effectively suppressed of rice and finger millet blast fungus Pyricularia grisea 8 , 9 .…”

Section: Introductionmentioning

confidence: 99%

Chitosan nanoparticles having higher degree of acetylation induce resistance against pearl millet downy mildew through nitric oxide generation

Siddaiah

Prasanth

Satyanarayana

et al. 2018

Sci Rep

116

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show abstract

“…More recently, nanoparticles of CHT and sodium tripolyphosphate were prepared and successfully evaluated for suppression of rice blast fungus ( Pyricularia grisea ) [ 117 ] and oleoyl-chitosan nanoparticles were synthesized and used to disperse antifungal products [ 118 ].…”

Section: Use Of Cht Nanoparticles In Agriculturementioning

confidence: 99%

Chitosan Effects on Plant Systems

Malerba

Cerana

2016

IJMS

353

210

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Chitosan (CHT) is a natural, safe, and cheap product of chitin deacetylation, widely used by several industries because of its interesting features. The availability of industrial quantities of CHT in the late 1980s enabled it to be tested in agriculture. CHT has been proven to stimulate plant growth, to protect the safety of edible products, and to induce abiotic and biotic stress tolerance in various horticultural commodities. The stimulating effect of different enzyme activities to detoxify reactive oxygen species suggests the involvement of hydrogen peroxide and nitric oxide in CHT signaling. CHT could also interact with chromatin and directly affect gene expression. Recent innovative uses of CHT include synthesis of CHT nanoparticles as a valuable delivery system for fertilizers, herbicides, pesticides, and micronutrients for crop growth promotion by a balanced and sustained nutrition. In addition, CHT nanoparticles can safely deliver genetic material for plant transformation. This review presents an overview on the status of the use of CHT in plant systems. Attention was given to the research that suggested the use of CHT for sustainable crop productivity.

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“…The value of PI is an index of the size distribution of nanoparticles and a value less than 0.1 indicates a homogeneous population of particles. The differences in size between DLS and SEM measurements were probably due to the different principles in the function of these instruments [13] and also to the different pretreatments that the samples undergo. Moreover, it is important to underline that SEM measurement is mostly used to verify nanoparticle shape and morphology, while DLS analysis is used as a prediction of the average size and polydispersity index of nanoparticles [14].…”

Section: Morphological Characterization Of Chi-dhar Nanoparticlesmentioning

confidence: 99%

Controlled Release of 5-FU from Chi–DHA Nanoparticles Synthetized with Ionic Gelation Technique: Evaluation of Release Profile Kinetics and Cytotoxicity Effect

Ruffo

Parisi

Patitucci

et al. 2020

JFB

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The ionic gelation technique allows us to obtain nanoparticles able to function as carriers for hydrophobic anticancer drugs, such as 5-fluoruracil (5-FU). In this study, reticulated chitosan– docosahexaenoic acid (Chi–DHAr) nanoparticles were synthesized by using a chemical reaction between amine groups of chitosan (Chi) and carboxylic acids of docosahexaenoic acid (DHA) and the presence of a link between Chi and DHA was confirmed by FT-IR, while the size and morphology of the obtained Chi-DHAr nanoparticles was evaluated with dynamic light scattering (DLS) and scanning electron microscopy (SEM), respectively. Drug-loading content (DLC) and drug-loading efficiency (DLE) of 5-FU in Chi-DHAr nanoparticles were 33.74 ± 0.19% and 7.9 ± 0.26%, respectively, while in the non-functionalized nanoparticles (Chir + 5FU), DLC, and DLE were in the ranges of 23.73 ± 0.14%, 5.62%, and 0.23%, respectively. The in vitro release profile, performed in phosphate buffer saline (PBS, pH 7.4) at 37 °C, indicated that the synthetized Chi–DHAr nanoparticles provided a sustained release of 5-FU. Based on the obtained regression coefficient value (R2), the first order kinetic model provided the best fit for both Chir and Chi-DHAr nanoparticles. Finally, cytotoxicity studies of chitosan, 5-FU, Chir, Chir + 5-FU, Chi-DHAr, and Chi-DHAr + 5-FU nanoparticles were conducted. Overall, Chi-DHAr nanoparticles proved to be much more biocompatible than Chir nanoparticles while retaining the ability to release the drug with high efficiency, especially towards specific types of cancerous cells.

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Preparation of Chitosan nanoparticles and its effect on detached rice leaves infected with Pyricularia grisea

Cited by 93 publications

References 18 publications

Chitosan nanoparticles having higher degree of acetylation induce resistance against pearl millet downy mildew through nitric oxide generation

Chitosan nanoparticles having higher degree of acetylation induce resistance against pearl millet downy mildew through nitric oxide generation

Chitosan Effects on Plant Systems

Controlled Release of 5-FU from Chi–DHA Nanoparticles Synthetized with Ionic Gelation Technique: Evaluation of Release Profile Kinetics and Cytotoxicity Effect

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