Preparation of chitosan and its miscibility studies with gelatin using viscosity, ultrasonic and refractive index

Basavaraju, K. C.; Damappa, T.; Rai, S.K.

doi:10.1016/j.carbpol.2006.03.020

Cited by 28 publications

(10 citation statements)

References 11 publications

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“…Previously chitosan-gelatin composite formation has been studied by Chen et al (2003), Basavaraju et al (2006), and Mi (2005) and its application for food products had been explored by Arvanitoyannis et al (1998) and Lo'pez-Caballero et al (2005), but this bio-composite had never been investigated to be used as a matrix for tyrosinase immobilization. However, several workers have endeavored to immobilize tyrosinase onto alginate, polyacrylamide and gelatin gels (Munjal and Sawhney, 2002), ZnO nanoparticles (Li et al, 2006), cellulose support (Labus et al, 2011) and polystyrene microplate (Saini et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Aspergillus niger PA2 Tyrosinase Covalently Immobilized on a Novel Eco-Friendly Bio-Composite of Chitosan-Gelatin and Its Evaluation for L-DOPA Production

et al. 2016

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Tyrosinase (EC 1.14.18.1) a copper-containing monooxygenase, isolated from a fungal isolate Aspergillus niger PA2 was subjected for immobilization onto a composite consisting of chitosan and gelatin biopolymers. The homogeneity of the chitosan-gelatin biocomposite film was characterized by X-ray diffraction analyses. To evaluate immobilization efficiency, chitosan-gelatin-Tyr bio-composite films were analyzed by field emission scanning electron microscopy, atomic force microscopy and UV-spectroscopy. The rough morphology of the film led to a high loading of enzyme and it could retain its bioactivity for a longer period. The enzyme adsorbed onto the film exhibited 72% of its activity after 10 days and exhibited good repeatability for up to nine times, after intermittent storage. Moreover, the immobilized enzyme exhibited broader pH and temperature profile as compared to free counterpart. Immobilized enzyme was further evaluated for the synthesis of L-DOPA (2,4-dihydroxy phenylalanine) which is a precursor of dopamine and a potent drug for the treatment of Parkinson's disease and for myocardium neurogenic injury.

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Section: Introductionmentioning

confidence: 99%

Aspergillus niger PA2 Tyrosinase Covalently Immobilized on a Novel Eco-Friendly Bio-Composite of Chitosan-Gelatin and Its Evaluation for L-DOPA Production

et al. 2016

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“…Chitosan is derived from chitin by deacetylation [1,2]. Chitosan films have many commercial and biomedical uses [3,4].…”

Section: Introductionmentioning

confidence: 99%

Comparison of chitosan/starch composite film properties before and after cross-linking

Gao

Wang

et al. 2013

International Journal of Biological Macromolecules

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“…Chitosan as one of the most favorable thermosensitive hydrogel materials has recently attracted attention in pharmaceutical and biomedical fields 15–18. Gelatin is a heterogeneous mixture of hot water‐soluble proteins of high‐average molecular weight, obtained by the thermal denaturation of collagen 19. Gelatin contains carboxyl groups on its chain backbones and has the potential to mix with chitosan because of its ability to form hydrogen bonding with chitosan 20.…”

Section: Introductionmentioning

confidence: 99%

Preparation and characterization of a novel thermosensitive hydrogel based on chitosan and gelatin blends

Chang

Xiao

Tang

2009

J of Applied Polymer Sci

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In this study, a novel injectable in situ gelling thermosensitive hydrogel system based on chitosan and gelatin blends was designed and investigated. The addition of gelatin provides the correct buffering and other physicochemical conditions including control of hydrophobic interactions and hydrogen bonding, which are necessary to retain chitosan in solution at neutral pH near 4 C and furthermore to allow gel formation upon heating to body temperature. The chitosan/gelatin hydrogels were studied by FTIR, swelling, and rheological analysis. The rheological analysis evidenced the endothermic gelation of chitosan/gelatin solutions, which indicated their gelation temperatures and reflected the effect of gelatin concentration on the thermosensitive properties of gels.The morphology of this system was examined with laser scanning confocal microscopy and scanning electron microscopy. The images indicated that the gels were quite heterogeneous and porous. The investigation of these gels as vehicles for delivering bovine serum albumin as a model drug of protein showed that the system could sustain the release of the protein drug. These results show that chitosan/gelatin solutions can form gels rapidly at body temperature and have promising perspective for their use in local and sustained delivery of protein drug.

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Preparation of chitosan and its miscibility studies with gelatin using viscosity, ultrasonic and refractive index

Cited by 28 publications

References 11 publications

Aspergillus niger PA2 Tyrosinase Covalently Immobilized on a Novel Eco-Friendly Bio-Composite of Chitosan-Gelatin and Its Evaluation for L-DOPA Production

Aspergillus niger PA2 Tyrosinase Covalently Immobilized on a Novel Eco-Friendly Bio-Composite of Chitosan-Gelatin and Its Evaluation for L-DOPA Production

Comparison of chitosan/starch composite film properties before and after cross-linking

Preparation and characterization of a novel thermosensitive hydrogel based on chitosan and gelatin blends

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