Preparation of chitooligosaccharides with degree of polymerization higher than 6 by acid or enzymatic degradation of chitosan

Cabrera, Juan Carlos; Cutsem, Pierre Van

doi:10.1016/j.bej.2005.04.025

Cited by 172 publications

(121 citation statements)

References 21 publications

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“…Peaks corresponding to hepta-and octa-oligomers were detected at much lower intensities. These results were confirmed by silica TLC analysis (Cabrera and Van Cutsem 2005).…”

Section: Preparation Of Chitooligosaccharides Of Different Dp and Dasupporting

confidence: 76%

“…However, it is widely accepted that the relative ion intensity corresponding to one oligomer reflects the relative quantity of this oligomer in the mixture (Akiyama et al 1995. As reported previously, selective precipitation of hydrolysis products in the presence of 90% methanol reduced the proportion of low DP oligomers (Cabrera and Van Cutsem 2005).…”

Section: Preparation Of Chitooligosaccharides Of Different Dp and Damentioning

confidence: 92%

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Size, acetylation and concentration of chitooligosaccharide elicitors determine the switch from defence involving PAL activation to cell death and water peroxide production in Arabidopsis cell suspensions

Cabrera

Messiaen

Cambier

et al. 2006

Physiologia Plantarum

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Chitosan oligomers are known elicitors of plant defence mechanisms. In this work, chitooligosaccharides of different degrees of polymerization and degrees of acetylation were prepared and characterized by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. The effect of the degree of polymerization (DP), degree of acetylation and concentration of these chitooligosaccharides on defence activation in Arabidopsis thaliana suspension-cultured cells was studied. Our study results show that fully deacetylated chitooligosaccharides (chitosan oligomers) induce, depending on their DP and concentration, phenylalanine ammonia-lyase (PAL) activation, H 2 O 2 synthesis and cell death in A. thaliana cell suspensions. The progressive reacetylation of the chitosan oligomer elicitors progressively impaired their ability to enhance H 2 O 2 accumulation and cell death, but did not affect the activation of PAL.

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“…Peaks corresponding to hepta-and octa-oligomers were detected at much lower intensities. These results were confirmed by silica TLC analysis (Cabrera and Van Cutsem 2005).…”

Section: Preparation Of Chitooligosaccharides Of Different Dp and Dasupporting

confidence: 76%

Section: Preparation Of Chitooligosaccharides Of Different Dp and Damentioning

confidence: 92%

Size, acetylation and concentration of chitooligosaccharide elicitors determine the switch from defence involving PAL activation to cell death and water peroxide production in Arabidopsis cell suspensions

Cabrera

Messiaen

Cambier

et al. 2006

Physiologia Plantarum

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“…COS formed by acid hydrolysis of chitosan was characterized by Thin Layer Chromatography (TLC). Each mean value is average of three replicates TLC method: 1µl of the acid hydrolysate was taken and spotted on the TLC silica gel 60 F254 5×10 aluminum plate (M/s Merck, USA) and solvent phase containing n-propanol : water : 25% ammonia water (7:2:1) (Carbrera and Cutsemb 2005). Hydrolysate was spotted against the standard glucosamine in parallel.…”

Section: Characterization Of Extracted Chitin and Chitooligosaccharidesmentioning

confidence: 99%

Extraction and characterization of chitin, chitosan and chitooligosaccharides from crab shell waste

Varun

Senani

Kumar

et al. 2017

IJAR

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An experiment was carried out to utilize the crab shell waste for the production of chitin by chemical extraction method. The obtained chitin was processed to make the more useful compound chitosan by deacetylation of chitin using strong NaOH solution at high temperature. Yield of chitin and chitosan from crab shell waste was found to be 12.2 % and 10.54 %, respectively. Chitosan was further used to produce chito-oligosaccharides (COS) by acid hydrolysis using 0.5 N H 2 SO 4 , 1N H 2 SO 4 and 7N HCl at hourly intervals upto 6 hours and the average yield was 0.55, 0.84 and 1.93 mg/ml, respectively. Chitin and chitosan were characterized by Fourier Transform Infrared spectroscopy (FT-IR) technique which depicts different band representing various bonds present in the molecule and which were in agreement with the bands of standard chitin and chitosan. Further, COS were formed after acid hydrolysis of chitosan, which was characterized by Thin Layer Chromatography (TLC) using glucosamine as a standard.

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“…According to previous determinations (Cabrera and Van Cutsem, 2005), the degree of acetylation of this chitosan is 12% and its molecular weight 82.9 kDa. The chitosan polymer powder was then stirred for 2 h (to achieve dissolution) in 1% acetic acid before adjusting the pH to 5.6 by adding 2N KOH.…”

Section: Chitosan Preparationmentioning

confidence: 99%

Evaluation of chitosan as an inhibitor of soil-borne pathogens and as an elicitor of defence markers and resistance in tobacco plants

Rodríguez

Menéndez

Delgado

et al. 2007

Span. j. agric. res.

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In in vitro assays, a chitosan polymer caused differential growth inhibition of the following pathogens isolated from tobacco: Phytophthora parasitica Dastur var. nicotianae (Ppn), Pythium aphanidermatum (Edson) Fitzp, Rhizoctonia solani Kühn, and Sclerotium rolfsii Sacc. The most sensitive were P. aphanidermatum and S. rolfsii, the growth of which was fully inhibited at a chitosan dose of 1.5 g L -1 ; the growth of Ppn was fully inhibited at 2 g L -1 . In vivo assays involving plants grown from seeds immersed in chitosan, as well as plants sprayed with this product, were performed to detect the induction of defence response markers in the leaf and consequent resistance to disease. Although defence/resistance marker enzyme activities varied, activation was greater in the chitosan-treated plants than in controls. Marker enzyme activities in the sprayed plants were generally equal to or stronger than those recorded in the seed immersion-treated plants, except for phenylalanine ammonia-lyase activity at the lowest immersion concentration tested. Although there were no statistical differences among treatments with respect to resistance against Ppn, the greatest protection was afforded by the spray treatments, in which the infection index was reduced between 17 and 19% compared to the controls. In conclusion, this chitosan polymer directly inhibited the growth of several tobacco pathogens and caused the induction of defence enzymes in leaves, but was not able to protect tobacco plantlets against Ppn infection via the activation of induced resistance. This work demonstrated the potential of chitosan in protecting tobacco plants against soil-borne pathogens.Additional key words: antifungal activity, induced resistance, Nicotiana tabacum, Phytophthora parasitica, Pythium aphanidermatum, Rhizoctonia solani, Sclerotium rolfsii. Resumen Evaluación de quitosano como inhibidor de patógenos del suelo y elicitor de marcadores defensivos y resistencia en plantas de tabacoEn ensayos in vitro, se demostró la inhibición diferenciada, causada por un polímero de quitosano, sobre el crecimiento de los siguientes patógenos del suelo aislados de tabaco: Phytophthora parasitica Dastur var. nicotianae (Ppn), Pythium aphanidermatum (Edson) Fitzp, Rhizoctonia solani Kühn y Sclerotium rolfsii Sacc. Los más sensibles fueron P. aphanidermatum y S. rolfsii, ya que la inhibición total del crecimiento ocurrió con 1,5 g L -1 de quitosano, mientras el crecimiento de Ppn fue totalmente inhibido a 2 g L -1 . En ensayos in vivo, se determinó la inducción foliar de marcadores defensivos y resistencia en plántulas de tabaco previamente tratadas por inmersión de semillas y aspersión foliar del quitosano. La activación enzimática fue mayor en los tratamientos con quitosano. Al analizar las dos

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Preparation of chitooligosaccharides with degree of polymerization higher than 6 by acid or enzymatic degradation of chitosan

Cited by 172 publications

References 21 publications

Size, acetylation and concentration of chitooligosaccharide elicitors determine the switch from defence involving PAL activation to cell death and water peroxide production in Arabidopsis cell suspensions

Size, acetylation and concentration of chitooligosaccharide elicitors determine the switch from defence involving PAL activation to cell death and water peroxide production in Arabidopsis cell suspensions

Extraction and characterization of chitin, chitosan and chitooligosaccharides from crab shell waste

Evaluation of chitosan as an inhibitor of soil-borne pathogens and as an elicitor of defence markers and resistance in tobacco plants

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