Preparation of Chitooligosaccharide by Hydrogen Peroxide Degradation of Chitosan and Its Effect on Soybean Seed Germination

Hai, Nguyen Thi Thanh; Thu, Le Hoai; Nga, Nguyễn Thị Thanh; Hòa, Trần Thái; Tuan, Le Nghiem Anh; Phú, Đặng Văn; Hiến, Nguyễn Quốc

doi:10.1007/s10924-019-01479-y

Cited by 23 publications

(14 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…10 %) increased the germination rate of soybean seeds (Hai et al, 2019). Several genes involved in isoflavone accumulation, including phenylalanine ammonia-lyase (PAL) and isoflavone synthase (IFS), were up-regulated and the contents of flavonoid and total phenolics were increased during soybean seed germination upon treatment with chitosan oligomers (DP 3-7, DA unknown) (Jia et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Effect of chitosan foliar application on the performance and yield of soybean and maize under field conditions.

Albrecht

Villetti

et al. 2021

agriRxiv

View full text Add to dashboard Cite

Chitosan is a natural heterogeneous polymer, non-toxic and find many potential applications in agriculture. The search of a biopolymer to replace the synthetic agrochemicals, the effects of foliar application of chitosan solution on agronomic performance under field experiments have been investigated in two consecutive harvests (2014/2015 and 2015) of soybean and maize. Two chitosan concentrations (0.5 and 1%) were sprayed four times during the culture development and the evaluation was based on morphological characteristics, growth and yield components. Seeds were collected to determine the content of macro- and micronutrients. Results revealed that most of the morphological and growth parameters (plant height, leaf number per plant) and yield attributes were not increased with the chitosan treatment in soybean and maize. The soybean treated with foliar application of chitosan 0.5% increased the accumulation of nitrogen by 9.14% when compared with the water treatment, but the effect was not consistent in both seasons and cultivars. The values of the other nutrients did not change the content. Maize plants treated with chitosan (0.5% or 1%) significantly decreased the boron concentration by 66.7% in the grains analyzed and may have influenced the plant development (height) without altering the other agronomic parameters. Therefore, whereas the climatic conditions in these two seasons were stable and biotic or abiotic stresses were not observed, foliar application of chitosan solution did not present differences that could establish a pattern of behavior in field experiments conducted by two harvests.

show abstract

Section: Discussionmentioning

confidence: 99%

Effect of chitosan foliar application on the performance and yield of soybean and maize under field conditions.

Albrecht

Villetti

et al. 2021

agriRxiv

View full text Add to dashboard Cite

show abstract

“…However, it remains difficult to scale up the production of OCS using physical methods due to the lack of corresponding facilities [ 20 ]. On the other hand, the chemical approach, such as oxidative degradation of chitosan using hydrogen peroxide (H 2 O 2 ), which can be used for large-scale production of OCS at a reasonable cost, has emerged as a promising alternative and received a great research interest [ 21 , 22 , 23 ]. Nevertheless, the relative molecular weight of OCS produced by this method is widely distributed, thus, requires intensive post-treatment to separate and purify the products [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

“…For both economic and environmental benefits, the use of local resources or bio-waste of the crustacean processing for the production of OCS emerged as a promising and sustainable solution. Several studies in Vietnam attempted to synthesize OCS using different approaches such as H 2 O 2 degradation and gamma irradiation combined with H 2 O 2 treatment for mainly agricultural applications [ 18 , 19 , 21 , 28 ]. Nevertheless, studies utilizing the local chitin source to produce OCS for infectious wound management purposes are lacking.…”

Section: Introductionmentioning

confidence: 99%

Characterizations and Antibacterial Efficacy of Chitosan Oligomers Synthesized by Microwave-Assisted Hydrogen Peroxide Oxidative Depolymerization Method for Infectious Wound Applications

Doan

Tran

et al. 2021

Materials

View full text Add to dashboard Cite

The use of naturally occurring materials with antibacterial properties has gained a great interest in infected wound management. Despite being an abundant resource in Vietnam, chitosan and its derivatives have not yet been intensively explored for their potential in such application. Here, we utilized a local chitosan source to synthesize chitosan oligomers (OCS) using hydrogen peroxide (H2O2) oxidation under the microwave irradiation method. The effects of H2O2 concentration on the physicochemical properties of OCS were investigated through molecular weight, degree of deacetylation, and heavy metal contamination for optimization of OCS formulation. Then, the antibacterial inhibition was examined; the minimum inhibitory concentration and minimum bactericidal concentration (MIC and MBC) of OCS-based materials were determined against common skin-inhabitant pathogens. The results show that the local Vietnamese chitosan and its derivative OCS possessed high-yield purification while the molecular weight of OCS was inversely proportional and proportional to the concentration of H2O2, respectively. Further, the MIC and MBC of OCS ranged from 3.75 to less than 15 mg/mL and 7.5–15 mg/mL, respectively. Thus, OCS-based materials induce excellent antimicrobial properties and can be attractive for wound dressings and require further investigation.

show abstract

“…In fact, chitosan dissolved in different acids can be degraded into corresponding salt form chitooligosaccharides. For example, Hai et al [16] dissolved chitosan in lactic acid and degraded chitosan with hydrogen peroxide to obtain chitooligosaccharide lactate. 1 HNMR showed that the product of chitooligosaccharide lactate prepared by Hai et al has one more hydrogen absorption peak in the methyl group than chitosan.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Different Salt Forms of Chitooligosaccharides and Their Effects on Nitric Oxide Secretion by Macrophages

Xing

Gao

et al. 2021

Molecules

View full text Add to dashboard Cite

In this paper, chitooligosaccharides in different salt forms, such as chitooligosaccharide lactate, citrate, adipate, etc., were prepared by the microwave method. They were characterized by SEM, FTIR, NMR, etc., and the nitric oxide (NO) expression was determined in RAW 264.7 cells. The results showed that pure chitooligosaccharide was an irregular spherical shape with rough surface, and its different salt type products are amorphous solid with different honeycomb sizes. In addition to the characteristic absorption peaks of chitooligosaccharides, in FTIR, the characteristic absorption of carboxyl group, methylene group, and aromatic group in corresponding acid appeared. The characteristic absorption peaks of carbon in carboxyl group, hydrogen and carbon in methyl, methylene group, and aromatic group in corresponding acid also appeared in NMR. Therefore, the sugar ring structure and linking mode of chitooligosaccharides did not change after salt formation of chitooligosaccharides. Different salt chitooligosaccharides are completely different in promoting NO secretion by macrophages, and pure chitooligosaccharides are the best.

show abstract

Preparation of Chitooligosaccharide by Hydrogen Peroxide Degradation of Chitosan and Its Effect on Soybean Seed Germination

Cited by 23 publications

References 34 publications

Effect of chitosan foliar application on the performance and yield of soybean and maize under field conditions.

Effect of chitosan foliar application on the performance and yield of soybean and maize under field conditions.

Characterizations and Antibacterial Efficacy of Chitosan Oligomers Synthesized by Microwave-Assisted Hydrogen Peroxide Oxidative Depolymerization Method for Infectious Wound Applications

Characterization of Different Salt Forms of Chitooligosaccharides and Their Effects on Nitric Oxide Secretion by Macrophages

Contact Info

Product

Resources

About