Preparation of Chicken Anemia Virus (CAV) Virus-Like Particles and Chicken Interleukin-12 for Vaccine Development Using a Baculovirus Expression System

Tseng, Ta-Yuan; Liu, Yee-Chen; Hsu, Yu‐Chen; Chang, Poa-Chun; Hsieh, Ming-Kun; Shien, Jui-Hung; Ou, Shan-Chia

doi:10.3390/pathogens8040262

Cited by 13 publications

(12 citation statements)

References 38 publications

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“…In addition, the safety of a potential vaccine is closely related to the health of chickens ( 24 ). Here, we investigated the extent of CIAV contamination in the live attenuated non-CIAV vaccines sold at market in Henan Province.…”

Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology and Pathogenic Characterization of Novel Chicken Infectious Anemia Viruses in Henan Province of China

et al. 2022

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Chicken infectious anemia (CIA) is an immunosuppressive disease caused by the chicken infectious anemia virus (CIAV) resulting in heavy economic losses once an outbreak is established. This study conducted a systematic analysis of the epidemiology and pathology of CIA in Henan province, China. A total of 437 clinical tissue samples and 120 poultry disease-related live attenuated vaccines were collected during 2017–2020; of which 45 were positive for CIAV nucleic acid, with a positive rate of 8.08%. Our results showed that genome sequence similarity among a total of 12 CIAV isolates was high, and ranged from 97.1 to 99.3%, and their similarity to the vaccine strains Cux-1 and Del-Ros ranged from 97.8 to 98.6%. However, There were mutations in the locus of the major capsid proteins VP1, VP2, and VP3 among all isolates. The subsequent sequence analysis indicated that the isolates of HN-4 and HN-8 showed genetic recombination and follow up animal experiments revealed that HN-4 might be a pathogenic strain. Our results reveal that both field infection and non-CIAV vaccines contamination promote the epidemiology of CIAV in China and some dominant epidemic viruses have undergone recombination and evolution. This study provides important information to help with the prevention and control of CIAV in the poultry industry.

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Section: Discussionmentioning

confidence: 99%

Molecular Epidemiology and Pathogenic Characterization of Novel Chicken Infectious Anemia Viruses in Henan Province of China

et al. 2022

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“…Vaccine persistence, circulation and reversion to virulence have also been frequently reported for other empirically attenuated avian vaccines developed to control immunosuppressive or respiratory diseases [ 52 , 53 , 54 ]. Next-generation vaccines, with improved stability and safety, are currently under study and development for most avian pathogens and, in the future, they will likely replace traditional vaccines [ 55 , 56 , 57 , 58 ].…”

Section: Discussionmentioning

confidence: 99%

Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl

Quaglia

Mescolini

Catelli

et al. 2021

Animals

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Chicken infectious anemia virus (CIAV) is a pathogen of chickens associated with immunosuppression and with a disease named chicken infectious anemia. The present survey reports an epidemiological study on CIAV distribution in Italian broiler, broiler breeder and backyard chicken flocks. Twenty-five strains were detected by a specifically developed nested PCR protocol, and molecularly characterized by partial VP1 gene or complete genome sequencing. Viral DNA amplification was successfully obtained from non-invasive samples such as feathers and environmental dust. Sequence and phylogenetic analysis showed the circulation of field or potentially vaccine-derived strains with heterogeneous sequences clustered into genogroups II, IIIa, and IIIb. Marker genome positions, reported to be correlated with CIAV virulence, were evaluated in field strains. In conclusion, this is the first survey focused on the molecular characteristics of Italian CIAVs, which have proved to be highly heterogeneous, implementing at the same time a distribution map of field viruses worldwide.

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“…In addition, the safety of a potential vaccine is closely related to the health of chickens [21]. Here, we investigated the extent of CIAV contamination in the live attenuated vaccines sold at market in Henan Province.…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of Isolated Chicken Infectious Anemia Viruses in Central China

Wang¹,

Feng²,

Liu³

et al. 2021

Preprint

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Background: Chicken infectious anemia (CIA) is an immunosuppressive disease induced by the chicken infectious anemia virus (CIAV) causing heavy economic losses once outbreak. This study conducted a systematic analysis of the epidemiology and pathology of CIA in Henan province, central China.Methods: A total of 437 clinical tissue samples and 120 poultry disease-related live attenuated vaccines were collected during 2017-2019; of which 45 were positive for CIAV nucleic acid, with a positive rate of 8.08%. Results: Our results showed that genome sequences similarity among a total of 12 CIAV isolates was high, and ranged from 97.1% to 99.3%, and their similarity to the vaccine strains Cux-1 and Del-Ros ranged from 97.8% to 98.6%. However, There were non-synonymous amino acid mutations in the locus of the major capsid proteins VP1, VP2 and VP3 among all isolates. The subsequent sequence analysis indicated that the isolates of HN-4 and HN-8 showed genetic recombination and follow up animal experiments revealed that HN-4 might be a high pathogenic strain. Conclusions: Our results reveal that both field infection and vaccine contamination promote epidemiology of CIAV in China. Some dominant epidemic viruses have undergone recombination and evolution. This study provides important information for CIAV prevention and control in poultry industry development.

show abstract

Preparation of Chicken Anemia Virus (CAV) Virus-Like Particles and Chicken Interleukin-12 for Vaccine Development Using a Baculovirus Expression System

Cited by 13 publications

References 38 publications

Molecular Epidemiology and Pathogenic Characterization of Novel Chicken Infectious Anemia Viruses in Henan Province of China

Molecular Epidemiology and Pathogenic Characterization of Novel Chicken Infectious Anemia Viruses in Henan Province of China

Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl

Molecular Characterization of Isolated Chicken Infectious Anemia Viruses in Central China

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