Preparation of canine C‐reactive protein serum reference material: A feasibility study

Canalías, Francesca; Piñeiro, Matilde; Pato, Raquel; Peña, Ruth; Bosch, Lluís; Soler, Lourdes; García, Natalia; Lampreave, Fermı́n; Saco, Yolanda; Bassols, Anna

doi:10.1111/vcp.12574

Cited by 3 publications

(7 citation statements)

References 34 publications

(76 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results of this study show that the new turbidimetric immunoassay developed by our group is a robust and accurate method that can be easily adapted to different clinical chemistry analyzers, thus providing a very useful tool for clinicians. This benefit is further validated by the good agreement of results obtained at different laboratories across Europe using the assay with different clinical chemistry analyzers …”

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

“…| 133 obtained at different laboratories across Europe using the assay with different clinical chemistry analyzers. 27 The use of specific reagents is fundamental for the development of robust and accurate species-specific turbidimetric immunoassays.…”

Section: Discussionmentioning

confidence: 99%

“…These discrepancies are probably mainly due to the different standards used for calibration and point out the necessity of using appropriate reference materials for assay calibration to harmonize the results among laboratories. Our group has addressed this question and studies to develop reference material for harmonization of cCRP assays have been carried out 27. In conclusion, our new particle-enhanced turbidimetric immunoassay is a robust, accurate, and precise method, which represents a good alternative for the routine analysis of cCRP in laboratories where a clinical chemistry analyzer is available.…”

mentioning

confidence: 99%

See 3 more Smart Citations

A new automated turbidimetric immunoassay for the measurement of canine C‐reactive protein

Piñeiro¹,

Pato

Soler

et al. 2018

Veterinary Clinical Pathol

Self Cite

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 85%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

A new automated turbidimetric immunoassay for the measurement of canine C‐reactive protein

Piñeiro¹,

Pato

Soler

et al. 2018

Veterinary Clinical Pathol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Overall, surgical site infection rates in small animals range from 2.83 to 5.8% [1–3]. C-reactive protein (CRP) is a powerful biomarker for inflammation, infection and sepsis, and is widely used in both human and veterinary medicine [4]. Clinical serum canine CRP (c-CRP) measurement has been performed for over 30 years [5], and increased serum c-CRP concentrations are known to occur with several types of infection [6–8].…”

Section: Resultsmentioning

confidence: 99%

Changes in canine C-reactive protein levels following orthopaedic surgery: a prospective study

et al. 2019

View full text Add to dashboard Cite

C-reactive protein (CRP) is a powerful biomarker for inflammation, infection and sepsis. However, no reports have investigated canine CRP (c-CRP) concentration changes after orthopaedic procedures. If c-CRP changes were better characterized, it may be possible to identify postoperative complications more quickly. The purpose of this study was to clarify the characteristic changes in serum c-CRP after orthopaedic surgery in dogs. Blood samples were collected from 98 dogs on Day 0 (preoperatively), and then on Days 1, 4, 7, 10 and 13 postoperatively. Day 1 blood sampling was performed 12–24 h postoperatively. We classified the dogs into four groups based on changes in c-CRP pre- to postoperatively. Group 1 dogs showed a peak c-CRP concentration on Day 1, followed by decreases of ≥ 1 mg/dL. Group 2 dogs showed changes in c-CRP concentration by Day 4 that were within ± 1 mg/dL compared with Day 1. Dogs in Group 3 showed a peak c-CRP concentration on Day 4, followed by decreases of ≥ 1 mg/dL. Group 4 dogs showed an initial decrease in c-CRP, then an increase of ≥ 1 mg/dL. Group 1 was the largest group, with 63 dogs. c-CRP on Days 0, 1, 4, 7, 10 and 13 was 0.83 ± 1.03 mg/dL, 8.10 ± 3.15 mg/dL, 3.76 ± 1.94 mg/dL, 1.63 ± 0.92 mg/dL, 0.96 ± 0.70 mg/dL and 0.68 ± 0.51 mg/dL, respectively. Serum c-CRP concentration on Day 1 was significantly higher than at every other timepoint (P < 0.001). In Group 2, surgical site complications were confirmed in 9/15 dogs. In Group 3, surgical site complications were confirmed in 7/14 dogs. In Group 4, two surgical site problems and three surgical site infections were observed, and visceral disease was found in one dog. In Group 1, peak c-CRP was seen on Day 1 postoperatively in 63 dogs (64%), with c-CRP level decreasing by half at each subsequent measurement, which may describe a typical c-CRP change in orthopaedic patients. If deviation from this typical change is observed postoperatively, as in Groups 2–4, this may suggest possible complications.

show abstract

Acute phase proteins in cattle and swine: A review

Saco

Bassols

2022

Veterinary Clinical Pathol

Self Cite

View full text Add to dashboard Cite

The major acute phase proteins (APPs) in cattle are haptoglobin (Hp) and serum amyloid A (SAA), and in swine, are Hp, SAA, C‐reactive protein (CRP), and Pig major acute phase protein (Pig‐MAP). Many methodologic assays are presently available to measure these parameters, which are still being improved to increase their specificity, sensitivity, user‐friendliness, and economic availability. In cattle, the main applications are the diagnosis and monitoring of frequent diseases such as mastitis and metritis in dairy cows and respiratory problems in young calves. In pigs, APPs are useful in the control of bacterial and viral infections, and they may be used at the slaughterhouse to monitor subclinical pathologies and improve food safety. The utility of APP in animal production must not be forgotten; optimization of protocols to improve performance, welfare, and nutrition may benefit from the use of APPs. Other sample types besides serum or plasma have potential uses; APP determination in milk is a powerful tool in the control of mastitis, saliva is a non‐invasive sample type, and meat juice is easily obtained at the slaughterhouse. Increasing our knowledge of reference intervals and the influence of variables such as age, breed, sex, and the season is important. Finally, worldwide harmonization and standardization of analytical procedures will help to expand the use of APPs.

show abstract

Preparation of canine C‐reactive protein serum reference material: A feasibility study

Abstract: Canine C-reactive protein serum reference material has been produced that allows harmonization of results obtained by different methods and different laboratories, thus reducing the possibility of errors and misunderstandings.

Cited by 3 publications

References 34 publications

A new automated turbidimetric immunoassay for the measurement of canine C‐reactive protein

A new automated turbidimetric immunoassay for the measurement of canine C‐reactive protein

Changes in canine C-reactive protein levels following orthopaedic surgery: a prospective study

Acute phase proteins in cattle and swine: A review

Contact Info

Product

Resources

About