Preparation of blood components with saline‐adenine‐glucose‐phosphate‐ maltose quadruple‐pack system

Abstract

A saline-adenine-glucose-phosphate-maltose (SAGP-maltose) quadruple-pack system was devised to improve the conventional method of preparing blood components. One of the features of this method is that high-speed centrifugation was used as the initial spin in the two-step centrifugation of whole blood. This feature permitted the removal of the buffy coat from red cells at the same time the platelets were isolated. Since the platelets were concentrated without forming a button, the damage to the platelets due to mechanical stress in preparation was minimized. Another major feature was the use of the SAGP-maltose solution. Maltose effectively prevented hemolysis during storage in a protein-poor medium for preserving red cells. Judging from the red cell adenosine triphosphate level and morphology score, it should be possible to store red cells for 35 to 42 days in SAGP-maltose preservative. In addition, approximately 25 percent more plasma was collected in the present system than in conventional multiple-pack systems.

Studies on the Procurement of Blood Coagulation Factor VIII in vitro Studies on Blood Components Prepared in Half‐Strength Citrate Anticoagulant

“…Platelets were counted electronically (Coulter Thrombocounter), hypotonic shock response determined as described by Tandy and Taylor [25]. Electron microscopy of platelets was performed according to Archer et al [26], and red cell morphology assessed by light microscopy as described by Uda et al [27], based on cell forms defined by Bessis [28]. Sterility testing was performed in a manner similar to that suggested by Puckett [29].…”

Studies on the Procurement of Blood Coagulation Factor VIII in vitro Studies on Blood Components Prepared in Half-Strength Citrate Anticoagulant

“…Citrate was assayed colorimetrically [24], Platelets were counted electronically (Coulter Thrombocounter), hypotonic shock response determined as described by Tandy and Taylor [25]. Electron microscopy of platelets was per formed according to Archer et al [26], and red cell morphology assessed by light microscopy as described by Uda et al [27], based on cell forms defined by Bessis [28]. Sterility testing was performed in a manner similar to that suggested by Puckett [29].…”

The preservative‐exchange method using a sextuple‐bag system for a 10‐week storage period of red blood cells

“…Preparation and preseroation of red blood cells with u sextuple-hag system Figure 1 shows the sextuple-bag system (Kawasumi Kagakukogyo Co., Ltd, Tokyo, Japan) according to our specifications: the primary bag contains 56 ml of citrate-phosphate-glucose (CPD) solution, and the third to fifth bags contain 140 ml of SAGP-maltose solution (Uda et al, 1985) composed of 2 mM adenine, 50 mM glucose, 20 mM disodium hydrogen phosphate, 60 mM maltose, and approximately 80 mM sodium chloride, with an osmotic pressure of approximately 300 mOsm. The SAGP-maltose solution was sterilized separately by filtration through a 0.22-pm membrane filter (Japan Milipore Co., Ltd, GSWP 04700, Tokyo, 257 Japan), and then added to the third to fifth satellite bags of the previously autoclaved sextuple-bag system.…”

“…ATP, ADP and AMP were measured spectrophotometrically according to an enzymatic method (Jaworek et a[., 1974). A morphology score was determined as described previously (Uda et al, 1985;Ohkuma & Uda, 1986). Briefly, a morphology score was calculated from the percentage of red cells of each of four morphological types, according to the modification of the classification of Bessis (Brecher & Bessis, 1972), and given an integral value from 3 to 0 (discocyte = 3, echinocyte I = 2, echinocyte I1 = 1, spherocyte = 0).…”

“…In order to solve this problem, we devised a method to preserve red cells in a liquid state by replacing the preservative using a sextuple-bag system with a saline-adenine-glucose-phosphatemaltose (SAGP-maltose) solution (Uda et al, 1985) as preservative. It is possible to exchange the preservative three times in this closed system.…”