Preparation of Bionanoreactor Based on Core−Shell Structured Polyion Complex Micelles Entrapping Trypsin in the Core Cross-Linked with Glutaraldehyde

Abstract

Recently, the polyion complex (PIC) micelle has been suggested as a promising carrier system for peptide and proteins. However, its utilities are limited by its sensitivity to the environment such as dilution and ionic strength of the solution. In this study, to overcome these obstructions, PIC micelles prepared from an anionic block copolymer, poly(ethylene glycol)-poly(alpha,beta-aspartic acid), and a cationic protein, trypsin, were cross-linked with glutaraldehyde through the Schiff base formation. On the basis of a light scattering technique, the results revealed an efficient resistance of the cross-linked PIC micelle to a high salt concentration, which was a key parameter controlling the structure of the PIC micelles. Moreover, the stability of trypsin after cross-linking was remarkably improved. Evidently, as a bionanoreactor and/or bionanoreservoir, the PIC micelles entrapping protein molecules in the cross-linked core reveal an improved stability, allowing their wide application in the fields of biotechnology and pharmaceutical sciences.

Preparation, characterization and application of star-shaped PCL/PEG micelles for the delivery of doxorubicin in the treatment of colon cancer

“…Nanoparticles with high CMC disaggregate during dilution, leading to a fast burstrelease of drugs. Thus, polymeric nanoparticles with low CMC attract some attention as drug delivery systems, and some attempts have been made to reduce the CMC of micelles, with use of nuclear cross-linked micelles, 15,16 shell cross-linked micelles, 17 and so on. The monomethoxy poly (ethylene glycol)-poly(ε-caprolactone) (MPEG-PCL) diblock copolymer is composed of a monomethoxy poly (ethylene glycol) (MPEG) segment and poly(ε-caprolactone) (PCL) segment.…”

A Macrophage−Nanozyme Delivery System for Parkinson's Disease

“…Therefore, it is crucial to protect the activity of the enzyme inside of the cell-carrier. Incorporation into polymeric nanocarries (nanospheres, liposomes, micelles, nanoparticles) can provide such protection (50)(51)(52)(53)(54)(55)(56)(57)(58)(59)(60) (61)(62)(63). The enzyme polyelectrolyte complexes can be prepared at the nanoscale by self-assembly of enzymes with oppositely charged block polyelectrolytes containing ionic and nonionic water soluble blocks (64,65).…”

Novel Nanomaterials for Clinical Neuroscience

“…They are formed as a result of the reaction of double hydrophilic block copolymers containing ionic and nonionic blocks with macromolecules of opposite charge including oligonucleotides, plasmid DNA and proteins (Kabanov et al 1995;Harada and Kataoka 1999a, b;Nguyen et al 2000;Zhang et al 2003;Jaturanpinyo et al 2004), or surfactants of opposite charge (Bronich et al 1997(Bronich et al , 1998(Bronich et al , 1999Solomatin et al 2003Solomatin et al , 2004. For example, block ionomer complexes were prepared by reacting trypsin or lysozyme (that are positively charged under physiological conditions) with an anionic block copolymer, PEG-poly(α,β-aspartic acid) (Harada and Kataoka 1999b;Jaturanpinyo et al 2004).…”