Preparation of biomimetic three-dimensional gelatin/montmorillonite–chitosan scaffold for tissue engineering

Zheng, Jun; Wang, Chuan Zeng; Wang, Xiu Xing; Wang, Hong Yan; Zhuang, Hong; Yao, Kang

doi:10.1016/j.reactfunctpolym.2006.12.002

Cited by 120 publications

(40 citation statements)

References 25 publications

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“…This structure is formed by an initial liquid-liquid phase separation forming the vertical channels while localised depletion of the polymer from the solution ahead of an additional phase separation front leads to the creation of the horizontal tubular pores [46]. Furthermore, the structure for the neat PVA/PAA foam featured much smaller pores compared to the foam with 10wt.% sepiolite clay, which agrees with the results for chitosan/titania and poly(Llactic) acid/bioglass nanocomposite foams [47,48] but in contradiction to the work for polyurethane/fluor-hydroxyapatite [49], gelatin/sepiolite [38] as well as gelatin/ montmorillonite/chitosan nanocomposite foams [50]. Pore size and pore structure are controlled by the ice crystals formed during the freeze-drying process, which are dependent on the freezing and drying conditions (e.g.…”

Section: Morphologiessupporting

confidence: 72%

Porous poly(vinyl alcohol)/sepiolite bone scaffolds: Preparation, structure and mechanical properties

Killeen

Frydrych

Chen

2012

Materials Science and Engineering: C

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Porous poly(vinyl alcohol) (PVA)/sepiolite nanocomposite scaffolds containing 0-10wt.% sepiolite were prepared by freeze-drying and thermally crosslinked with poly(arylic acid).The microstructure of the obtained scaffolds was characterised by scanning electron microscopy and micro computer tomography, which showed a ribbon and ladder like interconnected structure. The incorporation of sepiolite increased the mean pore size and porosity of the PVA scaffold as well as the degree of anisotropy due to its fibrous structure.The tensile strength, modulus and energy at break of the PVA solid material that constructed the scaffold were found to improve with additions of sepiolite by up to 104%, 331% and 22% for 6wt.% clay. Such enhancements were attributed to the strong interactions between the PVA and sepiolite, the good dispersion of sepiolite nanofibres in the matrix and the intrinsic properties of the nanofibres. However, the tensile properties of the PVA scaffold deteriorated in the presence of sepiolite because of the higher porosity, pore size and degree of anisotropy.The PVA/sepiolite nanocomposite scaffold containing 6wt.% sepiolite was characterized by an interconnected structure, a porosity of 89.5% and a mean pore size of 79μm and exhibited a tensile strength of 0.44MPa and modulus of 14.9MPa, which demonstrates potential for this type of materials to be further developed as bone scaffolds.

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Section: Morphologiessupporting

confidence: 72%

Porous poly(vinyl alcohol)/sepiolite bone scaffolds: Preparation, structure and mechanical properties

Killeen

Frydrych

Chen

2012

Materials Science and Engineering: C

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“…In comparison with previous studies, [31,49,55,63] it can be concluded that the CS-Gel/nHA-PANI scaffold-containing PANI as a conducting polymer showed absolutely non-toxic effects in vitro, and also its properties can be controlled by synergetic effect of nHA and PANI.…”

Section: Cell Viability Studiesmentioning

confidence: 43%

“…Also the chains of gelatin, a hydrophilic polymer (presence of amide and carboxyl groups), hydrolyse quickly in the presence of water. [55] In CS-Gel/nHA composite scaffold, the degradation was decreased by the addition of nHA compared to the CS-Gel matrix. This is due to the reduction of the accessibility of buffer media to the attacking sites in polysaccharide molecules.…”

Section: In Vitro Biodegradation Studiesmentioning

confidence: 96%

Fabrication and characterization of chitosan–gelatin/nanohydroxyapatite–polyaniline composite with potential application in tissue engineering scaffolds

Azhar

Olad

Salehi

2014

Designed Monomers and Polymers

114

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In this study to further improve surface characteristics of tissue engineering scaffolds, a novel composite based on chitosan-gelatin/nanohydroxyapatite-polyaniline (CS-Gel/nHA-PANI) was prepared by blending the synthesized nHA and PANI with chitosan and gelatin solution followed by lyophilization technique. The prepared composite scaffold was characterized using Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy techniques. The effects of nHA and PANI on the physicochemical properties of the scaffold including swelling ratio, density, biodegradation, biomineralization, and mechanical behavior were investigated. The composite scaffold was highly porous with the mean pore size of 200 μm. Compared with CS-Gel and CS-Gel/nHA scaffolds, the CS-Gel/nHA-PANI scaffold exhibited lower degradation rate and higher biomineralization and mechanical strength. Preliminary evaluation of the biocompatibility and cytotoxicity of the CS-Gel/nHA-PANI composite scaffold was performed by MTT assay using bovine leukemia virus fetal lamb kidney cell line (BLV-FLK) and then cell attachment studies were assessed using dental pulp stem cells. Results indicated no toxicity, and cells attached and proliferated on the pore surfaces of the scaffold. These findings suggested that the developed scaffold possess the prerequisites and can be used as a scaffold for hard tissues regeneration.

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“…225 A similar behaviour was found in silicate-based hybrids, for instance in the assembly of silk fibroin to wollastonite, a calcium silicate mineral, resulting in a biohybrid material with higher bioactivity than the pristine protein scaffold. 226 Following a blocks-assembly approach, layered silicates of the smectite family [227][228][229] or fibrous clays such as sepiolite 215,223,230 have been explored as the inorganic reinforcing component of biohybrids, in an attempt to develop new materials mimicking natural bone or similar tissues. Recent examples reveal different roles of the silicate platelets or fibres in the resulting biohybrids.…”

Section: Structural Proteins-based Silica and Silicate Biohybridsmentioning

confidence: 99%

“…10 MPa. 226,227,229 Gelatin-silica biohybrids were also processed as porous scaffolds, showing a bimodal distribution of pores (Fig. 11), and successfully tested for in vitro cell culture, 232,233 but none of these studies report on the mechanical properties of these materials.…”

Section: Structural Proteins-based Silica and Silicate Biohybridsmentioning

confidence: 99%

Hybrid and biohybrid silicate based materials: molecular vs. block-assembling bottom–up processes

2011

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Preparation of biomimetic three-dimensional gelatin/montmorillonite–chitosan scaffold for tissue engineering

Cited by 120 publications

References 25 publications

Porous poly(vinyl alcohol)/sepiolite bone scaffolds: Preparation, structure and mechanical properties

Porous poly(vinyl alcohol)/sepiolite bone scaffolds: Preparation, structure and mechanical properties

Fabrication and characterization of chitosan–gelatin/nanohydroxyapatite–polyaniline composite with potential application in tissue engineering scaffolds

Hybrid and biohybrid silicate based materials: molecular vs. block-assembling bottom–up processes

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