Preparation of Biodegradable Polymer Nanoparticles by Miniemulsion Technique and Their Cell Interactions

Abstract

The emulsion/solvent evaporation method and miniemulsion technique were combined and applied in the formulation of biodegradable monodisperse nanoparticles at high solid contents using different biocompatible and biodegradable polymers such as poly(L-lactide) (PLLA), poly[(D,L-lactide)-co-glycolide] 50:50 (PLGA), and poly(epsilon-caprolactone) (PCL). Differences between the results of various polymers are found in terms of the particle size and size distribution as well as in the degradation time. An encapsulated hydrophobic fluorescent dye was used as a model marker in order to study the entrapment efficiency and diffusion yield out of the particle. Cellular uptake of the obtained particles was observed in Jurkat and HeLa cells. In the investigated particle size range of 80-200 nm, the surfactant on the particles' surface had a greater influence than the particle size. Uptake kinetics reveals that the PLLA and PCL particles are endocytosed much faster than polystyrene particles.

Characterization of progesterone loaded biodegradable blend polymeric nanoparticles

“…The average nanoparticles size obtained by the authors remained between 247 and 1070nm using poly (vinil alcohol) as surfactant. MUSYANOVYCH et al (2008) produced PCL nanoparticles with lutensol as surfactant and obtained similar results as those presented in table 1. Figure 1 presents the infrared spectra of all the components used in the nanoparticles preparation along with some characteristic absorption bands.…”

“…Nanoparticles of pure PHBV, PCL and PLLLA and blend nanoparticles of PHBV/PCL and PHBV/PLLA were prepared as described by MUSYANOVYCH et al (2008). The organic phase was prepared dissolving lecithin (0.175g), polymer (0.3g) and progesterone (5mg or 0mg) in chloroform (10g).…”

From polymeric particles to multifunctional nanocapsules for biomedical applications using the miniemulsion process

“…36,37 The combination of emulsion/solvent evaporation method and miniemulsion technique was applied to obtain biodegradable polyester-based nanoparticles from preformed polymer. 38 This technique is shown in Figure 2.…”

“…By the variation of functional groups and their density on the particle surface, it was clearly demonstrated that the surface groups significantly impart the rate of cellular uptake. 18,38,100 The functionalization of well-defined PU nanocapsules with an aqueous core prepared by performing a polyaddition at the interface of inverse miniemulsion droplets could be demonstrated. 101 The miniemulsion technique involving the nanoreactor concept allows one to obtain an encapsulation efficiency as high as 90% within the nanocapsules.…”

“…Besides the above-mentioned experiments using PS-based nanoparticles, N-(2,6-diisopropylphenyl)perylene-3,4-dicarbonacidimide could be successfully incorporated in phosphatefunctionalized poly(methyl methacrylate) (PMMA) and PS, 78 polyisoprene (PI), PS-co-PI, 117 polyester, 38 and PBCA 118 particles for the investigation of cellular response to these polymeric nanoparticles. It could be shown that the internalization in different cell lines depends on the cell line, the polymer and their surface functionalization.…”