Preparation of Biocompatible, UV-Cured Fumarated Poly(ether-ester)-Based Tissue-Engineering Hydrogels

Abstract

The aim of this study was to develop biodegradable, photo-polymerizable in situ gel-forming systems prepared from a fumaric acid monoethyl ester (FAME) modified poly(lactide-co-glycolide) (PLGA) co-polymer. By reacting lactide and glycolide in the presence of stannous octoate as a catalyst and 2-ethyl,2-hydroxymethyl 1,3-propanediol as an initiator, hydroxyl terminated branched PLGA was synthesized. Afterwards, at room temperature hydroxyl terminated branched PLGA was reacted with fumaric acid monoethyl ester (FAME). N,N'-dicyclohexylcarbodiimide and triethylamine were used as a coupling agent and catalyst, respectively. The gel percentage, equilibrium mass swelling, degradation profile and polymerization kinetics of the hydrogels were investigated. All of the results were influenced by the amount of FAME modified PLGA co-polymer. Biocompatibility of the hydrogels was examined by using MTT cytotoxicity assay. According to the results, hydrogels are biocompatible and cell viability percentage depends on the amount of PLGA co-polymer. While the amount was 15% in hydrogel composition, cell viability was 100%, but after increasing the PLGA co-polymer amount to 30% the viability reduced to 78%.

Formulation and Characterization of Gelatin-Based Hydrogels for the Encapsulation of Kluyveromyces lactis—Applications in Packed-Bed Reactors and Probiotics Delivery in Humans

“…This behavior has been observed in ceramic materials such as bricks and glasses [ 89 ]. This behavior is somewhat counterintuitive as we expected that highly crosslinked hydrogels exhibited the highest resistance to rupture [ 13 , 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 ,…”

“…Hydrogels are, however, subject of instability and macroscopic deformation, particularly when subjected to a considerable degree of swelling [ 13 ]. These attributes have been crucial for the development of several applications, including industrial biotransformation, antimicrobial peptide production, cell encapsulation, water and air purification and medical applications such as tissue engineering and cell therapy [ 14 , 16 , 17 , 18 , 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 ].…”

Fabrication of collagen immobilized electrospun poly (vinyl alcohol) scaffolds

“…The reaction mixture was stirred gently at 40°C for 24 hr. The hydroxyl groups of PVA reacted with CDI . N‐Carbonyl imidazol‐tethered nanofiber was gently shaken in collagen solution with a concentration of 2 mg/ml acetic acid at room temperature for 24 hr.…”

Hydrogels: Smart Materials for Drug Delivery

“…They provide fibrous three dimensional bases and other applications like bone defect restoration in tissue engineering. 21 Akdemir et al 22 also prepared biocompatible and UV cured fumarated poly (ether-ester)-based tissue engineering hydrogels. The modification of the poly (lactide-co-glycolide) (PLGA) copolymer was done using stannous octoate as a catalyst.…”