Preparation of Biocompatible Carboxymethyl Chitosan Nanoparticles for Delivery of Antibiotic Drug

Abstract

Objective. To prepare biocompatible ciprofloxacin-loaded carboxymethyl chitosan nanoparticles (CCC NPs) and evaluate their cell specificity as well as antibacterial activity against Escherichia coli in vitro. Methods. CCC NPs were prepared by ionic cross-linking method and optimized by using Box-Behnken response surface method (BBRSM). Zeta potential, drug encapsulation, and release of the obtained nanoparticles in vitro were thoroughly investigated. Minimum inhibitory concentration (MIC) and killing profiles of free or ciprofloxacin-loaded nanoparticles against Escherichia coli were documented. The cytotoicity of blank nanoparticles and cellular uptake of CCC NPs were also investigated. Results. The obtained particles were monodisperse nanospheres with an average hydrated diameter of 151 ± 5.67 nm and surface of charge −22.9 ± 2.21 mV. The MICs of free ciprofloxacin and CCC NPs were 0.16 and 0.08 μg/mL, respectively. Blank nanoparticles showed no obvious cell inhibition within 24 h, and noticeable phagocytosis effect was observed in the presence of CCC NPs. Conclusion. This study shows that CCC NPs have stronger antibacterial activity against Escherichia coli than the free ciprofloxacin because they can easily be uptaken by cells. The obtained CCC NPs have promising prospect in drug delivery field.

Solid lipid nanoparticles for thermoresponsive targeting: evidence from spectrophotometry, electrochemical, and cytotoxicity studies

“…Nanoparticles with a zeta potential between 20 and 40 mV will have sufficient repulsion to remain homogeneously dispersed in the aqueous dispersion. 29 The size of 5-FUloaded lauric acid and oleic acid nanoparticles prepared with mono-surfactant system (FLOM), 5-FU-loaded lauric acid and oleic acid nanoparticles prepared with double-surfactant system (FLOD) and 5-FU loaded lauric acid and linoleic acid nanoparticles prepared with double-surfactant system (FLLD) was ,200 nm (polydispersity index [PDI] ,0.2), and zeta potential was ,−30 mV in all cases, which is desirable in drug delivery applications (Table 1). We confirmed thermoresponsive nature of as-prepared SLNs by light transmission studies.…”

Enhanced Neuroprotection of Acetyl-11-Keto-β-Boswellic Acid (AKBA)-Loaded O-Carboxymethyl Chitosan Nanoparticles Through Antioxidant and Anti-Inflammatory Pathways

“…They can open tight junctions between intestinal epithelial cell and facilitate paracellular transport of drugs transiently [19]. Due to poor solubility of chitosan in an aqueous solution with neutral pH, we applied O-carboxymethyl chitosan (O-CMC), a watersoluble chitosan derivative, which has favorable biocompatibility like chitosan [20,21].…”

Efficient induction of antimicrobial activity with vancomycin nanoparticle-loaded poly(trimethylene carbonate) localized drug delivery system

“…14 There were negative charges on the surface of antibiotic-loaded CMC NPs, and burst release of the drug in the initial stage was observed. 15 In our study, CS was derivatized by N-methylation to N,N,N-trimethyl chitosan (TMC), a water-soluble compound, to form nanocomplexes with anionic compounds, such as drugs, proteins and DNA, through ionotropic gelation as opposed to a pH-dependent charge. 16 VCM-loaded TMC NPs (VCM/TMC NPs), prepared in our previous study, 17 proved to be an effective intracellular mode of drug administration.…”