Preparation of Bi_0.15Fe_0.15TiO₂ Nanocomposites for the Highly Selective Enrichment of Phosphopeptides

Abstract

Novel Bi 0.15 Fe 0.15 TiO 2 nanocomposites (B 0.15 F 0.15 TNs) were synthesized for the first time by a modified sol−gel technology and successfully applied to selective extraction and enrichment of phosphopeptides from digested protein mixture solutions and real samples (tissue protein extract from human liver). The codoping of Bi and Fe into TiO 2 results in a significant enhancement in both the enrichment efficiency and selectivity. Compared with the commercial available TiO 2 extractant, the proposed B 0.15 F 0.15 TNs possess a lower detection limit (2 × 10 −9 M) and higher selectivity at a low weight ratio of 1:1200 (phosphopeptides/nonphosphopeptides). Additionally, a total of 223 phosphorylation sites were identified from the human liver lysate after enrichment by the B 0.15 F 0.15 TNs. In addition, the synthesis of B 0.15 F 0.15 TNs is quite easy, of high yield, and inexpensive.

Phosphopeptide enrichment for phosphoproteomic analysis - A tutorial and review of novel materials

“…Remarkably, composites containing different metal oxide/ions precursors demonstrated effective p-peptide enrichment capacity (supporting Table S3, for instance, 746 TiO2/Bi/Fe/Zr [58], B0.15F0.15TNs [59], and MnFe2O4 MAMSs [75]).…”

“…Key factors affecting MOAC binding performance (Fig. 1b) towards p-peptides include properties of the material: surface area, pore size, 633 isoelectric point (IEP) and magnetic properties, and the presence of additives [43,58,59]. Among these, IEP is considered to be the most critical parameter for the enrichment performance of affinity materials.…”

“…Compared to the commercial available TiO 2, the co-doping of metal (ions Bi 3+ , Fe 3+ and Zr 4+ ) with TiO 2 nanocomposite results in an increased surface area, which leads to a lower detection threshold for casein/BSA, and a higher detection number of p-peptides (26% more for HeLa cells using TiO 2 /Bi/Fe/Zr) [58] and phospho-sites (two-fold more for 751 tissue protein extract from human liver using B 0.15 F 0.15 TNs) [59]. Similarly, the combined usage of ferric and manganous ions as precursors, in the novel synthesized MnFe2O4 MAMSs microspheres, showed a higher selectivity for p-peptides than Fe3O4 nanoparticles or MnOOH nanosheets individually [75] [77] proved to be superior to the individual metal oxides, demonstrated by their stronger specificity and higher selectivity for p-peptide enrichment.…”

Nanomaterials in Proteomics

“…The structural diagram of CaCuSi 4 O 10 was displayed in Figure E. Furthermore, Bi 0.15 Fe 0.15 TiO 2 nanomaterials were prepared via the sol‐gel approach, the structural diagram of Bi 0.15 Fe 0.15 TiO 2 was displayed in Figure F . Bi 0.15 Fe 0.15 TiO 2 nanomaterials exhibited significant enhancement of capture performance compared to commercial TiO 2 , which was attributed to the codoping of Bi and Fe.…”

Graphene oxide–metal oxide nanocomposites for on‐target enrichment and analysis of phosphorylated biomolecules

“…Phosphopeptides/phosphoproteins are enriched through specific affinity and analyzed by MS. Metal oxide affinity chromatographic media reversibly interacts with phosphates through hydroxyl groups of metal oxides [3]. Metal oxides of transition metals (TiO 2 [4], ZrO 2 [5], and Ta 2 O 5 [6]) and lanthanides (La 2 O 3 [7], HfO 2 [8], and CeO 2 [9]) as micro‐ and nano‐materials or composites enrich phospho‐moieties via solid‐phase batch extraction [10]. Composites of graphene oxide (GO) with metal oxides such as TiO 2 [11], nickel oxide [12], and molybdenum oxide [13] enrich phosphopeptides through batch extraction.…”