Preparation of Armored RNA as a Control for Multiplex Real-Time Reverse Transcription-PCR Detection of Influenza Virus and Severe Acute Respiratory Syndrome Coronavirus

Yu, Xin; Pan, Jing; Rong, Yue; Xiang, Hai Qing; Yu, Keming; Huang, Zhi Cheng

doi:10.1128/jcm.01904-07

Cited by 36 publications

(40 citation statements)

References 18 publications

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“…Many researchers have produced armored RNA that is nuclease-resistant by the MS2 bacteriophage (Huang et al 2006;Konnick et al 2005;Pasloske et al 1998;Sun et al 2013;Wei et al 2008a, b;Yu et al 2008;Zhan et al 2009). Our team has made continuous progress in this aspect.…”

Section: Discussionmentioning

confidence: 99%

A novel method to produce armored double-stranded DNA by encapsulation of MS2 viral capsids

Zhang

Sun

Chang

et al. 2015

Appl Microbiol Biotechnol

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With the rapid development of molecular diagnostic techniques, there is a growing need for quality controls and standards with favorable properties to monitor the entire detection process. In this study, we describe a novel method to produce armored hepatitis B virus (HBV) and human papillomavirus (HPV) DNA for use in nucleic acid tests, which was confirmed to be stable, homogeneous, noninfectious, nuclease resistant, and safe for shipping. We demonstrated that MS2 bacteriophage could successfully package double-stranded DNA of 1.3-, 3-, 3.5-, and 6.5-kb length into viral capsids with high reassembly efficiency. This is the first application of RNA bacteriophage MS2 as a platform to encapsulate double-stranded DNA, forming virus-like particles (VLPs) which were indistinguishable from native MS2 capsids in size and morphology. Moreover, by analyzing the interaction mechanism of pac site and the MS2 coat protein (CP), we found that in addition to the recognized initiation signal TR-RNA, TR-DNA can also trigger spontaneous reassembly of CP dimers, providing a more convenient and feasible method of assembly. In conclusion, this straightforward and reliable manufacturing approach makes armored DNA an ideal control and standard for use in clinical laboratory tests and diagnostics, possessing prospects for broad application, especially providing a new platform for the production of quality controls for DNA viruses.

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Section: Discussionmentioning

confidence: 99%

A novel method to produce armored double-stranded DNA by encapsulation of MS2 viral capsids

Zhang

Sun

Chang

et al. 2015

Appl Microbiol Biotechnol

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“…However, such problems could be well avoided in real-time RT-PCR assays where EPC is an important component. Yu et al [17] developed a real-time RT-PCR assay in which the armored RNA was used as EPC to detect the SARS-CoV, with a detection limit of 10 copies/mL. Meanwhile, the rapidly mutating nature of coronaviruses highlights the need for accurate detection of genetically diverse coronaviruses.…”

Section: Pcr-based Methodsmentioning

confidence: 99%

Recent advances and perspectives of nucleic acid detection for coronavirus

Shen

Zhou

et al. 2020

Journal of Pharmaceutical Analysis

473

423

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Keywords:Coronavirus Nucleic acid detection PCR-Based methods Isothermal nucleic acid amplification-based methods Microarray-based methods a b s t r a c tThe recent pneumonia outbreak caused by a novel coronavirus (SARS-CoV-2) in Chinais posing a great threat to global public health. Therefore, rapid and a accurate identification of pathogenic viruses plays a vital role in selecting appropriate treatments, saving people's lives and preventing epidemics. It is important to establish a quick standard diagnostic test for the detection of the infectious disease (COVID-19) to prevent subsequent secondary spread. Polymerase chain reaction (PCR) is regarded as a gold standard test for the molecular diagnosis of viral and bacterial infections with high sensitivity and specificity. Isothermal nucleic acid amplification is considered to be a highly promising candidate method due to its fundamental advantage in quick procedure time at constant temperature without thermocycler operation. A variety of improved or new approaches also have been developed. This review summarizes the currently available detection methods for coronavirus nucleic acid. It is anticipated that this will assist researchers and clinicians in developing better techniques for timely and effective detection of coronavirus infection.

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“…Since these initial results, Armored RNA has been applied as a control for a vast array of assays, which include the detection of HCV, 404-406 respiratory viruses such as influenza and severe acute respiratory syndrome (SARS) viruses, 407-409 enteroviruses, 410, 411 and West Nile virus. 412 It has even been utilized as a surrogate virus for the detection of animal pathogens, including classical swine fever virus (CSFV), FMDV, and vesicular stomatitis virus (VSV).…”

Section: Applications Of Virus-based Particlesmentioning

confidence: 99%

Design of virus-based nanomaterials for medicine, biotechnology, and energy

2016

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Virus-based nanomaterials are versatile materials that naturally self-assemble and have relevance for a broad range of applications including medicine, biotechnology, and energy. This review provides an overview of recent developments in “chemical virology.” Viruses, as materials, provide unique nanoscale scaffolds that have relevance in chemical biology and nanotechnology, with diverse areas of applications. Some fundamental advantages of viruses, compared to synthetically programmed materials, include the highly precise spatial arrangement of their subunits into a diverse array of shapes and sizes and many available avenues for easy and reproducible modification. Here, we will first survey the broad distribution of viruses and various methods for producing virus-based nanoparticles, as well as engineering principles used to impart new functionalities. We will then examine the broad range of applications and implications of virus-based materials, focusing on the medical, biotechnology, and energy sectors. We anticipate that this field will continue to evolve and grow, with exciting new possibilities stemming from advancements in the rational design of virus-based nanomaterials.

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Preparation of Armored RNA as a Control for Multiplex Real-Time Reverse Transcription-PCR Detection of Influenza Virus and Severe Acute Respiratory Syndrome Coronavirus

Cited by 36 publications

References 18 publications

A novel method to produce armored double-stranded DNA by encapsulation of MS2 viral capsids

A novel method to produce armored double-stranded DNA by encapsulation of MS2 viral capsids

Recent advances and perspectives of nucleic acid detection for coronavirus

Design of virus-based nanomaterials for medicine, biotechnology, and energy

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