Preparation of aqueous sol–gel silica and titania multi-layered thin films and their evaluation as biomolecular encapsulation hosts

optical catechol biosensor based on a desert truffle tyrosinase extract immobilized into a sol-gel silica layered matrix. AbstractAn optical biosensor for the determination of catechol, a widely used yet toxic and carcinogenic molecule, is proposed using a crude extract of desert truffle (Terfezia leonis Tul.)as an enzymatic source of tyrosinase. The biosensor is constructed by the immobilization of tyrosinase crude extract in a bi-layered silica gel film prepared by dip-coating of an alkoxide/colloidal silica solution containing the enzyme on glass slide. Encapsulation has a moderate effect of the enzyme optimal pH stability but largely increases its thermal stability.Immobilized enzymes have a higher substrate affinity towards catechol but smaller maximum conversion velocity. The optical biosensor provides a linear response for catechol in the concentration range of 50-400 µM and a limit of detection was 52 µM. AFM studies show that the enzymes impact on the silica gel structure, preventing further deposition of additional layers. Comparison with similar dopamine biosensors points out that the impact of encapsulation on enzymatic activity may depend on the considered substrate.

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An optical catechol biosensor based on a desert truffle tyrosinase extract immobilized into a sol–gel silica layered matrix

Leboukh

Gouzi

Coradin

et al. 2018

J Sol-Gel Sci Technol

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Immobilization of a Polyphenol Oxidase Extract from Terfezia leonis Tul. Desert Truffle in Multilayered Silica Films for Dopamine Biosensing

Gouzi

Moreau

Depagne

et al. 2013

Silicon

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Sol-gel Encapsulation of Biomolecules and Cells for Medicinal Applications

Wang¹,

Ahmed²,

Álvarez³

et al. 2015

CTMC

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The sol-gel process provides a robust and versatile technology for the immobilization of biologicals. A wide range of inorganic, composites and hybrid materials can be prepared to encapsulate molecular drugs, proteins, antibodies/antigens, enzymes, nucleic acids, prokaryotic and eukaryotic cells into bulk gels, particles and films. This review describes the applications of sol-gel encapsulation relevant to medicinal chemistry focusing on the recent development of biosensors as well as systems for production, screening and delivery of bioactive compounds and biomaterials.

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Preparation of aqueous sol–gel silica and titania multi-layered thin films and their evaluation as biomolecular encapsulation hosts

Cited by 3 publications

References 47 publications

An optical catechol biosensor based on a desert truffle tyrosinase extract immobilized into a sol–gel silica layered matrix

An optical catechol biosensor based on a desert truffle tyrosinase extract immobilized into a sol–gel silica layered matrix

Immobilization of a Polyphenol Oxidase Extract from Terfezia leonis Tul. Desert Truffle in Multilayered Silica Films for Dopamine Biosensing

Sol-gel Encapsulation of Biomolecules and Cells for Medicinal Applications

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